Composition of phellinus igniarius submerged fermentation concentrated solution and thallus extract as well as preparation method and application of composition

A technology of submerged fermentation and concentrated solution, which is applied in the direction of biochemical equipment and methods, drug combinations, and methods based on microorganisms, can solve the problems that the application value has not been fully explored, and achieve the effect of promoting collagen production and delaying skin aging

Pending Publication Date: 2022-02-08
TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Fermentation engineering is a production method that makes full use of secondary metabolites of fungi. At present, the a

Method used

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  • Composition of phellinus igniarius submerged fermentation concentrated solution and thallus extract as well as preparation method and application of composition
  • Composition of phellinus igniarius submerged fermentation concentrated solution and thallus extract as well as preparation method and application of composition
  • Composition of phellinus igniarius submerged fermentation concentrated solution and thallus extract as well as preparation method and application of composition

Examples

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Example Embodiment

[0037] The present invention proposes a submerged fermentation embodiment concentrates with a high-temperature inducing cells prepared Phellinus extract composition, comprising the steps of:

[0038] 1) after activation Phellinus strain was inoculated into the liquid medium, a first fermentation 28 deg.] C to give a liquid fermentation seed;

[0039] 2) After the above seed dispersion a liquid fermentation, inoculated into fresh liquid culture medium, a second fermentation, fermentation temperature of the second raised from 28 ℃ 30 ℃, to obtain two liquid fermentation seed;

[0040] 3) After the above two liquid fermentation seed dispersion, inoculated into a new third fermentation broth, fermentation third temperature rose from 30 ℃ 32 ℃, after the end of the fermentation, the fermentation product of the liquid was centrifuged three , cells were collected, the cell broth;

[0041] 4) After adding sterile ultrapure water to the suspended cells, the protective agent was added, homo...

Example Embodiment

[0061] Example 1 High temperature induced laminate deep fermentation concentrate, bacteria extract, and the preparation method of the two compositions (as an example of hoof fragini, including the steps), including the following steps:

[0062] Step 1: Activated lactacles (hoovescetaclasion) strain inoculated into maltose, yeast, protein, kH 2 PO 4 CaCL 2 In a liquid medium, 28 ° C, 150 r / min culture 7d, resulting in a level of liquid fermentation seeds.

[0063] Step 2: After the primary liquid fermentation seed is dispersed, in the new liquid seed medium, the fermentation temperature is slowly increased from 28 ° C to 30 ° C, 150 r / min culture 7d, and the secondary liquid fermentation seed was slowly raised from 28 ° C.

[0064] Step 3: After the secondary liquid fermentation seed is dispersed, it is adjusted to a new test medium according to the amount of 10%, and the fermentation temperature is slowly raised from 30 ° C to 32 ° C, 150 r / min culture 7d, and the three-sta...

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Abstract

The invention provides a composition of phellinus igniarius submerged fermentation concentrated solution and thallus extract as well as a preparation method and application thereof, and belongs to the technical field of biological skin care products. The method comprises the following steps: 1) preparing primary liquid fermentation seeds; 2) preparing secondary liquid fermentation seeds; (3) after the secondary liquid fermentation seeds are dispersed, inoculating the secondary liquid fermentation seeds into a new liquid culture medium for third fermentation culture, centrifuging a tertiary liquid fermentation product after fermentation is finished, and respectively collecting thalli and fermentation liquor of the thalli; (4) adding sterile ultrapure water into the thalli, suspending, adding a protective agent, homogenizing, carrying out thermal cracking, filtering, and collecting a thalli extracting solution; carrying out vacuum rotary evaporation and concentration to obtain a thallus extract; (5) filtering and degerming the fermentation liquor of the thalli, and carrying out vacuum rotary evaporation and concentration to obtain deep fermentation concentrated liquor; and 6) mixing the thallus extract and the submerged fermentation concentrated solution to obtain the composition. The obtained composition has the effects of resisting oxidation and inflammation, promoting collagen generation and delaying skin aging.

Description

technical field [0001] The invention belongs to the technical field of biological skin care products, and in particular relates to a composition of submerged fermented concentrated solution and bacterium extract of the fungi and its preparation method and application. Background technique [0002] Fungi are a class of eukaryotic organisms that are widely distributed on land and in water, relying on decomposing existing organic matter or absorbing nutrients from living animals and plants. The vast majority of fungal organisms in nature are composed of mycelium, which reproduce sexually or asexually by producing a large number of spores, which grow into mycelium under suitable conditions. Subsequently, these mycelia differentiate into fruiting bodies of different shapes, becoming the fungal-like organisms observed in nature. [0003] Fungi have extremely high nutritional value and have a long history of edible and medicinal value in our country. This is because bacteria cont...

Claims

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Application Information

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IPC IPC(8): A61K8/9728A61K8/9789A61K8/9767A61Q19/08A61P29/00C12N1/14C12R1/645
CPCC12N1/14A61K8/9728A61K8/9789A61K8/9767A61Q19/08A61P29/00A61K2800/85
Inventor 张冉冉范培浩杜玉兰张云贤杜丽玥孙茂杰
Owner TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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