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A kind of saponin fermented product and its application in preventing and controlling diseases and insect pests and promoting growth

A technology for preventing and controlling pests and fermented products, which is applied to the fermented products of saponins and its application fields of preventing and controlling pests and promoting growth, can solve the problems of frequent occurrence of diseases and lack of better biological control methods, and achieve easy degradation and safety for humans and animals Non-toxic, simple production process

Active Publication Date: 2022-08-02
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, edible fungi such as bamboo fungus have frequent diseases, and chemical pesticides are still the main control measures at present, and there is no better biological control method

Method used

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  • A kind of saponin fermented product and its application in preventing and controlling diseases and insect pests and promoting growth
  • A kind of saponin fermented product and its application in preventing and controlling diseases and insect pests and promoting growth
  • A kind of saponin fermented product and its application in preventing and controlling diseases and insect pests and promoting growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Selection of saponin fermented bacteria

[0042] Compatibility determination: Weigh the dried saponin pods, pulverize them into coarse powder, take 10 g of saponin powder, add sterile water to prepare solutions of 1 mg / mL, 2 mg / mL, and 5 mg / mL, respectively, and measure by the filter paper method Whether saponin has an inhibitory effect on the growth of fermented bacteria.

[0043] We tested a variety of fermenting bacteria, and the results showed that the saponin has no effect on Bacillus amyloliquefaciens HN11 (deposited in the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences, with the preservation number of CGMCC No. 8421), Bacillus subtilis HN09 (separated by this laboratory, the preservation number is CGMCC No. 7524), as well as Lactobacillus acidophilus, Aspergillus oryzae, Mucor racemosus, Streptococcus thermophilus, Bifidobacterium breve, Saccharomyces cerevisiae (the above strains are all from South China (prov...

Embodiment 2

[0044] Example 2: Preparation of saponin fermented product

[0045] 1. Culture of Bacillus amyloliquefaciens HN11:

[0046] (1) According to the conventional culture method of microorganisms, pick a small amount of Bacillus amyloliquefaciens HN11 strain stored in LB solid medium or glycerol centrifuge tube, inoculate on the surface of LB solid plate, cultivate at 37 ° C for 1 day, as the bacteria of large-scale fermentation culture species, ready to use;

[0047] (2) Take the bacterial species on the surface of the above-mentioned LB solid plate, inoculate it into a conical flask of sterile LB liquid medium, and cultivate at 37° C. and 200 rpm on a shaker for 2 days for subsequent use.

[0048] The LB solid medium used therein: 1% tryptone, 0.5% yeast extract, 1% NaCl, 1% agar, distilled water, pH adjusted to 7.0.

[0049] (3) preparation of saponin fermented product:

[0050] Preparation of saponin: take the dried saponin pods, pulverize it into coarse powder, and obtain sap...

Embodiment 3

[0052] Example 3: Preparation of saponin fermentation product

[0053] The preparation of Bacillus amyloliquefaciens HN11 bacterial liquid and the preparation of saponin are the same as in Example 1.

[0054] Dilute the HN11 bacterial solution to 10 8 CFU / mL, add saponin residue according to the inoculation volume ratio of 3%, stir evenly, and ferment. The fermentation conditions were as follows: (1) the temperature was kept at 40°C for 2 days, (2) the temperature was raised to 55°C at a rate of 1°C / h, and the temperature was maintained for 4 days, (3) the temperature was lowered to 40°C at a rate of 1°C / h, and the fermentation was completed. Wherein the fermentation process was stirred once every 2h, and the pH value of the obtained fermentation product was 4.0.

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Abstract

The invention belongs to the technical field of biological control, in particular to the application of saponin fermented product in preventing and controlling diseases and insect pests and promoting growth. The invention discloses a saponin fermented product, which is obtained by using saponin as a raw material and inoculated with fermenting bacteria for fermentation. The saponin fermented product has good antifeedant and poisoning activities against major pests such as snails, snails, snails, nematodes and diamondback moths; at the same time, it shows that it has good inhibitory activities against major pathogens such as penicillium, trichoderma, aspergillus and bacillus The fermented product of saponin can also promote the absorption of nutrients and the growth of bacteria in bamboo fungus. The use of saponin fermented products can reduce the use of chemical pesticides, ensure food safety, and promote economic benefits, and has wide application prospects in production practice.

Description

technical field [0001] The invention belongs to the technical field of biological control. Specifically, it relates to a saponin fermented product and its application in preventing and controlling diseases and insect pests and promoting growth. Background technique [0002] Gleditsia sinensis has the functions of opening the orifices, removing phlegm and relieving cough, killing insects, killing rodents, and preventing and controlling plant diseases. It is often used as soil pesticides in the folk. At present, the biological activities of saponin are concentrated in the anti-oxidation, anti-tumor, sterilization and other traditional Chinese medicine aspects of saponin thorn. Some scholars have studied the insecticidal activities of saponin extracts against armyworms, cabbage butterflies, and cinnabar mites, such as " Research on the insecticidal activity of saponin extract and its mode of action on armyworms, Li Yuqi, 2012", but there are few studies on the pesticide activi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/22A01N63/20A01N63/34A01N63/30A01N63/32A01N65/20A01P5/00A01P7/04A01P9/00A01P3/00A01P1/00A01P21/00A01G13/00C12P1/02C12P1/04C12R1/07C12R1/125C12R1/23C12R1/69C12R1/785C12R1/46C12R1/865C12R1/01
CPCA01N63/22A01N63/20A01N63/34A01N63/30A01N63/32A01N65/20A01N65/00C12P1/02A01G13/00C12P1/04
Inventor 徐汉虹张雪张学学胡鑫田永清孙郑李贤嘉
Owner SOUTH CHINA AGRI UNIV
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