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Sulfydryl codonopsis pilosula polysaccharide and application thereof in colon-targeted probiotic microcapsule preparation

A technology of mercapto Codonopsis pilosula and probiotics, which is applied in the direction of microcapsules, capsule delivery, medical preparations of non-active ingredients, etc., can solve the problem of weak ligand-receptor binding and reduce the abundance and content of intestinal pathogenic bacteria , the effect of reducing the degree of damage

Active Publication Date: 2022-02-18
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ligand-receptor binding is relatively weak, different strains have different binding abilities, and the resident flora in the gut also competes with ingested bacteria

Method used

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  • Sulfydryl codonopsis pilosula polysaccharide and application thereof in colon-targeted probiotic microcapsule preparation
  • Sulfydryl codonopsis pilosula polysaccharide and application thereof in colon-targeted probiotic microcapsule preparation
  • Sulfydryl codonopsis pilosula polysaccharide and application thereof in colon-targeted probiotic microcapsule preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The preparation method of embodiment 1 mercapto Codonopsis polysaccharide (sC-CPP-2)

[0063] 1. Preparation of carboxymethyl codonopsis polysaccharide (C-CPP-2)

[0064] Dissolve 1-2g Codonopsis polysaccharide (CPP-2) in 50-100mL deionized water, after fully dissolved, add 150-300mL NaOH solution (3mol / L), stir for 10min; add 20-40g monochloroacetic acid, react at 65°C 4h; cool to room temperature, adjust the pH value to 7.0, and perform dialysis until the remaining reagents are eliminated; vacuum-dry the product obtained by dialysis to obtain carboxymethylated Codonopsis polysaccharide (C-CPP-2);

[0065] 2. Preparation of thiol codonopsis polysaccharide (sC-CPP-2)

[0066] Dissolve 100-200mg carboxymethylated Codonopsis polysaccharide C-CPP-2 in 20-40mL distilled water, fully dissolve; then add 100-500mg 1-ethyl-(3-dimethylaminopropyl)carbodiethylene Amine hydrochloride (EDC), activate the carboxyl group in the reaction system, stir at room temperature for 1 h; add...

Embodiment 2

[0067] Structural characterization of embodiment 2 mercapto Codonopsis polysaccharide (sC-CPP-2)

[0068] 1. Determination of thiol content

[0069] The Ellman's method was used to determine the content of thiol in Codonopsis polysaccharide sC-CPP-2. Prepare 12.5, 25, 50, 100, 200 μmol / L L-cysteine ​​solutions; add 1mL L-cysteine ​​solution to a certain amount of DTNB solution, let stand for 10min, measure the absorbance at 412nm, and establish a standard curve.

[0070] Take 50-100mg of mercapto Codonopsis polysaccharide sC-CPP-2, dissolve it in 100-200ml of deionized water, take the sample solution and test it according to the above method, and use the standard curve to calculate the mercapto group content.

[0071] The results showed that the thiol content in the mercapto Codonopsis polysaccharide (sC-CPP-2) prepared above was 279.50±5.97 μmol / g.

[0072] 2. Potential change analysis

[0073] The zeta-potentials of CPP-2, C-CPP-2 and sC-CPP-2 were determined by potentio...

Embodiment 3

[0075] Example 3 Interaction relationship between mercapto Codonopsis polysaccharide (sC-CPP-2) and intestinal mucus

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Abstract

The invention relates to the technical field of probiotic application, in particular to sulfydryl codonopsis pilosula polysaccharide and application thereof in colon-targeted probiotic microcapsule preparation. The sulfydryl codonopsis pilosula polysaccharide can remarkably improve the adhesion effect of intestinal mucus on probiotics, and the effect is remarkable. The probiotic microcapsule prepared by combining the sulfydryl codonopsis pilosula polysaccharide and the probiotics can well protect the probiotics from avoiding the digestion effect of gastrointestinal tracts, the probiotics are delivered to the colon in a targeted mode and are completely released in the colon, the retention time of the probiotics in the colon can be remarkably prolonged, and then effective colonization of the probiotics in the colon is promoted, and the probiotic effect is achieved.

Description

technical field [0001] The invention relates to the technical field of probiotic application, in particular to a mercapto codonopsis polysaccharide and its application in the preparation of colon-targeted probiotic microcapsules. Background technique [0002] Probiotics, also known as microecological regulators and live bacterial preparations, can improve the health of the body by regulating the balance of intestinal microecology. When the intestinal microecology is out of balance, it is usually necessary to supplement exogenous probiotics to adjust the composition of intestinal flora and improve the disease. However, probiotics can only be effective if they exist in the gut for a long time and reach a certain number. But the reality is not what we hoped. Exogenous probiotics are generally difficult to resist the digestion of the gastrointestinal tract. Studies have shown that the survival rate of bifidobacteria after passing through the gastric juice environment is less t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61K35/741A61K9/50A61K47/36A61K47/32A61P1/00A61P1/04
CPCC08B37/0003A61K31/715A61K35/741A61K9/5036A61K9/5026A61K9/5073A61K47/36A61P1/00A61P1/04A61K2300/00Y02A50/30
Inventor 乌日娜武俊瑞李默杨慧史海粟
Owner SHENYANG AGRI UNIV