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Method for regulating and controlling motion behavior of bio-enzyme motor based on allosteric activation strategy

A biological enzyme and motor technology, which is applied in the fields of biological antibacterial, nanomedicine and marine antibacterial and antifouling, can solve the problems of difficult to precisely control the movement behavior and lack of biological enzyme motors, and achieve low cost, sensitive response and strong controllability Effect

Pending Publication Date: 2021-12-21
CHANGCHUN NORMAL UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some biological enzyme motors have been conceptually prepared and optimized, there is still a lack of in-depth analysis of the interaction between the catalytic process of biological enzymes and the movement behavior of micro-nano motors driven by biological enzymes at the molecular level, so it is difficult to precisely regulate biological enzyme motors. sports behavior

Method used

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  • Method for regulating and controlling motion behavior of bio-enzyme motor based on allosteric activation strategy
  • Method for regulating and controlling motion behavior of bio-enzyme motor based on allosteric activation strategy
  • Method for regulating and controlling motion behavior of bio-enzyme motor based on allosteric activation strategy

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specific Embodiment approach 1

[0021] Specific embodiment 1: This embodiment records a method for regulating the movement behavior of biological enzyme motors based on an allosteric activation strategy, and the steps of the method are as follows:

[0022] Step 1: Surface modification of MSNPs (mesoporous silica nanoparticles) with octyltriethoxysilane;

[0023] Step 2: immobilizing the biological enzyme on the surface of the modified MSNPs obtained in step 1 to obtain the biological enzyme motor;

[0024] Step 3: Just mix the natural small-molecule surfactant and the bio-enzyme motor solution evenly, and use the natural small-molecule surfactant to regulate the movement behavior of the bio-enzyme motor.

specific Embodiment approach 2

[0025] Specific embodiment two: a kind of method based on the allosteric activation strategy described in specific embodiment one to regulate the movement behavior of biological enzyme motor, in step one, the concrete operation that carries out surface modification to MSNPs is:

[0026] (1) Disperse the MSNPs in EtOH to prepare the MSNPs dispersion, and sonicate for 10-15 minutes;

[0027] (2) Octyltriethoxysilane is configured into an aqueous solution;

[0028] (3) The aqueous solution of (2) was added to the MSNPs solution of (1), placed on a rotary shaker at 40-50° C. and vigorously stirred for 20-24 hours, then centrifuged and dried. Specifically, several times of centrifugal cleaning can be carried out, the purpose is to clean and purify the product, and remove excess octyl triethoxysilane, which can be washed in ethanol for 2 to 3 times in sequence (3500 to 4500 rpm, 10 ~12 minutes / time) and washing in water 2~3 times (3500~4500 rpm, 10~12 minutes / time), ultrasonication...

specific Embodiment approach 10

[0040] Specific embodiment ten: a kind of method based on the allosteric activation strategy of the specific embodiment 1 or 9 to regulate the movement behavior of the biological enzyme motor, in step 3, the natural small molecule surfactant is lecithin, cephalin, phospholipid One of the polar phospholipids such as acylserine.

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Abstract

The invention discloses a method for regulating and controlling the motion behavior of a bio-enzyme motor based on an allosteric activation strategy, and belongs to the technical field of biological antibiosis, marine antifouling, biological medicine and micro-nano motor synthesis and regulation and control. Aiming at the current situation that the motion ability of an existing lipase is difficult to regulate and control, the invention provides a new method for regulating and controlling the motion behavior of a lipase motor. The method comprises the following steps: 1, modifying the surfaces of silicon dioxide nanoparticles by using different methods to enable the silicon dioxide nanoparticles to be hydrophilic and hydrophobic; 2, preparing the bio-enzyme motor; and 3, adding a natural surfactant to regulate and control the movement performance of the bio-enzyme motor. According to the method, the catalytic efficiency of the lipase is adjusted based on the allosteric activation strategy, so that the motion behavior of the micro-nano motor driven by the lipase is improved, and the method has the advantages of simplicity in operation, high reaction speed, high controllability, low cost and the like.

Description

technical field [0001] The invention belongs to the technical fields of biological antibacterial, nano-medicine and marine antibacterial and antifouling, and specifically relates to a method for regulating the movement behavior of biological enzyme motors based on an allosteric activation strategy. Background technique [0002] Micro-nano motors have excellent properties such as good controllability, high targeting, and strong diffusion ability, so they have broad application prospects in the fields of high-efficiency antibacterial, controllable drug transport and release, tumor diagnosis and treatment, and marine antibacterial and antifouling. However, how to efficiently drive micro-nano motors and effectively regulate their motion behavior is the focus of current research and a necessary condition for their application in the biomedical field. At present, there are three main driving methods for micro-nano motors: external field driving, living organism driving and biologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C01B33/18
CPCC12N11/14C12N9/20C01B33/18C12Y301/01003
Inventor 宋凯莫金钢孙源王磊
Owner CHANGCHUN NORMAL UNIVERSITY