Collagen-PEG self-assembled sustained-release system as well as preparation method and application thereof

A self-assembly and collagen technology, which is applied in the field of biomedical materials, can solve the problems of limiting the application of collagen sustained-release systems and discounting therapeutic effects, and achieves the effects of long release time, obvious effect, and simple preparation process

Pending Publication Date: 2021-12-03
SHANGHAI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these new blood vessels will gradually disappear in the later stage with the lack of nutritional factors again, which greatly reduces the th

Method used

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  • Collagen-PEG self-assembled sustained-release system as well as preparation method and application thereof
  • Collagen-PEG self-assembled sustained-release system as well as preparation method and application thereof
  • Collagen-PEG self-assembled sustained-release system as well as preparation method and application thereof

Examples

Experimental program
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Example Embodiment

[0041] Another aspect of the embodiment of the present invention provides a preparation method of a collagen-PEG self-assembled slow-release system, comprising: soaking the collagen base material in the first liquid phase system and the second liquid phase system for more than one time in order to form a collagen-PEG self-assembly slow-release system. PEG self-assembled slow-release system;

[0042] Any one of the first liquid phase system and the second liquid phase system comprises a first growth factor and a collagen molecule specifically bound to the first growth factor, and the other comprises a PEG derivative molecule and a collagen molecule combined with the first growth factor The second growth factor combined with PEG derivative molecules, the collagen base material is mainly composed of collagen molecules, the first growth factor is a growth factor with a collagen binding domain, and the source of the PEG derivative molecules includes carboxylated PEG molecules .

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Example Embodiment

[0090] Example 1 The preparation method of the collagen-PEG sustained-release system provided in this example includes:

[0091] (1) Mix the collagen solution and the CBD-bFGF solution according to the volume ratio of 100:1 to form the first liquid phase system;

[0092] (2) Mixing the PEG derivative molecular solution and the bFGF solution at a volume ratio of 100:1 to form a second liquid phase system;

[0093] (3) Immerse the collagen substrate material (LOCS) in the first liquid phase system. After 30 min, wash with acetate buffer solution with pH value 5-7 to remove unbound collagen molecules, CBD-bFGF molecules, etc.

[0094] (4) Immerse the sample treated in step (3) in the second liquid phase system. After 30 minutes, wash it with an acetate buffer solution with a pH value of 5-7 to remove unbound PEG derivative molecules, bFGF, etc., and obtain collagen- PEG sustained release system.

Example Embodiment

[0095] Example 2 The preparation method of the collagen-PEG sustained-release system provided in this example is basically the same as in Example 1, but also includes:

[0096] (5) Steps 3, 6, 9, 12, and 15 of (3)-(4) were repeated to obtain different layers of collagen-PEG self-assembled slow-release systems.

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Abstract

The invention discloses a collagen-PEG self-assembled sustained-release system as well as a preparation method and an application thereof. The preparation method of the collagen-PEG self-assembled sustained-release system comprises the following steps: providing a first liquid phase system at least comprising a first growth factor and collagen molecules specifically bound with the first growth factor, wherein the first growth factor is a growth factor with a collagen binding domain; providing a second liquid phase system comprising at least PEG derivative molecules and a second growth factor bound to the PEG derivative molecules, wherein the PEG derivative molecules come from carboxylated PEG molecules; and sequentially soaking a collagen bundle in the first liquid phase system and the second liquid phase system, so as to form the collagen-PEG self-assembled sustained-release system. The self-assembled sustained-release system provided by the invention is simple in preparation process, large in growth factor loading capacity, long in release time, high in biological activity and good in mechanical property, and has a wide application prospect in the field of drug sustained release.

Description

technical field [0001] The invention relates to a tissue engineering scaffold material, in particular to a collagen-PEG self-assembled slow-release system and its preparation method and application, belonging to the field of biomedical materials. Background technique [0002] Tissue engineering scaffolds can provide a three-dimensional environment for regeneration, and an appropriate drug delivery system combined with tissue engineering scaffolds can significantly accelerate the repair of tissue damage. After tissue or organ injury occurs, the microenvironment of the injured area is destroyed. Inhibitory factors such as the infiltration of a large number of inflammatory factors and the formation of scars will hinder the process of tissue damage repair. The new blood vessels will provide nutrients for the reconstruction of the microenvironment, and can effectively provide the material support needed for tissue repair. At present, in the field of tissue engineering, loading ...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/18A61L27/22A61L27/24A61L27/54
CPCA61L27/24A61L27/18A61L27/227A61L27/54A61L2300/414C08L89/00C08L71/02
Inventor 戴建武陈艳艳赵海涛
Owner SHANGHAI UNIV
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