Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for purifying free amino acids in Ribes stenocarpum Maxim

A purification method, amino acid technology, applied in separation methods, chemical instruments and methods, ion exchange, etc., can solve problems such as affecting purity

Active Publication Date: 2022-02-25
QINGHAI UNIVERSITY
View PDF23 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been proved that free amino acids in plant resources have various physiological functions such as anti-tumor, lowering blood pressure and regulating intestinal flora [15-17], but due to the crude extracts of free amino acids extracted by different solvents It will be accompanied by the dissolution of other components, which will affect its purity, so it is necessary to purify the crude amino acid extract so that its biological activity can be analyzed more systematically and scientifically in the next step

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for purifying free amino acids in Ribes stenocarpum Maxim
  • Method for purifying free amino acids in Ribes stenocarpum Maxim
  • Method for purifying free amino acids in Ribes stenocarpum Maxim

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 Purification process research of the present invention

[0021] 1 Materials and methods

[0022] 1.1 Materials and reagents

[0023] Narrow fruit tea currant: Huzhu, Qinghai Province, collected at an altitude of 3029 meters, longitude 101° 50.926, latitude 36° 57.670, after picking, it was stored in a -20°C refrigerator for later use.

[0024] SA-2, 001*7, 201*7, D61, D152 type resin (batch number: HC210409, Zhengzhou Hecheng New Material Technology Co., Ltd.); absolute ethanol, ninhydrin, potassium dihydrogen phosphate, disodium hydrogen phosphate , ammonia water, hydrochloric acid (analytical grade, Tianjin Yongda Chemical Reagent).

[0025] 1.2 Instruments and equipment

[0026] YM-031S Ultrasonic Cleaner (Shenzhen Fuyang Technology Co., Ltd.); R-1001VN Rotary Evaporator (Zhengzhou Great Wall Technology Industry and Trade Co., Ltd.); SPH-304 New Decolorization Shaker (Shanghai Shiping Test Equipment Co., Ltd. ); HL-2B constant current pump (Shanghai ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for purifying free amino acids in Ribes stenocarpum Maxim. Ribes stenocarpum Maxim ethanol extract passes through ion exchange resin, wherein the concentration of a sample loading solution is 0.01-0.030 g / mL, the sample loading flow rate is 0.5-2.5 mL / min, the pH value of the sample loading solution is 3-7, the volume number of an ammonia water eluent is 0.5-2.5%, the elution flow rate is 0.5-2.5 mL / min, and the volume of the eluent is 30-70 mL. The research can provide technical support for purification of amino acid components of Ribes stenocarpum Maxim in Qinghai-Tibet Plateau.

Description

technical field [0001] The invention relates to the extraction of natural plant active ingredients. Background technique [0002] Narrow fruit tea currant (Ribes stenocarpum Maxim.) also known as long fruit gooseberry [1] , is a unique wild berry resource in the Qinghai-Tibet Plateau. The fruit of this genus is bright and oval, rich in juice, which contains rich biologically active ingredients, such as amino acids, vitamins, phenolic compounds, etc. [2] , has long been used by local residents as food to prevent colds, therefore, is considered to have high potential medicinal value. Studies have shown that increased intake of edible berries has a protective effect against several chronic diseases such as diabetes and high blood pressure [3-5] . The resources of tea currant in Qinghai-Tibet Plateau are abundant, most of which are in the wild state, and the research on its chemical constituents is still in its infancy. [0003] Amino acids are the material basis necessary ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/20B01D15/36B01D15/42
CPCB01D15/361B01D15/20B01D15/426
Inventor 叶英杨芳王虹刘哲石世薇
Owner QINGHAI UNIVERSITY