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Composition of phellinus igniarius submerged fermentation concentrated solution and thallus extract as well as preparation method and application of composition

A technology of submerged fermentation and concentrated solution, which is applied in the direction of biochemical equipment and methods, drug combinations, and methods based on microorganisms, can solve the problems that the application value has not been fully explored, and achieve the effect of promoting collagen production and delaying skin aging

Pending Publication Date: 2022-02-08
TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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  • Claims
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AI Technical Summary

Problems solved by technology

Fermentation engineering is a production method that makes full use of secondary metabolites of fungi. At present, the application value of secondary metabolites of various lamellar fungi in the skin care industry has not been fully explored

Method used

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  • Composition of phellinus igniarius submerged fermentation concentrated solution and thallus extract as well as preparation method and application of composition
  • Composition of phellinus igniarius submerged fermentation concentrated solution and thallus extract as well as preparation method and application of composition
  • Composition of phellinus igniarius submerged fermentation concentrated solution and thallus extract as well as preparation method and application of composition

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preparation example Construction

[0037] One embodiment of the present invention proposes a method for preparing a high-temperature-induced submerged fermentation concentrate and bacterial cell extract composition, comprising the following steps:

[0038] 1) Inoculate the activated Phyloporus strain into the liquid culture medium, perform the first fermentation culture at 28° C., and obtain the first-grade liquid fermentation seeds;

[0039] 2) After the above-mentioned primary liquid fermented seeds are dispersed, they are inoculated into a new liquid medium for a second fermentation culture, and the temperature of the second fermentation culture is raised from 28°C to 30°C to obtain a second stage liquid fermented seeds;

[0040] 3) After the above-mentioned secondary liquid fermentation seeds are dispersed, they are inoculated into a new liquid medium for the third fermentation culture, and the temperature of the third fermentation culture is raised from 30°C to 32°C. After the fermentation, the third-stage ...

Embodiment 1

[0061] Example 1 The method for preparing the high-temperature-induced submerged fermentation concentrate of Phyloporus bacterium, the extract of the thalline, and the composition of the two (taking Phylofora hoofii as an example), comprises the following steps:

[0062] Step 1: Inoculate the activated Phyloporus spp. (Ph. hoofii) strain into the 2 PO 4 and CaCl 2 In the liquid culture medium, 28 ℃, 150r / min cultivated 7d, obtain the primary liquid fermentation seed.

[0063] Step 2: After the primary liquid fermented seeds are dispersed, they are transferred to a new liquid seed medium according to 10% inoculum size, the fermentation temperature is slowly increased from 28°C to 30°C, and cultured at 150r / min for 7 days to obtain secondary liquid fermented seeds.

[0064] Step 3: After the secondary liquid fermented seeds are dispersed, they are connected to a new test medium according to 10% inoculation amount, and the fermentation temperature is slowly increased from 30°...

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Abstract

The invention provides a composition of phellinus igniarius submerged fermentation concentrated solution and thallus extract as well as a preparation method and application thereof, and belongs to the technical field of biological skin care products. The method comprises the following steps: 1) preparing primary liquid fermentation seeds; 2) preparing secondary liquid fermentation seeds; (3) after the secondary liquid fermentation seeds are dispersed, inoculating the secondary liquid fermentation seeds into a new liquid culture medium for third fermentation culture, centrifuging a tertiary liquid fermentation product after fermentation is finished, and respectively collecting thalli and fermentation liquor of the thalli; (4) adding sterile ultrapure water into the thalli, suspending, adding a protective agent, homogenizing, carrying out thermal cracking, filtering, and collecting a thalli extracting solution; carrying out vacuum rotary evaporation and concentration to obtain a thallus extract; (5) filtering and degerming the fermentation liquor of the thalli, and carrying out vacuum rotary evaporation and concentration to obtain deep fermentation concentrated liquor; and 6) mixing the thallus extract and the submerged fermentation concentrated solution to obtain the composition. The obtained composition has the effects of resisting oxidation and inflammation, promoting collagen generation and delaying skin aging.

Description

technical field [0001] The invention belongs to the technical field of biological skin care products, and in particular relates to a composition of submerged fermented concentrated solution and bacterium extract of the fungi and its preparation method and application. Background technique [0002] Fungi are a class of eukaryotic organisms that are widely distributed on land and in water, relying on decomposing existing organic matter or absorbing nutrients from living animals and plants. The vast majority of fungal organisms in nature are composed of mycelium, which reproduce sexually or asexually by producing a large number of spores, which grow into mycelium under suitable conditions. Subsequently, these mycelia differentiate into fruiting bodies of different shapes, becoming the fungal-like organisms observed in nature. [0003] Fungi have extremely high nutritional value and have a long history of edible and medicinal value in our country. This is because bacteria cont...

Claims

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Application Information

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IPC IPC(8): A61K8/9728A61K8/9789A61K8/9767A61Q19/08A61P29/00C12N1/14C12R1/645
CPCC12N1/14A61K8/9728A61K8/9789A61K8/9767A61Q19/08A61P29/00A61K2800/85
Inventor 张冉冉范培浩杜玉兰张云贤杜丽玥孙茂杰
Owner TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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