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Method for purifying myricetin from myrica rubra leaves

A technology of myricetin and chlorophyll, applied in the direction of organic chemistry, can solve problems such as chlorophyll interference, and achieve the effects of simple operation, high yield and high purity

Active Publication Date: 2021-11-12
浙江大学中原研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a method for purifying myricetin from red bayberry leaves. The method aims at the problem that the separation and purification of myricetin from plant leaves (especially red bayberry leaves) is easily interfered by chlorophyll, and uses chlorophyllase to enzymatically hydrolyze Exclude chlorophyll interference, further combined with macroporous resin separation and purification to obtain high-purity (purity ≥ 95%) myricetin

Method used

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  • Method for purifying myricetin from myrica rubra leaves
  • Method for purifying myricetin from myrica rubra leaves
  • Method for purifying myricetin from myrica rubra leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Embodiment 1, a kind of method for purifying myricetin from red bayberry leaf, carries out following steps successively:

[0048] (1) Extraction (leaching):

[0049] Pass through a 100-mesh mesh sieve after crushing red bayberry leaves, weigh 1.0kg red bayberry leaf powder, add 5.0kg ethanol, leach at room temperature for 1 hour, and then filter with a 500-mesh filter to obtain extract I and filter residue respectively;

[0050] Replacing red bayberry leaves with filter residue, repeating the above leaching to obtain extract II, and combining the extracts (extract I, extract II) obtained from the two extractions to obtain an ethanol extract;

[0051] The ethanol extract contains chlorophyll, myricetin, myricetin glycoside and the like.

[0052] (2) Chlorophyll decomposition:

[0053] Add 0.25 kg of distilled water and an appropriate amount (about 40 g) of chlorophyllase to all the ethanol extracts obtained in step (1) in sequence, so that the enzyme content in the extra...

Embodiment 2

[0072] Example 2. Change the macroporous resin D101 in Example 1 into AB-8 type and HPD500 type macroporous resin, and the rest are the same as in Example 1.

[0073] The comparison of the yield and purity obtained in the above case with that of Example 1 is described in Table 1 below.

[0074] Table 1

[0075]

[0076]

Embodiment 3

[0080] Embodiment 3, change the concentrated hydrochloric acid in embodiment 1 step (3) into formic acid, and change its consumption accordingly, the pH of system is about 2. The rest are equal to Example 1.

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Abstract

The invention discloses a method for purifying myricetin from myrica rubra leaves, which comprises the following steps: crushing myrica rubra leaves, and conducting extracting with ethanol to obtain an ethanol extracting solution which contains chlorophyll, myricetin and myricetin glucoside; adding distilled water and chlorophyllase into the ethanol extracting solution, and then carrying out enzymolysis so as to decompose chlorophyll; adding an acidic auxiliary agent into the enzymatic hydrolysate to carry out acidification reaction, so as to convert myricetin glycoside into myricetin; and carrying out rotary evaporation on the extracting solution subjected to acid conversion to remove ethanol, adding distilled water, conducting standing, and purifying the obtained myricetin crude extract to obtain myricetin. According to the method, chlorophyll interference is eliminated by utilizing chlorophyll enzyme enzymolysis, and high-purity myricetin (the purity is greater than or equal to 95%) is obtained by further combining macroporous resin separation and purification.

Description

technical field [0001] The invention relates to a method for extracting and purifying myricetin from bayberry leaves. Background technique [0002] Myricetin, also known as myricetin, myricetin, myricetin, its chemical name is 5,7-trihydroxy-2-(3,4,5-trihydroxyphenyl)-4H-1-benzofuran- 4-keto, formula C1 5 h 10 o 8 , molecular weight 318.24. Myricetin is insoluble in water, but easily soluble in organic solvents such as methanol, ethanol, and acetone. A large number of studies at home and abroad have shown that myricetin has various physiological effects such as anti-oxidation, hypoglycemia, liver protection, anti-inflammation, and whitening. The US FDA has approved the application of myricetin in the fields of cosmetics, food, health products and medicine. [0003] Myricetin widely exists in various plant leaves, but the content of myricetin in plant leaves is relatively low. According to literature reports, the content of leaves of plants such as vine tea, Snakedon g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/40C07D311/30
CPCC07D311/40C07D311/30
Inventor 潘海波叶兴乾陈士国程焕陈健乐王艺徐新雷钱子琪
Owner 浙江大学中原研究院