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High-efficiency and low-toxicity self-assembled cationic nanoparticles as well as preparation method and application thereof

A high-efficiency, low-toxicity, nanoparticle technology, applied in the field of gene transfection, can solve problems affecting membrane integrity and cytotoxicity, and achieve the effects of improving permeability, improving transfection efficiency, and reducing cytotoxicity

Pending Publication Date: 2022-01-18
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current research focuses on the transfection efficiency of PEI, and because PEI can interact with proteins or phospholipids on the cell membrane structure to affect the integrity of the membrane, resulting in cytotoxicity

Method used

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  • High-efficiency and low-toxicity self-assembled cationic nanoparticles as well as preparation method and application thereof
  • High-efficiency and low-toxicity self-assembled cationic nanoparticles as well as preparation method and application thereof
  • High-efficiency and low-toxicity self-assembled cationic nanoparticles as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] The present embodiment provides a self-assembled cationic nanoparticle with high efficiency and low toxicity, which is composed of polyethyleneimine and ursodeoxycholic acid; the mass ratio of polyethyleneimine and ursodeoxycholic acid is 4:1 . The nano particle diameter of the transfection reagent is 50-300nm.

[0029] This embodiment also provides a method for preparing self-assembled cationic nanoparticles with high efficiency and low toxicity, comprising the following steps:

[0030] S1: Dissolve polyethyleneimine and ursodeoxycholic acid in the above mass ratio in 1ml DMSO solvent to prepare a premixed solution, and make polyethyleneimine and ursodeoxycholic acid self-assemble in the premixed solution to form a polymer substance, the concentration of polyethyleneimine in the premixed solution is always kept at 5mg / ml;

[0031] S2: Transfer the prepared premixed solution to a dialysis bag for dialysis treatment, using deionized water at 25°C for dialysis, change t...

Embodiment 2

[0034] This embodiment provides a self-assembled cationic nanoparticle with high efficiency and low toxicity, which is composed of polyethyleneimine and cholic acid; the mass ratio of polyethyleneimine to cholic acid is 1:4. The nano particle diameter of the transfection reagent is 50-300nm.

[0035] This embodiment also provides a method for preparing self-assembled cationic nanoparticles with high efficiency and low toxicity, comprising the following steps:

[0036] S1: Dissolve polyethyleneimine and cholic acid in the above mass ratio in 2ml of methanol solvent to prepare a premixed solution, so that polyethyleneimine and cholic acid self-assemble in the premixed solution to form a polymer, and in the premixed solution The concentration of polyethyleneimine is always kept at 4mg / ml;

[0037] S2: Transfer the prepared premixed solution to a dialysis bag for dialysis treatment, using deionized water at 20°C for dialysis, changing the water every 12 minutes for the first 4 ti...

Embodiment 3

[0039] This example provides a self-assembled cationic nanoparticle with high efficiency and low toxicity, which is composed of polyethyleneimine and hyodeoxycholic acid; the mass ratio of polyethyleneimine and hyodeoxycholic acid is 1:3. The nano particle diameter of the transfection reagent is 50-300nm.

[0040] This embodiment also provides a method for preparing self-assembled cationic nanoparticles with high efficiency and low toxicity, comprising the following steps:

[0041] S1: Dissolve polyethyleneimine and hyodeoxycholic acid in the above mass ratio in 1ml of acetonitrile solvent to prepare a premixed solution, so that polyethyleneimine and hyodeoxycholic acid self-assemble in the premixed solution to form a polymer, The concentration of polyethyleneimine in the premixed solution is always maintained at 6mg / ml;

[0042] S2: Transfer the prepared premixed solution to a dialysis bag for dialysis treatment, using deionized water at 22°C for dialysis, changing the water...

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Abstract

The invention provides high-efficiency and low-toxicity self-assembled cationic nanoparticles as well as a preparation method and application thereof, and relates to the technical field of gene transfection. The invention relates to the high-efficiency and low-toxicity self-assembled cationic nanoparticle. The nanoparticle is prepared from polyethyleneimine and cholic acid medicines. The invention also provides a preparation method of the high-efficiency and low-toxicity self-assembled cationic nanoparticles, and the preparation method comprises the following steps of S1, dissolving polyethyleneimine and cholic acid medicines in a solvent to prepare a premixed solution; and S2, transferring the prepared premixed solution into a dialysis bag for dialysis treatment, and preparing the self-assembled cationic nanoparticles. The invention also provides an application of the high-efficiency and low-toxicity self-assembled cationic nanoparticle. According to the preparation method, the self-assembled cationic nanoparticles which are relatively low in cytotoxicity and capable of effectively improving transfection efficiency can be prepared.

Description

technical field [0001] The invention relates to the technical field of gene transfection, in particular to a self-assembled cationic nanoparticle with high efficiency and low toxicity, its preparation method and application. Background technique [0002] Gene transfection is a technology that "transfers or transports nucleic acids with biological functions into cells and maintains their biological functions in cells". Gene transfection technology has been widely used in genome function research (gene expression regulation, gene function, signal transduction and drug screening research) and gene therapy research. At present, gene transfection mainly relies on gene vectors, which can be divided into viral vectors and non-viral vectors; non-viral vectors are non-infectious, have no carrier capacity limitation, have a wide range of material sources, controllable chemical structures, and are easy to prepare in large quantities. Viral vectors play an irreplaceable role in the tar...

Claims

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Application Information

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IPC IPC(8): C12N15/87B82Y5/00B82Y40/00
CPCC12N15/87B82Y5/00B82Y40/00
Inventor 周兴徐祥张成元李子黎袁玉川
Owner ARMY MEDICAL UNIV
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