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Process for preparing hypotoxic vaccine for vincs of young parakeet

A parakeet and attenuated vaccine technology, applied in the direction of virus antigen components, etc., can solve the problems of unsafe and strong carcinogenicity

Inactive Publication Date: 2004-02-11
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Professor Müller of the University of Munich in Germany and a poultry disease scientist in the United States found that BFDV-DNA is stable to formaldehyde and sensitive to Ethylenimine (ethyleneimine), and this reagent has strong carcinogenicity; and the flash point is 42 ℃, extremely unsafe

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0009] Embodiment steps are as follows:

[0010] 1. Preparation of chicken embryo fibroblasts: use 9-10 day-old chicken embryos, take out the embryo body under sterile conditions, homogenate; add 5% trypsin digestion solution with pH 7.5, mix well, and put it in a 37°C water bath for digestion After 40 minutes, take out the centrifuge and discard the digestion solution; count the number of cells under a light microscope to 1×10 7 / ml; divided into 100ml cell culture flasks, add 10ml of 10% calf serum M199 tissue culture medium, and culture at 37°C for 16-18 hours to form a monolayer.

[0011] 2. Inoculate chick embryo fibroblasts (single layer) with 5% attenuated parakeet virus, and culture at 38°C (37-39°C is acceptable); 24 hours after the cells appear pathological changes, the cell body becomes round and swollen 1. The nucleus is enlarged and the netting phenomenon appears; collect the virus fluid for 48-72 hours (select the collection with obvious cytopathic changes withi...

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Abstract

A process for preparing hypotoxic vaccine for treating the virus diseases of young parakeet includes such steps as inoculating the hypotoxic liquid (5-10%) of the virus of young parakeet to fibroblase cells of chick embryo, culturing at 37-39.5 deg.C, collecting the virus liquid from lesion cells, diluting the virus liquid with 0.9% N.S in ratio of 1:10, examining and packing and cold storing. Its advantages high immune protection rate (99.62-100%), high safety, long storage period, and low cost.

Description

Technical field: [0001] The invention relates to a preparation method of an attenuated vaccine for preventing parrot juvenile virus disease. Background technique: [0002] Parrot juvenile disease (also known as parakeet juvenile virus disease) is a high-mortality competitive infectious disease caused by parakeet juvenile virus, and parrot birds are more susceptible. Since the 1990s, it has been widely popular in many regions of the world, seriously hindering the development of parrot breeding industry and various cage birds. Yunmeng County, Hubei Province is one of the largest cage bird breeding bases in the country. An unprecedented large-scale death of parrot chicks occurred between 1994 and 1996, and the mortality rate exceeded 80%. It quickly spread to other parrot breeding areas and other provinces, causing serious damage. economic loss. [0003] So far, there is no relevant research report on the use of vaccines to prevent parrot juvenile disease at home and abroad. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/12
Inventor 李天宪陈绳亮冯峰夏韦赵林
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI