Black phosphorus wrapped with cell protein and preparation method of black phosphorus

Abstract

The invention relates to black phosphorus wrapped with cell protein and a preparation method of the black phosphorus, and belongs to a nano material surface modification technology. The preparation method comprises the following steps: (1) preparation of black phosphorus nanosheets and black phosphorus quantum dots: after grinding and ultrasonic treatment, carrying out liquid-phase stripping and gradient centrifugation to obtain well dispersed black phosphorus nanosheets with the particle size distribution of 50-300 nm and black phosphorus quantum dots with the particle size distribution of 4-20 nm; (2) preparation of cell protein: splitting the cells cultured in vitro by using a splitting solution, and performing centrifugal collection to obtain the cell protein; and (3) wrapping of the black phosphorus with the cell protein: mixing the cell protein with the black phosphorus nanosheets or the black phosphorus quantum dots, performing ultrasonic treatment, and then preparing the black phosphorus nanosheets with the surfaces wrapped with the cell protein by a co-extrusion method, wherein the black phosphorus nanosheets protect black phosphorus oxidation, slow down the degradation speed, are uniform in particle size distribution and good in biocompatibility, have targeted cells and has the surfaces wrapped with the cell protein. In order to prevent black phosphorus from being oxidized, the black phosphorus is endowed with target cell targeting, and the black phosphorus has wide application value in the fields of thermal therapy and photodynamic therapy in an implantation environment.

Description

technical field [0001] The invention belongs to nanomaterial surface modification and modification technology, relates to a black phosphorus wrapped by cell protein and a preparation method thereof, belongs to the field of biomedical engineering, and can be used for surface modification of biological materials. It can endow the implant with antioxidant properties, targeting and high biological activity, and promote the repair of the corresponding parts. Background technique [0002] Multifunctional two-dimensional (2D) materials have been explored and used in various fields of biomedicine, in which black phosphorus (BP) nanosheets (BP) S ) is an emerging two-dimensional material. In monolayer BP, each P atom is covalently bonded to three adjacent P atoms, resulting in a bilayer structure along the zigzag direction and a wrinkled structure along the armchair direction. The anisotropy of this structure endows it with special properties, including optical properties, mechanic...

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Problems solved by technology

However, these coatings will reduce the biological activity (near-infrared, bactericidal, etc.) and necessary degradability of black phosphorus nanosheets, and even affect the performance of black phosphorus in tumor treatment and tissue repair.

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