Preparation method and application of lipopolysaccharide composite nanoparticles
A polysaccharide compound and nanoparticle technology, applied in the field of biomedicine, can solve problems such as toxic side effects and normal tissue damage, and achieve the effect of easy operation, simple operation and high feasibility
Active Publication Date: 2021-11-12
CHONGQING MEDICAL UNIVERSITY
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Problems solved by technology
However, intravenous LPS often causes systemic toxic side effects, such as fever, chills, hypotension, and liver toxicity.
Other methods of administration, such as subcutaneous injection, intratumoral injection, and peritumoral injection, etc., can reduce systemic toxic reactions, but it is difficult for LPS to penetrate tumor tissue and play an effective role, and large doses of local injection can cause damage to normal tissues. damage
Method used
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Embodiment 1
[0041] In the present embodiment, the preparation method of lipopolysaccharide composite nanoparticles comprises the following steps:
[0042] 1) Dissolve 10mg of PLGA in advance in 1.5ml of dichloromethane, and put it in a 4°C refrigerator for later use;
[0043] 2) Weigh 1.5 mg ICG and dissolve it in 500 μl (1 mg / ml) lipopolysaccharide aqueous solution, and put it in a 4°C refrigerator for later use;
[0044] 3) Slowly oxygenate the PFP for 5 minutes, take 200 μl of oxygen-containing PFP and vibrate with the solution obtained in step 2) for 30 seconds, and then oxygenate it for 5 minutes;
[0045] 4) Mix the mixed solution obtained in step 1) with the emulsion obtained in step 3), emulsify for 3 minutes using an ultrasonic vibrator, pour into 5 mL of PVA aqueous solution (1% w / v), and vibrate for 3 minutes, and then Add 10 mL of isopropanol aqueous solution (2% v / v), and stir with magnetic force for 4 hours to fully volatilize the dichloromethane, and finally perform high-s...
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Abstract
The invention discloses a preparation method and application of lipopolysaccharide composite nanoparticles, and belongs to the technical field of biomedicine. The lipopolysaccharide composite nanoparticles have a stable outer membrane, the outer membrane is prepared from a polylactic acid-glycolic acid copolymer (PLGA), and a mixed solution consisting of liquid fluorocarbon (PFP), indocyanine green (ICG), lipopolysaccharide (LPS) and water is encapsulated in the nanoparticles. In the composite nanoparticles, the loading capacity of the indocyanine green is 10.18% + / -0.12%, and the loading capacity of the lipopolysaccharide is 1.69% + / -0.03%. According to the lipopolysaccharide composite nanoparticles, an immunologic adjuvant namely the LPS, a photosensitizer namely the ICG, oxygen and an oxygen-carrying phase change material namely the PFP are added on the basis of the PLGA nanoparticles, so that the stability and half-life period of the ICG in the circulation process are improved, the nanoparticles have good bimodal imaging capacity, and the lipopolysaccharide composite nanoparticles can be combined with photoacoustic power to induce the generation of immune response of a body; and the immune system is activated together with the LPS, so that the immunopotentiator has popularization and application values in the aspect of tumor immunotherapy.
Description
technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method and application of lipopolysaccharide composite nanoparticles. Background technique [0002] In the past decade, the integration of diagnosis and treatment has become a booming field of cancer treatment research, which has brought diagnosis and treatment closer together and made the treatment plan more individualized. Phototherapy (such as photothermal therapy and photodynamic therapy) has shown great advantages in emerging tumor treatments due to its advantages of less invasiveness, less side effects, and easy control. Sonodynamic therapy (SDT) is a new tumor treatment method developed on the basis of photodynamic therapy (PDT). Unlike PDT, PDT has poor tissue penetration, and ultrasound can penetrate deep into the tissue and target tumor tissue to mediate the cytotoxicity of ultrasound sensitizers. Photoacoustic combined therapy (Sono-Photodynamic the...
Claims
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IPC IPC(8): A61K41/00A61K9/51A61K47/34A61K49/22A61P35/00A61K39/39A61K39/00
CPCA61K41/0057A61K9/5153A61K49/22A61P35/00A61K39/39A61K39/0011A61K2039/55572Y02A50/30
Inventor 赵光宗常淑芳
Owner CHONGQING MEDICAL UNIVERSITY