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A method for determining likelihood of an inflammatory gastrointestinal tract disease

A technology for gastrointestinal diseases and inflammatory bowel diseases, applied in the direction of disease diagnosis, preparation of test samples, biochemical equipment and methods, etc., can solve problems such as false positive results

Pending Publication Date: 2021-10-22
阿克森斯健康公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Also, use of anti-inflammatory medications can lead to false positive results and should therefore be discontinued within a few weeks of testing

Method used

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  • A method for determining likelihood of an inflammatory gastrointestinal tract disease
  • A method for determining likelihood of an inflammatory gastrointestinal tract disease

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] Accordingly, the present invention relates to a method for determining the likelihood of an inflammatory gastrointestinal disease comprising

[0031] - diluting a biological sample of a human subject in an aqueous solution to obtain a diluted sample;

[0032] - contacting said diluted sample with 8-anilinonaphthalene-1-sulfonic acid as a modifier to obtain a pre-measurement sample;

[0033] - forming a measurement sample by contacting said pre-measurement sample with a reagent comprising peroxidase and a label selected from europium chloride and terbium chloride;

[0034] - incubating said measurement sample;

[0035] - excite said measurement sample; and

[0036] - measuring a time-resolved luminescence signal of said tag in said measurement sample, and determining an increased likelihood of inflammatory bowel disease in said human subject if said luminescence signal is at least 112% higher than that of a control sample , the control sample is from a human subject n...

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PUM

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Abstract

The present invention relates to method for determining a likelihood of an inflammatory gastrointestinal tract disease. The method comprises diluting a biological sample of a human subject and contacting it with 8-anilinonaphthalene-1-sulfonic acid as modulating agent and further with a reagent, said reagent comprising a peroxidase enzyme and a label selected from a europium chloride and terbium chloride. The sample is incubated and excited, and the time-resolved luminescence signal of the label in the sample is measured. If the luminescence signal is at least 112% higher than for a control sample from a human subject without of an inflammatory intestine disease, an increased likelihood of an inflammatory intestine disease of the human subject is determined.

Description

technical field [0001] The present invention relates to methods for determining the likelihood of an inflammatory gastrointestinal disease from a biological sample of a human subject. Background technique [0002] Various inflammatory gastrointestinal tract diseases are known, such as irritable bowel syndrome (IBS) including Crohn's disease and ulcerative colitis. These disorders are currently thought to be related to oxidative stress in the gastrointestinal tract. [0003] IBS is a common condition that affects the digestive system and can cause symptoms such as stomach cramps, bloating, diarrhea and constipation. It is estimated that 45% of people worldwide are affected by IBS. Diagnosis is currently made by measuring calprotectin levels in stool. However, this measurement method is not fast or very reliable, i.e. it produces false positives and false negatives. For example, in the case of frequent rectal emptying, the amount of calprotectin may be normal, while the hu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N21/78G01N1/38
CPCG01N21/763G01N21/78G01N1/38G01N33/6893G01N33/533G01N2800/06G01N2800/52C12Q1/28G01N33/582G01N2800/065G01N2800/50G01N2800/7009
Inventor 詹妮·库尔帕科安妮塔·詹森里卡·埃尔基勒
Owner 阿克森斯健康公司