Microorganism enrichment culture device

Abstract

The invention relates to the technical field of microorganisms, in particular to a microorganism enrichment culture device which comprises a culture box, and the culture box comprises a preparation chamber and a culture chamber which are in through connection; a liquid adding pipe and a first air pipe penetrate through the top wall of the preparation chamber; a matrix replacement pipe, a domestication liquid pipe and a second air pipe are arranged on the top wall of the culture chamber in a penetrating manner; the first air pipe, the second air pipe, the liquid adding pipe and the matrix replacing pipe are jointly connected with the height adjusting part; the culture box is also connected with an exhaust pipe; a sliding part and a culture part are further arranged in the culture box, the culture part is arranged on the sliding part, the culture part comprises a reactor and a culture bottle, the culture bottle is located in the reactor, and the reactor is arranged at the top of the sliding part. The invention provides a culture box which is convenient for sample adding, culture, domestication and culture solution replacement. While the interference of infectious microbes is effectively inhibited, the enrichment culture of microorganisms is realized, and the contrast experiment research is facilitated; and gas screening or liquid screening can be carried out according to the vital activity characteristics of the target microorganisms.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a microorganism enrichment culture device. Background technique [0002] Enrichment culture refers to a culture method that starts from the mixed group of microorganisms and leads to a pure culture by increasing the proportion of the number of specific species; it is a kind of culture method that makes the proportion of a specific microorganism in the mixed group of microorganisms increase sharply; one of which can be used Selective media or culture conditions that promote the growth and reproduction of a specific microorganism, the second can be selective media or culture conditions that inhibit the growth and reproduction of other microorganisms, and the third can be a continuous culture method that makes the specific growth rate smaller The cells overflow the incubator, while the cells that are larger than the growth rate remain in the incubator. [0003] Therefore, it...

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Problems solved by technology

[0004] The enrichment culture in the prior art all uses a simple culture bottle, and the microorganisms in the culture bottle are cultivated under certain conditions; therefore, the enrichment culture method in the prior art requires a large number of manual and cumbersome operations, and in actual operation It is also necessary to pay special attention to operations such as sterilization and control conditions; firstly, improper operation often leads to bacterial contamination, or waste of raw materials; secondly, it is impossible to achieve the same control of sample addition conditions and domestication conditions, especially when conducting comparative experiments. The asynchrony of the conditions leads to obvious errors; again, the operation during the domestication culture process is more complicated, and the control of the screening conditions and the replacement of the nutrient solution are inconvenient for simple culture bottles, especially for the gas screening of target microorganisms. The gas cannot enter the culture solution, and at the same time, the screening gas escapes, so a culture device is needed to overcome the above-mentioned technical defects

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