Colloidal gold immunochromatography test strip for detecting benzoic acid in liquid food
A technology of immunochromatography test paper and liquid food, which is applied in the field of immunology, can solve problems such as damage to human health, and achieve the effect of high sensitivity
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Embodiment 1
[0022] Embodiment 1, the assembly of benzoic acid colloidal gold test strip
[0023] 1.1 Preparation of solution
[0024] Sample pad treatment solution: Accurately weigh 2 g of bovine serum albumin, 0.2 mL of Tween-20, 10 g of sucrose, and 3000.04 mL of proclin, dissolve in phosphate buffered saline (PBS, 10 mmol / L, pH 7.4) and set the volume to 200 mL.
[0025] Carbonate buffer (CB, 0.05mol / L, pH 9.6): Accurately weigh Na 2 CO 3 1.59g, NaHCO 3 2.93g, dissolved in deionized water and adjusted to 1000mL.
[0026] Secondary antibody diluent: bovine serum albumin 1g, Proclin 300 0.02mL, dissolved in phosphate buffered saline (PBS) to 100mL.
[0027] 1.2 Handling of sample pads
[0028] After fully soaking the cut sample pad with the sample pad treatment solution, take it out and put it in a blower dryer, and dry it at 37°C for 12 hours for later use.
[0029] 1.3 Coating of quality control line and detection line
[0030] T line (detection line): the benzoic acid antigen ...
Embodiment 2
[0036] Preparation and identification of embodiment 2 colloidal gold
[0037] 2.1 Preparation of colloidal gold
[0038] 2.1.1 Preparation of colloidal gold glassware processing
[0039] The Erlenmeyer flask for preparing colloidal gold was first rinsed with tap water, then soaked in concentrated acid (potassium dichromate 100g, concentrated sulfuric acid 200mL, added distilled water to 1000mL) for 24 hours, washed 3-4 times with ultrapure water, and then placed in Dry in the oven for later use.
[0040] 2.1.2 Preparation of solution
[0041] Preparation of 1% chloroauric acid: Weigh 1g of chloroauric acid and add it into 100mL of ultrapure water, mix well, use 1.5mL centrifuge tubes to pack, each tube has 1 mL, and store in a 4°C refrigerator for later use.
[0042] Preparation of 1% trisodium citrate: Dissolve 1.14 g of trisodium citrate dihydrate in 100 mL of ultrapure water, filter with a filter membrane after dissolution, and seal for later use.
[0043] 2.1.3 Trisodi...
Embodiment example 3
[0053] Implementation Case 3 Construction of Colloidal Gold Lateral Flow Chromatography
[0054] 3.1 Preparation of solution
[0055] 0.1mol / L of K 2 CO 3 : Accurately weigh 0.138g K 2 CO 3 , add 6 mL of deionized water to dissolve and mix well, and dilute to 10 mL with ultrapure water.
[0056] Carbonate buffer (CB, 0.05mol / L, pH 9.6): Accurately weigh Na 2 CO 3 1.59g, NaHCO 3 2.93g, dissolved in deionized water and adjusted to 1000mL.
[0057] Phosphate buffered saline (PBS, 10mmol / L, pH 7.4): Accurately weigh 8g of NaCl, 0.2g of KCl, NaCl 2 HPO 4 12H 2 O 2.9g, KH 2 PO 4 Dissolve 0.2g in a beaker with deionized water and set the volume to 1000mL.
[0058] Sample dilution buffer (0.5% PBST): Measure 1000 mL of phosphate buffer, add Tween-200.5 mL, and vortex to mix.
[0059] Gold-labeled antibody resuspension: Accurately weigh 1g of bovine serum albumin, 0.02mL of Proclin 300, 400001g of glucose, and 0.05g of PEG 20000, dissolve in 0.05mol / L phosphate buffer ...
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