Application of resveratrol or resveratrol derivative in inhibition of penicillium expansum
A technology of resveratrol and Penicillium expansum, which is applied in the application field of resveratrol or resveratrol derivatives in inhibiting Penicillium expanse, can solve the problems of environmental pollution, low safety, and high cost, and achieve Obvious inhibitory activity, convenient operation, and enhanced inhibitory effect
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Embodiment 1
[0041] The influence of embodiment 1 resveratrol and pterostilbene on the mycelia growth of Penicillium extensa
[0042] Take out the purchased and stored Penicillium expansica strains, use an inoculation loop to pick up the expansion mold powder and activate it by streaking on the PDA medium. Rinse and activate the cultured Penicillium expanse spores with water to obtain a suspension of Penicillium expanse spores, count with a hemocytometer, and adjust the concentration of the suspension to 1×10 with the prepared sterile water. 7 spores / mL. Take 10 parts of 50mL PDA culture medium and divide them into two groups. Add 500μL of resveratrol to the 5 parts of the culture medium in the first group at about 40°C, so that the final concentration of resveratrol C1 corresponds to 0.1, 0.2, 0.4, 0.8 and 1.6mmol / L, 500μL of pterostilbene were added to the 5 parts of medium in the second group, so that the final concentration of pterostilbene C2 was 0.1, 0.2, 0.4, 0.8 and 1.6mmol / L, Bo...
Embodiment 2
[0046] The influence of embodiment 2 resveratrol and pterostilbene on the spore germination of Penicillium extensa
[0047] Take 10 parts of 50mL PDA culture medium and divide them into two groups. Add 500 μL of resveratrol to the 5 parts of the medium in the first group at about 40°C, so that the final concentration of resveratrol C1 corresponds to 0.1, 0.2, 0.4, 0.8 and 1.6mmol / L; 500 μL of pterostilbene were added to the 5 culture media of the second group, so that the final concentration of pterostilbene C2 was 0.1, 0.2, 0.4, 0.8 and 1.6mmol / L;
[0048] Then apply the above-mentioned PDA medium containing different concentrations of resveratrol and pterostilbene on sterile glass slides, and draw 20 μL of 1×10 7 spores / mL Penicillium extensa spore suspension (prepared in Example 1) was added dropwise to the culture medium. After culturing at 28°C for 10 h, the germination of spores was observed with an optical microscope. The result is as image 3 and Figure 4 shown. ...
Embodiment 3
[0050] Example 3 Effects of Resveratrol and Pterostilbene on the Cell Wall Integrity of Penicillium extensa
[0051] First take 100 μL of 1×10 7 spores / mL Penicillium extensa spore suspension (prepared in Example 1), inoculated into the prepared sterilized PDB liquid medium and cultivated for 24h, then divided into multiple parts equally, and added resveratrol and Pterostilbene solution, so that the final concentration C1 of resveratrol and the final concentration C2 of pterostilbene were 0.1, 0.2, 0.4, 0.8 and 1.6mmol / L respectively, and a blank group was set simultaneously (that is, the CK group was 1× 10 7 spores / mL expanded Penicillium spore solution), only 1 mL of DMSO solution was added to the blank group. After culturing in a shaker for 12 hours, it was determined according to the requirements of the AKP alkaline phosphatase kit. The result is as Figure 5 and Figure 6 shown. Since the experimental data are expressed as mean ± standard deviation, Figure 5 and ...
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