Polysaccharide concentration detection method
A detection method, polysaccharide technology, applied in the field of inspection and detection, can solve the problems of complicated operation, time-consuming and labor-consuming, etc., and achieve the effect of simplifying the difficulty and making the measurement simple and convenient
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Embodiment 1
[0054] (1) The six different amounts of water soluble starch were added to the same amount of water, and the mixture was thored, and the concentration of six polysaccharides was 200 mg / ml, 100 mg / ml, 50 mg / ml, 10 mg / ml, 1 mg / ml, respectively. 0.1 mg / ml of polysaccharide standard solution;
[0055] (2) TDS values of each of the polysaccharide standard solutions are determined;
[0056] (3) The polysaccharide concentration in the standard solution of the water-soluble starch polysaccharide is the abscissa (X), the TDS value (Y) of the polysaccharide standard solution is a longitudinal coordinate, and the water-soluble starch polysaccharide concentration standard curve is drawn, and linear regression analysis, as a result, figure 1 As shown in the range of 0.1 mg / ml ~ 200 mg / ml, the standard curve: y = 3.6643x + 0.9685, R 2 = 0.9999, linear is better.
[0057] 1, accuracy measurement
[0058] The formulated concentrations were 120 mg / ml, 100 mg / mL, 80 mg / ml of...
Embodiment 2
[0079] Corporal chic slices of 50ml, DEAE cellulose column chromatography, column volume of 3.5 × 14.5 cm, pure water atmospheric pressure elution, flow rate of 2.5 ml / min, collected every 4 minutes. The TDS value of each tube was determined by the measurement method of the polysaccharide concentration proposed in the present invention. figure 2 , Use phenol-concentrated sulfuric acid method at 480 nm wavelengths to measure the absorbance A, obtained image 3 The polysaccharide concentration in the art is a common method in the art, and details are not described in this field.
[0080] Compare figure 2 with image 3 It can be seen that the change trend of the two figures is exactly consistent, so the method of determination of the polysaccharide concentration proposed by the present invention, and can be convenient and smooth and non-loss to obtain a phenol-concentrated land. The same result as the sulfuric acid method. The results obtained by the two methods were all incorporate ...
Embodiment 3
[0082] (1) The six different amounts of maltodextrin are added to the same amount of water, and the mixture is thoroughly dissolved, and the concentration of six polysaccharides is 200 mg / ml, 100 mg / ml, 50 mg / ml, 10 mg / ml, 1 mg / ml, respectively. 0.1 mg / ml of maltodecellular polysaccharide standard solution;
[0083] (2) TDS values of each of the polysaccharide standard solutions are determined;
[0084] (3) The polysaccharide concentration of the maltodecellular polysaccharide standard is the abscissa (x), the TDS value (Y) of the polysaccharide standard solution is a longitudinal coordinate, and the machine deduction polysaccharide concentration standard curve is drawn, and linear regression analysis, the result is Figure 4 As shown in the range of 0.1 mg / ml ~ 200 mg / ml, the standard curve: y = 1.4262X + 6.6676, R 2 = 0.9991, linear is better.
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