A kind of chitosan microsphere and preparation method thereof
A technology of chitosan microspheres and chitosan, which is applied in the preparation of microspheres and microcapsule preparations, can solve the problems of uneven particle size, harsh conditions, and difficulty in regulating the particle size and distribution of microspheres, achieving The effect of mild conditions, simple process and broad application prospects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0029] The invention provides a preparation method of chitosan microspheres, comprising the following steps:
[0030] a) dissolving the modified chitosan in an organic solvent, and then adding a crosslinking agent to obtain a modified chitosan oil solution; then adding the modified chitosan oil solution to an emulsifier aqueous solution for pre-emulsification, to obtain an oil-in-water pre-emulsion;
[0031] b) subjecting the oil-in-water pre-emulsion obtained in step a) to rapid membrane emulsification, the obtained emulsion is then stirred and then centrifuged to collect the precipitate; finally, the obtained precipitate is washed with water and freeze-dried in sequence to obtain chitosan microspheres; The membrane used in the rapid membrane emulsification is an SPG membrane, and the pore size is 1 μm˜9 μm.
[0032] In the present invention, the modified chitosan is first dissolved in an organic solvent, and then a cross-linking agent is added to obtain a modified chitosan ...
Embodiment 1
[0063] Preparation of modified chitosan microspheres:
[0064] (1) Dissolve carboxymethyl chitosan in methylene chloride, make a solution of 50mg / ml, add sodium trimetaphosphate with 25% of carboxymethyl chitosan mass; under stirring speed of 1800r / min , adding the above solution into a 2% polyvinyl alcohol aqueous solution with a mass concentration of 50 times the volume, and stirring for 1 minute to obtain a pre-emulsion.
[0065] (2) Pour the pre-emulsion obtained in step (1) into a rapid membrane emulsification device, and pass the membrane 5 times under 0.2MPa inert gas pressure; the obtained emulsion is stirred at room temperature for 10h at a speed of 360r / min; the product is then collected by centrifugation, After the product was washed several times with water, it was freeze-dried to obtain microspheres with an average particle size of 500 nm.
[0066] The scanning electron microscope image of the chitosan microspheres obtained by the preparation method provided in E...
Embodiment 2
[0068] Preparation of modified chitosan microspheres:
[0069] (1) Dissolve hydroxypropyl chitosan in ethyl acetate, make a solution of 100mg / ml, add sodium trimetaphosphate with 25% mass of carboxymethyl chitosan; under stirring speed of 800r / min , adding the above solution into a 5% polyvinyl alcohol aqueous solution with a mass concentration of 10 times the volume, and stirring for 1 minute to obtain a pre-emulsion.
[0070] (2) Pour the pre-emulsion obtained in step (1) into a rapid membrane emulsification device, and pass the membrane 5 times under 0.2MPa inert gas pressure; the obtained emulsion is stirred at room temperature for 10h at a speed of 400r / min; the product is collected by centrifugation, After the product was washed several times with water, it was freeze-dried to obtain microspheres with an average particle size of 1 μm.
[0071] The scanning electron microscope image of the chitosan microspheres obtained by the preparation method provided in Example 2 of ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| pore size | aaaaa | aaaaa |
| concentration | aaaaa | aaaaa |
| particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More