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Microfluidic optical observation system and observation method for small molecule chemical trend behavior

An optical observation and microfluidic technology, applied in the field of simulated chemical analysis, can solve the problems of difficult to fully reflect the molecular kinematics and dynamic effects, and achieve highly feasible results.

Pending Publication Date: 2021-10-12
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The discovery of these peculiar phenomena shows that it is difficult for traditional theories to fully reflect the effects of molecular kinematics and dynamics during the energy exchange in the reaction, which leads to the fact that the real distribution of various types of ensembles in space-time and their fluctuations in time and space will change over a period of time during the reaction. The inside is covered up, which may hide the chemical tendency of the molecule
However, there is no literature report on the observation of small molecule chemotaxis behavior.

Method used

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  • Microfluidic optical observation system and observation method for small molecule chemical trend behavior
  • Microfluidic optical observation system and observation method for small molecule chemical trend behavior
  • Microfluidic optical observation system and observation method for small molecule chemical trend behavior

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Embodiment 1

[0043] This embodiment provides a microfluidic chip system for observing the cohesive chemotaxis behavior of ZnTSPP affected by imidazole ligands. The specific steps are as follows:

[0044] (1) Prepare 10 mM PBS buffer solution with pH 9.0, and use this solution to prepare receptor solution A: 10 μM ZnTSPP solution, and ligand solution B: 100 μM imidazole solution;

[0045] (2) Fully rinse the microfluidic channel with a buffer solution;

[0046] (3) According to the configuration order of the three inlets from left to right, the microchannel is respectively passed into PBS / ZnTSPP / PBS, and the fluorescence data of the inlet confluence point of the microfluidic channel and the sampling point at 4.7 mm downward from the inlet are recorded (such as image 3 Line (a) in B and 3C);

[0047] (4) According to the configuration order of the three inlets from left to right, the microchannel is respectively fed into PBS / ZnTSPP+imidazole / PBS, and the fluorescence data of the inlet conf...

Embodiment 2

[0050] This embodiment provides a flow rate optimization experiment of a microfluidic chip system for observing the chemotaxis behavior of small molecule receptors. The specific steps are as follows:

[0051] (1) Prepare 10 mM PBS solution with pH 9.0, and use the solution to prepare receptor solution A: 10 μM ZnTSPP solution, and ligand solution B: 100 μM imidazole solution;

[0052] (2) Fully rinse the microfluidic channel with a buffer solution;

[0053] (3) The microchannels are respectively connected to PBS / ZnTSPP / PBS according to the configuration order of the three entrances from left to right;

[0054] (4) Control the flow rate to 1, 5, 10, 50, and 100 μL / h, respectively, and record the fluorescence intensity distribution of ZnTSPP at the confluence point in the lateral direction of the channel (such as Figure 5 A), and its relationship with flow rate (such as Figure 5 B). from Figure 5 It can be seen that the data point at the flow rate of 50 μL / h is the optima...

Embodiment 3

[0056] This embodiment provides a microfluidic optical observation system for the chemotaxis behavior of ZnTSPP influenced by imidazole ligands for directional migration. The specific steps are as follows:

[0057] (1) Prepare 10 mM PBS solution with pH 9.0, and use the solution to prepare receptor solution A: 10 μM ZnTSPP solution, and ligand solution B: 100 μM imidazole solution;

[0058] (2) Fully rinse the microfluidic channel with a buffer solution;

[0059] (3) According to the configuration order of the three inlets from left to right, the microchannels are respectively passed into PBS / ZnTSPP / PBS, and the fluorescence data of the sampling points at the 4.7mm downwards of the inlets of the microfluidic channels are recorded (such as Image 6 (a) line) among A and 6B), as blank control;

[0060] (4) According to the configuration order of the three inlets from left to right, the microchannel is respectively passed into imidazole / ZnTSPP / PBS, and the fluorescence data of t...

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Abstract

The invention discloses a microfluidic optical observation system and an observation method for a small molecule chemical trend behavior. The micro-fluidic optical observation system comprises an optical platform and a micro-fluidic chip, a photochemiluminescence substance is taken as a research object, a simulated chemical gradient field of an acceptor-ligand pair is constructed in a fluid with a low Reynolds number, and image acquisition is performed by utilizing the luminescence characteristic of the photochemiluminescence substance; and the spontaneous directional migration performance of molecules of different scales and ordered sets of the molecules is observed, motion phase state parameters of receptors are associated with reversible association reaction information of ligands, and the molecular binding induced chemotaxis phenomenon is observed in combination with mathematical statistics.

Description

technical field [0001] The invention belongs to the technical field of simulation chemical analysis, and relates to a microfluidic optical observation system and an observation method for the chemical tendency behavior of small molecules. Background technique [0002] For a long time, the empirical "collision theory" of classical thermodynamics has been used in textbooks to phenomenologically explain the molecular reaction process and its approximate dependence on macroscopic thermodynamic conditions, while the physical quantities such as reaction rate constants introduced in kinetics are only suitable for general description apparent process. But the fact is that the general reaction process, even if it has reached an equilibrium state or a non-equilibrium steady state, is bound to be accompanied by the evolution of a series of related states such as the speed, position, and orientation of a large number of molecules (ions), such as the 1977 Nobel Prize in Chemistry The th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64B01L3/00
CPCG01N21/6402G01N21/6458B01L3/5027B01L2200/10B01L2300/12
Inventor 邓盛元张煦彤马科锋李斌穆瑶康荣达刘四平
Owner NANJING UNIV OF SCI & TECH