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Collection method for living body collection of embryos in pig body at 1, 2, 4 and 8 cell stages

A technology of embryos and cells, which is applied in the field of pig embryo transfer, can solve the problems of low-efficiency transgenic pigs, unqualified embryo transfer technology, and failure to obtain it, and achieve the effects of large litter size, increased production efficiency, and high conception rate

Pending Publication Date: 2021-12-24
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some transgenic embryos are prepared through a very complicated process. Maybe the previous work is very good, but because the embryo transfer technology is not up to standard, the transgenic pigs cannot be obtained or obtained with low efficiency. This is not worth the loss.

Method used

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  • Collection method for living body collection of embryos in pig body at 1, 2, 4 and 8 cell stages
  • Collection method for living body collection of embryos in pig body at 1, 2, 4 and 8 cell stages
  • Collection method for living body collection of embryos in pig body at 1, 2, 4 and 8 cell stages

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Experimental program
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Effect test

Embodiment 1

[0078] see Figure 1-4 , the present invention provides a technical solution: a method for collecting 1, 2, 4, and 8-cell embryos in live pigs, which uses the difference in time and space to determine the developmental stage of the embryo and the approximate position in the body , mainly including the following aspects:

[0079] 1. Collect 1-cell embryos, the best time is 18-24 hours after artificial insemination;

[0080] 2. Collect 2-cell stage embryos, the best time is 26-32 hours after artificial insemination;

[0081] 3. Collect 4-cell stage embryos, the best time is 34-40 hours after artificial insemination;

[0082] 4. Collect 8-cell embryos, the best time is 42-48 hours after artificial insemination.

[0083] The method mainly includes the following processes:

[0084] (1) Preparatory work before embryo collection

[0085] 1. Equipment preparation

[0086] (1) ovum flushing device: the ovum flushing device developed by the present invention is as follows: figure...

Embodiment 2

[0150] see Figure 1-4 , the present invention provides a technical solution: a method for collecting 1, 2, 4, and 8-cell embryos in live pigs, which uses the difference in time and space to determine the developmental stage of the embryo and the approximate position in the body , mainly including the following aspects:

[0151] 1. Collect 1-cell embryos, the best time is 18-24 hours after artificial insemination;

[0152] 2. Collect 2-cell stage embryos, the best time is 26-32 hours after artificial insemination;

[0153] 3. Collect 4-cell stage embryos, the best time is 34-40 hours after artificial insemination;

[0154] 4. Collect 8-cell embryos, the best time is 42-48 hours after artificial insemination.

[0155] The method mainly includes the following processes:

[0156] (1) Preparatory work before embryo collection

[0157] 1. Equipment preparation

[0158] (1) ovum flushing device: the ovum flushing device developed by the present invention is as follows: figure...

Embodiment 3

[0220] see Figure 1-4 , the present invention provides a technical solution: a method for collecting 1, 2, 4, and 8-cell embryos in live pigs, which uses the difference in time and space to determine the developmental stage of the embryo and the approximate position in the body , mainly including the following aspects:

[0221] 1. Collect 1-cell embryos, the best time is 18-24 hours after artificial insemination;

[0222] 2. Collect 2-cell stage embryos, the best time is 26-32 hours after artificial insemination;

[0223] 3. Collect 4-cell stage embryos, the best time is 34-40 hours after artificial insemination;

[0224] 4. Collect 8-cell embryos, the best time is 42-48 hours after artificial insemination.

[0225] The method mainly includes the following processes:

[0226] (1) Preparatory work before embryo collection

[0227] 1. Equipment preparation

[0228] (1) ovum flushing device: the ovum flushing device for oviduct flushing ovum developed by the present invent...

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Abstract

The invention discloses a method for living body collection of embryos in a pig body at 1, 2, 4 and 8 cell stages. The method mainly comprises the following steps of sow oestrus identification, artificial semen deposition, operational embryo flushing, embryo picking, in-vitro embryo culture and the like. According to the method for living body collection of the embryos in the pig body at the 1, 2, 4 and 8 cell stages and special equipment, the embryos in different development stages can be obtained from the fallopian tube of a donor sow for research of embryo development, gene prokaryotic injection, chimera animal preparation and the like by the method, the problems that in-vitro fertilized embryos are poor in quality and low in transplantation pregnancy rate are effectively solved, and therefore the production efficiency of transgenic or gene editing pigs is improved.

Description

technical field [0001] The invention relates to the technical field of pig embryo transplantation, in particular to a method for collecting 1-, 2-, 4-, and 8-cell embryos in live pigs. Background technique [0002] The success of the pig embryo transfer test was first reported by Kvashickii in 1951. By the 1960s, the technology of surgical method for collecting and transferring embryos had been basically established. After the 1970s, North America and Europe began to apply embryo transfer technology to the introduction of foreign blood and international introduction of closed pig herds. The emergence of transgenic animal technology in the 1980s promoted the application and development of embryo transfer technology. Good embryo collection technology can obtain the maximum number of embryos from the reproductive tract of donor sows for transgenic operations; and efficient embryo transfer technology can ensure that recipient sows can obtain a higher conception rate and a highe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D19/04
CPCA61D19/04
Inventor 华再东郭帅朱喆顾浩陈帆毕延震任红艳张立苹肖红卫
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI