Check patentability & draft patents in minutes with Patsnap Eureka AI!

Indel marker c142, primers and its application for identifying ammonia nitrogen tolerance traits in Portunus trituberculatus

A technology of portunus trituratus and tolerance, which is applied in the field of DNA molecular markers, can solve the problems of increased bait input, excess nitrogen source, etc., and achieves the effects of low cost, simple method and reduced breeding cost.

Active Publication Date: 2022-03-22
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the improvement of the degree of intensification of farming, the density of farming has been increasing, and the input of bait has been increasing, resulting in excess nitrogen sources in the breeding water.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Indel marker c142, primers and its application for identifying ammonia nitrogen tolerance traits in Portunus trituberculatus
  • Indel marker c142, primers and its application for identifying ammonia nitrogen tolerance traits in Portunus trituberculatus
  • Indel marker c142, primers and its application for identifying ammonia nitrogen tolerance traits in Portunus trituberculatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The portunus trituberculatus used in this embodiment all get from the Xiaying Multiplication Station of the Chinese Academy of Fishery Sciences, select 200 swimming crabs with a body weight of 50 ± 5g, good vitality and no scars, and place them in 4 indoor culture ponds (500cm × 300cm ×150cm) for 7 days. During the temporary breeding period, the water temperature was kept at 23 ± 1°C, the salinity was 30 ± 1, the pH was 8.0 ± 0.3, the ammonia nitrogen concentration was lower than 0.01 mg / L, the water depth was 20cm, and oxygen was supplied continuously. Replace about 1 / 2 of the filtered seawater at 8 am every day, and feed fresh miscellaneous fish in the afternoon, and the feeding amount is about 1 / 5 of the total weight of swimming crabs.

[0020] After the temporary breeding, 200 swimming crabs were randomly assigned to 4 indoor breeding ponds, and 50 crabs were placed in each breeding pond. The concentration of ammonia nitrogen in seawater in the pool has been ammoni...

Embodiment 2

[0022] 1. Screening of molecular markers related to ammonia nitrogen tolerance traits

[0023] 1. Library construction, sequencing and data analysis

[0024] The Genomic DNA Extraction Kit from Marine Animal Tissue was used to extract the genomic DNA through the silica matrix material adsorption column. The specific steps are:

[0025] (1) Take about 25 mg of individual muscle tissue of Portunus trituberculatus, put it into a centrifuge tube filled with 200 μl buffer GA, vortex for 15 s, add 20 μl Proteinase K (20 mg / ml) solution, vortex After swirling and mixing, place at 56°C until the tissue is completely dissolved, and briefly centrifuge to remove water droplets on the inner wall of the tube cap.

[0026] (2) Add 200 μl of buffer GB, mix thoroughly by inversion, place at 70°C for 10 min, until the solution becomes clear, add 200 μl of absolute ethanol, and mix by inversion.

[0027] (3) Add the solution and flocculent precipitate obtained in the previous step to an adso...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an InDel marker C142, a primer and its application for identifying ammonia nitrogen tolerance traits of Portunus trituratus. It belongs to the technical field of DNA molecular markers, and the molecular marker C142 is an InDel marker whose nucleotide sequence is shown in SEQ ID No. 1, and the deletion homozygous is an ammonia nitrogen tolerance genotype. The present invention also uses the molecular marker C142 to design a pair of amplification primers with effective nucleotide sequences as shown in SEQ ID No. 2 and SEQ ID No. 3. The molecular marker C142 and its amplification primers can identify and screen ammonia-nitrogen-resistant swimming crab individuals at the molecular level, and the method is simple, accurate and reliable, and can effectively speed up the breeding process of new varieties of ammonia-nitrogen-resistant swimming crabs.

Description

technical field [0001] The invention belongs to the technical field of DNA molecular markers, and in particular relates to an Indel marker C142 for identifying the ammonia nitrogen tolerance trait of Portunus trituberculatus, a primer and an application thereof. Background technique [0002] Portunus trituberculatus ( Portunus trituberculatus ) belongs to Arthropoda, Crustacea, Decapod, Portunidae, Portunidae, and is an important marine economic crab mainly distributed in Bohai Sea, Yellow Sea, and East China Sea. Because of its delicious meat taste and rich nutrition, it contains protein, vitamin A, B1, B12 and niacin, as well as calcium, phosphorus, iron and other elements, and is deeply loved by consumers at home and abroad. Since the 1990s, artificial breeding of Portunus trituberculatus has developed rapidly in my country, and by 2019, the annual production of swimming crabs has reached 113,800 tons. However, with the improvement of the degree of intensification of fa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 孟宪亮刘晓晨王黛霞高保全贾少婷刘萍李健
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com