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Application of Verticillium dahliae vdprmt1 gene in improving disease resistance of crops or vegetables

A technology of Verticillium dahliae and genetics, applied in the direction of genetic engineering, plant genetic improvement, application, etc., can solve problems such as difficult prevention and control

Active Publication Date: 2021-12-21
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since Verticillium dahliae is a soil-borne phytopathogenic fungus, it is very difficult to control it, and there is no effective medicament in production at present

Method used

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  • Application of Verticillium dahliae vdprmt1 gene in improving disease resistance of crops or vegetables
  • Application of Verticillium dahliae vdprmt1 gene in improving disease resistance of crops or vegetables
  • Application of Verticillium dahliae vdprmt1 gene in improving disease resistance of crops or vegetables

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Experimental program
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Embodiment 1

[0046] Embodiment 1 Verticillium dahliae VdPRMT1 Screening of target gene fragments for genetic resistance to pathogenic bacteria, construction of interference vectors and application of transgenic tobacco for disease resistance

[0047] ⒈ Test material

[0048] ⑴ Materials

[0049] Tobacco: benthamiana ( Nicotiana benthamiana )strain.

[0050] Culture conditions: planted in mixed nutrient soil sterilized by high temperature and high pressure (Fangjie nutrient soil: vermiculite = 1:1), temperature 23 ± 2°C, relative humidity 75 ± 5%, photoperiod L:D is 16 h: 8 h.

[0051] ⑵ strains and plasmids

[0052] Cotton Verticillium dahliae pathogen: Verticillium dahliae ( Verticillium dahliae ) V991, a highly pathogenic deciduous strain, was donated by Jian Guiliang, a researcher at the Institute of Plant Protection, Chinese Academy of Agricultural Sciences.

[0053] Viral vector: tobacco rattle virus ( Tobacco rattle virus , TRV) binary vector (TRV1 and TRV2) was kindly donat...

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Abstract

The invention discloses Verticillium dahliae VdPRMT1 Use of genes in improving crop disease resistance. The present invention adopts the host-induced gene silencing technique and uses the strain of Verticillium dahliae as material to construct multiple VdPRMT1 The tobacco rattle virus interference plasmid of the gene was transformed into Nicotiana benthamiana and inoculated with Verticillium dahliae, and it was preliminarily determined that the VdPRMT1 Genes are associated with pathogenicity. The present invention further uses Verticillium dahliae VdPRMT1 As the target gene, the combination of transgenic and RNAi technologies is used to screen and obtain target gene fragments that can significantly improve plant disease resistance. The target gene fragment screened by the present invention and the Gateway interference vector constructed by using the target gene fragment can be applied to improving plant resistance to diseases caused by Verticillium dahliae and cultivating new varieties of transgenic plants resistant to Verticillium dahliae etc.

Description

technical field [0001] The present invention relates to Verticillium dahlia protein arginine methyltransferase 1 gene ( VdPRMT1 ), especially involving Verticillium dahliae VdPRMT1 Use of genes for improving resistance of crops or vegetables to pathogenic hazards belonging to Verticillium dahliae VdPRMT1 New fields of use for genes. Background technique [0002] Verticillium Wilt of cotton is called "cotton cancer". In many countries, cotton production has been reduced by an average of 10-35%, which has seriously endangered cotton production and caused huge economic losses (Wang Xiaokun; Wang Chunyan; Xie Chengjian; Yang Xingyong, Advances in molecular mechanisms of Verticillium dahliae pathogenicity and plant resistance to Verticillium dahliae. Henan Agricultural Science . 2014, 43 (01), 1-6.). The pathogen is Verticillium dahliae ( Verticillium dahliae ), it has a strong pathogenicity, and it can infect cotton throughout the growth stage, causing wilting and yellow...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/54C12N15/113C12N15/82A01H5/00A01H6/82A01H6/60A01H6/34A01H6/54
CPCC12N9/1007C12N15/1137C12N15/8218C12N15/8282C12Y201/01023C12N2310/14
Inventor 苏晓峰吴思源刘璐王琦程红梅
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI