Preparation of glycolipid type ionic liquid and application of glycolipid type ionic liquid in glycolipid separation process

A technology of ionic liquid and glycolipid, which is applied in the field of preparation of glycolipid-type ionic liquid, can solve the problems of complicated separation process, high production cost of rhamnolipid, and difficult separation of glycolipid fermentation liquid, etc. The process is green and environmentally friendly, and the structure is novel

Active Publication Date: 2021-11-05
WANHUA CHEM (SICHUAN) CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to provide a glycolipid ionic liquid, which solves the problems in the prior art that the separation of glycolipid fermentation liquid is difficult, the separation process is complicated, and the production cost of rhamnolipid is high.

Method used

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  • Preparation of glycolipid type ionic liquid and application of glycolipid type ionic liquid in glycolipid separation process
  • Preparation of glycolipid type ionic liquid and application of glycolipid type ionic liquid in glycolipid separation process
  • Preparation of glycolipid type ionic liquid and application of glycolipid type ionic liquid in glycolipid separation process

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Embodiment 2

[0055] The preparation process of di-n-butylamine rhamnolipid ionic liquid in this embodiment comprises the following steps:

[0056](1) Use a 20nm ceramic membrane to filter and separate 10kg of rhamnolipid fermentation broth to obtain 7.5kg of sterilized filtrate, add 7.5g of concentrated sulfuric acid to the filtrate, stir for 1 hour and precipitate out after stirring for 2 hours. Filter with a 20 μm nylon filter cloth, and collect a total of 503 g of solids.

[0057] (2) Mix the obtained solid with an equal mass of di-n-butylamine and transfer it to a three-necked flask, heat it to 50° C. for 1 hour under stirring, then cool it down to room temperature, add 200 g of dichloromethane, and transfer it to a separatory funnel after fully mixing. Set phase separation, separate the upper layer, a total of 857g, is a glycolipid-type ionic liquid. The structure is as follows

[0058]

Embodiment 2

[0060] The preparation process of di-n-octylamine glycolipid type ionic liquid in this embodiment comprises the following steps:

[0061] (1) Use a 500nm ceramic membrane to filter and separate 5kg of rhamnolipid fermentation liquid to obtain 4.5kg of sterilized filtrate, add 45g of concentrated sulfuric acid to the filtrate, stir for 3 hours and precipitate out after stirring for 1.5 hours. Filtration was carried out with a 50 μm polypropylene filter cloth, and a total of 305 g of solids were collected.

[0062] (2) Mix the obtained solid with an equal mass of di-n-octylamine and transfer it to a three-necked flask, heat it to 80° C. for 2 hours under stirring, then cool it down to room temperature, add 203 g of dichloromethane, and transfer it to a separatory funnel after fully mixing. Set phase separation, separate the upper layer, a total of 522g, is a glycolipid-type ionic liquid. The structure is as follows

[0063]

Embodiment 3

[0065] The preparation process of dicyclohexylamine glycolipid type ionic liquid in this embodiment comprises the following steps:

[0066] (1) Use a 200nm ceramic membrane to filter and separate 3kg of rhamnolipid fermentation liquid to obtain 2.8kg of sterilized filtrate, add 14g of concentrated sulfuric acid to the filtrate, stir for 2 hours and precipitate out after stirring for 1 hour. Filtration was carried out with a 30 μm polydimethylsulfoxide filter cloth, and a total of 209 g of solids were collected.

[0067] (2) Mix the obtained solid with an equal mass of dicyclohexylamine and transfer it to a three-necked flask, heat it to 60°C under stirring and keep it for 1.5 hours, then cool it down to room temperature, add 105g of dichloromethane, and transfer it to a separatory funnel after fully mixing. Set phase separation, separate the upper layer, a total of 389g, is a glycolipid-type ionic liquid. The structure is as follows

[0068]

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Abstract

The invention provides glycolipid type ionic liquid prepared by using rhamnolipid fermentation liquor and a preparation process and application of the glycolipid type ionic liquid. The preparation process comprises the following steps of: filtering the rhamnolipid fermentation liquor to obtain rhamnolipid sterile liquid; adding acid into the sterile liquid, and collecting precipitate to obtain solid rhamnolipid; and mixing the rhamnolipid with a dialkyl amine compound, adding a solvent, standing for layering, and separating to obtain the rhamnolipid ionic liquid. The ionic liquid provided by the invention is novel in structure, has high solubility and stability to glycolipid besides chemical inertness, high boiling point and good electrical conductivity of the ionic liquid, and is immiscible with water, so that the separation of the glycolipid from the fermentation liquor becomes simpler, and the difficulty of separation and purification of the glycolipid is greatly reduced.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, and in particular relates to the preparation of a glycolipid-type ionic liquid and its application in a glycolipid separation process. Background technique [0002] Rhamnolipid is mainly metabolized by Pseudomonas aeruginosa in a certain environment. It has good surface activity and interfacial activity and can be widely used in petrochemical, environmental, pharmaceutical, food and agricultural fields; it is non-toxic , The advantage of being biodegradable is a biosurfactant with the longest research time, the best effect and the most mature application technology. [0003] Glycolipid fermentation broth, especially rhamnolipid fermentation broth, contains impurities such as fermented bacteria, protein, and inorganic salts in addition to rhamnolipids. Generally, the crude product of rhamnolipids is obtained through centrifugal sterilization and acid precipitation, and then High-purity rha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/04C07H1/00C07C211/07C07C211/35C07C209/00C07H1/06
CPCC07H15/04C07H1/00C07C211/07C07C211/35C07C209/00C07H1/06C07C2601/14Y02P20/54
Inventor 石森乔小飞胡展曾雄伟李盼王峤潘亚男孙烨
Owner WANHUA CHEM (SICHUAN) CO LTD
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