Rose fungus and application thereof
A technology of rose fungus and bacterial agent, applied in the field of microorganisms, can solve the problems of ecological environment threat, non-biodegradable and other problems, and achieve the effect of good industrial application prospect
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Embodiment 1
[0035] Example 1 Separation and Screening of Rose Sn13-21
[0036] This embodiment provides the preparation process of rose bacteria SN13-21, reference figure 1 ,Specific steps are as follows:
[0037] 1, can cultivate the separation of strains
[0038] Collect the bottom mud of 10cm under the short sea seafront of Dapeng Bay Sea, Guangdong, China; add the collected sesamer samples to cone bottles with 100ml sterilized oil-containing salt enrichment medium, 30 ° C, 160R / min under temperature Swaying for 77 days, transferred to 1% inoculation of 1% every 7 days to a fresh medium-rich concentration medium, while increasing the oil content of 1%, salinity 10 ‰, cultivating the 7th, 42 and 77 days to take 1ml Soil suspension; dilute the bottom mud suspension gradient to make 10 -3 To 10 -5 Concentration soil suspension; adding the diluted undercut suspension to a 2216E medium plate for coating treatment, 30 ° C constant temperature incubator culture 48h to obtain colonies; picked a ...
Embodiment 2
[0043] Example 2 Identification of Rose Sn13-21
[0044] In this example, the rose Sn13-21 obtained in Example 1 was identified, including morphological observation, physiological biochemical identification, and 16S RDNA sequence analysis, as follows:
[0045] 1, morphological identification
[0046] The rose Sn13-21 scribblered in 2216E solid medium provided in Example 1 was inverted, and then the plate was inverted, and 24 h was cultured under temperature 30 ° C, and the growth of the colonies on the plate was observed. Reference on the colonies of rose bacteria SN13-21 image 3 . From image 3 It can be seen that the strain is pale yellow, circular, the edge is not neat, the surface is mucus, and the moist is smooth.
[0047] Rose Sn13-21 was subjected to Gram staining with the kit, and the strain was observed under an oil mirror, and the Grandstone dyeing picture of the strain Figure 4 . From Figure 4 It can be seen that the strain is red, which is Gram-negative bacteria.
[004...
Embodiment 3
[0061] Example 3 Determination of PHA capability of Rose Sn13-21
[0062] This embodiment has the measurement of the PHA capability of rose bacteria SN13-21, which includes the following:
[0063] 1.PHA extraction
[0064] The rose Sn13-21 obtained in Example 1 of the present invention was inoculated into a liquid medium, and the constant temperature shock was cultured for 4 days under 30 ° C, 150 r / min. After the end of culture, the yeast was taken at 5000 r / min for 20 min, and the cell precipitate was obtained, and then a freeze-drying treatment; 10 mg of bacteria lyophilized samples were weighed, then add 1 ml of chloroform (including 0.5 mg). / ML benzoate) and 1 ml contain a methanol solution containing 15% (v / v) concentrated sulfuric acid, sealing treatment under 100 ° C oil bath, methyl esterification reaction; after the reaction, the sample ice bath is cooled for 5 min Then, 0.5 ml of deionized water was added, mixed with 30s, 10 min at 3500r / min for 10 min, and th...
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