Supercharge Your Innovation With Domain-Expert AI Agents!

Medicine for treating Newcastle disease virus infection and application thereof

A Newcastle disease virus and drug technology, applied in gene therapy, antiviral agents, medical preparations containing active ingredients, etc., can solve the problems of low lncRNA abundance, few lncRNA discovery, undocumented lncRNA, etc., to promote the development of Newcastle disease The effect of virus replication

Pending Publication Date: 2021-12-03
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with protein-coding genes, lncRNA has lower abundance, higher tissue specificity, and lower conservation among species (see: Djebali S,, eU al:Landscape of UranscripUion in humancells.NaUure 2012,489:101- 108)
[0004] With the widespread application of next-generation sequencing technology, lncRNA has gradually attracted people's attention and attention, and has been found to play an important role in disease occurrence, cell homeostasis, and cell metabolism. At present, some lncRNAs related to human diseases and viral infections have been discovered lncRNA, but the discovery of lncRNA associated with viruses infected by other species is still relatively small
[0005] To date, no lncRNAs associated with Newcastle disease virus infection have been documented in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Medicine for treating Newcastle disease virus infection and application thereof
  • Medicine for treating Newcastle disease virus infection and application thereof
  • Medicine for treating Newcastle disease virus infection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Analysis of expression levels of healthy cells and NDV infected cells lnc_000710 present embodiment.

[0050] Reference virulent Newcastle disease, respectively Herts / 33 reference attenuated NDV LaSota and infected chicken embryo fibroblast cell line DF-1 cells (ATCC, CRL-12203), 20 hours prior to virus infection, cells 1 × 10 ^ 6 / hole plated into 6 well plates in DMEM medium added with 10% FBS and placed in 37 ℃, 5% CO 2 Overnight culture conditions, viral infection, the medium was discarded, the cells were washed twice with PBS and the virus suspension was added 1mL 1MOI fresh medium, medium is replaced with fresh medium containing 1mL virus suspension, the cells were placed in 37 ℃, 5% CO 2 The incubator 1h, 1h after the virus-containing medium was discarded, replaced with DMEM medium containing 2% FBS in 37 ℃, 5% CO 2 The incubator 12h, uninfected chick embryo fibroblast line DF-1 cells (ATCC, CRL-12203) as a control, were extracted from virus infected cells and uni...

Embodiment 2

[0056] Cloning of the present embodiment lnc_000710 full-length sequences.

[0057] Extraction primary chicken embryo fibroblasts of the total RNA, according to RACE 5 ' / 3'Kit (ClontechLaboratories, Inc.)'S instructions 1μg total RNA was reverse transcribed using RACE primer (SEQ ID NO.3 ~ SEQ IDNO.6) for rapid amplification of cDNA ends, the specific PCR products were cloned and sequenced, and sequence alignments lnc_000710 obtain the full-length sequence lnc_000710 sequencing results figure 2 with image 3 Shown, lnc_000710 full length 4022bp, figure 2 Sequencing of the amplified product results 3'RACE display lnc_000710 with polyA tail.

Embodiment 3

[0059] Screening of the present embodiment of RNAi targets for lnc_000710.

[0060]The sequence of LNC_000710 obtained in Example 2 was sequenced, and the target of RNAi was designed, as shown in Table 1, SiRNA was synthesized by Shanghai Jima Pharmaceutical Technology Co., Ltd.. The DF-1 cell was inoculated with a 6-well plate, and the second day was about 40%, the SiRNA described in Table 1 was transfected with Lipofectamine 2000, and the transfection method refer to the Lipofectamine 2000 specification, and after transfection 48h, extract Cell total RNA, using RandomPrimer and M-MLV reverse transcriptase synthesis CDNAs, using Sybr Green Dye and Bio-Rad CFX96 Touch real-time PCR detection system to perform expression of LNC_000710 to transfection without functional RNA (siRNA-NC) DF-1 cells as controls, QPCR reaction conditions are as follows: 94 ° C, 53 ° C; 94 ° C, 15S, 59 ° C, 15S, 72 ° C, 15S, 40 cycles; 72 ° C extension 15s, primers in Example 1 The QPCR reaction is the sa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a medicine for treating Newcastle disease virus infection and application thereof. The medicine comprises a long-chain non-coding RNA (Ribonucleic Acid) lnc-000710, and a nucleotide sequence of the long-chain non-coding RNA lnc-000710 comprises a sequence as shown in SEQ ID NO. 1. The long-chain non-coding RNA lnc-000710 is found to be related to the replication of the Newcastle disease virus for the first time, the replication of the Newcastle disease virus can be promoted by inhibiting the expression of the lnc-000710, that is, the lnc-000710 can inhibit the replication of the Newcastle disease virus, and a new thought and a new method are provided for developing drugs for treating Newcastle disease virus infection and researching the replication of the Newcastle disease virus.

Description

Technical field [0001] The invention is within the field of biotechnology, involving a drug and its application thereof for treating new city disease virus infections. Background technique [0002] Newcastle disease (ND) is a viral infectious disease caused by the new city disease virus (NDV). It is one of the more severe poultry infectious diseases in today's world. It can cause systemic bleeding and gastrointestinal. Drat diseases, causing a lot of death in poultry, is one of the infectious diseases that endanger the poultry industry. [0003] Long chain non-coded RNA (LONG NON-CODING RNA, LNCRNA) is a class of non-encoded RNA molecules that do not encoded in the inclusion of 200 nu, which lack or have an open reading coding box (ORF), LNCRNA from RNA polymerase. II or RNA polymerase III transcription is processed, with a 5 'hat, but does not necessarily with Poly (a) tail, which is much lower than the encoded protein gene. LNCRNA has a lower abundance, which has a higher tissu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/7088A61P31/14C12N15/113
CPCA61K31/7088A61P31/14C12N15/1131C12N2310/14C12N2320/30
Inventor 刘炜玮丁铲孙英杰仇旭升宋翠萍谭磊廖瑛
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com