Method for enhancing bacterial resistance of plants
A plant, resistance technology for agronomy that addresses poorly understood pathway connections that limit defense and disease resistance
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Embodiment 1
[0268] Example 1 Ralstonia solanacearum infection enhances pyruvate decarboxylase activity in host plants
[0269] The invention measures the activity of pyruvate decarboxylase in Arabidopsis thaliana and tomato during Ralstonia solanacearum infection. Such as figure 1 ( figure 1 , A-B) showed that the activities of pyruvate decarboxylase in Arabidopsis and tomato plants were significantly enhanced 3 days after inoculation with R. solanacearum GMI1000. Notably, R. solanacearum rapidly depletes available oxygen in the xylem during active infection by the pathogen, creating a hypoxic environment. Thus, the present data demonstrate that rapid replication of R. solanacearum GMI1000 (but not the non-pathogenic mutant) and subsequent depletion of limited oxygen triggers plant responses to hypoxia in infected tissues, including pyruvate decarboxylase activity rapid increase.
Embodiment 2
[0270] Example 2 The role of PDCs in plant resistance to bacterial wilt
[0271] In order to determine the role of pyruvate decarboxylase gene in tomato bacterial wilt resistance, the present invention uses the RNAi vector expression system that can target silence tomato root, the gene SLPDC2( figure 2 A). After inoculation with R. solanacearum GMI1000, tomato plants appeared to be more susceptible to the disease ( figure 2 B and 2C). This shows that the PDC gene contributes to the resistance of tomato to bacterial wilt.
[0272] In order to determine the role of pyruvate decarboxylase gene in tomato bacterial wilt resistance, the present invention silences the corresponding PDC gene in two plant systems. On the one hand, the T-DNA insertion mutant strain pdc2 was used in Arabidopsis to silence the gene AtPDC2 of Arabidopsis ( figure 2 A). After inoculation with R. solanacearum GMI1000, Arabidopsis mutants were more susceptible to disease ( figure 2 B and 2C). It sh...
Embodiment 3
[0273] Example 3 The pathway mediated by pyruvate decarboxylase contributes to enhancing the disease resistance of tomato to Ralstonia solanacearum
[0274] In order to determine whether the acetate pathway mediated by pyruvate decarboxylase enhances the disease resistance of tomato to R. solanacearum, the present invention uses exogenous pyruvate and acetate as the substrate and product of the pathway to pretreat the roots of tomato plants, respectively. Treatment was performed once a day by placing the planted soil on wet paper towels doused with organic acid for 9 days, after which the soil was washed to remove remaining acid and watered normally for 3 days without other treatment , and finally infect the plants with a certain concentration of R. solanacearum. The present invention finds that the pretreatment of pyruvate and acetic acid greatly enhances the disease resistance of tomato plants to Ralstonia solanacearum, showing a sharp reduction and delay of bacterial wilt s...
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