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A technology of fingerprint spectrum and detection method, applied in the field of Chinese patent medicine detection, can solve the problem of single detection index, and achieve the effects of good reproducibility, high precision and good stability
Pending Publication Date: 2022-03-01
NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +1
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[0004] However, at present, the content determination of the main components in Xiaoyao Pills is mainly focused on paeoniflorin, the main active component, and the detection index is single. Complete the content determination under the one-test and multiple-evaluation system for multiple components
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Embodiment 1
[0040] Instruments used in the following examples
[0041] laboratory apparatus
[0042]Shimadzu IC-20AT high performance liquid chromatograph (SPD-M20A diode array detector), BP211D type 1 / 10,000 precise analytical balance (Sartorius), FA1004N 1 / 100,000 precise electronic analytical balance (Sartorius) Sri Lanka), HH-6 digital display constant temperature water bath (Changzhou Jiangnan Experimental Instrument Factory), SHZ-D (III) circulating water vacuum pump (Gongyi Yuhua Instrument Co., Ltd.), pipette gun (100- 1000μL, Dalong Xingchuang Instruments), KQ5200DE CNC ultrasonic cleaner (frequency: 40KHz, Kunshan Ultrasonic Instrument Co., Ltd.). CAMAG thin-layer chromatography scanner (LINOMAT5), CAMAG TLCVisualizer thin-layer chromatography imaging and file system, traditional Chinese medicine chromatographic fingerprint similarity evaluation system version A.
[0043] 1.2 Experimental reagents and materials
[0044] Poria reference medicinal material (batch number 121117-...
Embodiment 2
[0066] The methodological research of embodiment 2 fingerprint spectrum detection:
[0067] 1. Methodological investigation
[0068] 1.1 Precision experiment
[0069] Prepare a part of need testing solution according to Example 1, according to the chromatographic conditions described in Example 1, continuous sampling 6 times, record the retention time and peak area of 9 groups of contrast peaks, calculate and obtain each component peak area and retention The RSDs of time are all less than 3%, and the results show that the precision of this method is good.
[0070] 1.2 Repeatability experiment
[0071] Prepare a part of need testing solution according to embodiment 1, according to the chromatographic condition described in embodiment 1 sample injection detection, record the peak area and the retention time of 9 groups of contrast peaks, calculate the peak area and retention time that can get each composition The RSDs were all less than 3%, and the results showed that the m...
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Abstract
The invention discloses a fingerprint spectrum detection method of Xiaoyao pills. The method comprises the following steps: step 1, preparing a Xiaoyao pill test solution; 2, preparing a mixed reference substance solution; 3, respectively and precisely sucking the test sample and the reference substance solution, injecting the test sample and the reference substance solution into a liquid chromatogram instrument, and recording chromatograms; and 4, exporting the Xiaoyao pill fingerprint spectrum instrument, importing into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, selecting chromatographic peaks existing in the chromatograms of different batches of Xiaoyao pills as common peaks, generating a contrast fingerprint spectrum of the Xiaoyao pills by using an average value calculation method, and calculating the relative retention time and the relative peak area of each common peak. The Xiaoyao pill fingerprint spectrum researched by the invention can comprehensively and objectively evaluate the quality of Xiaoyao pills. The detection method provided by the invention has the advantages of good stability, high accuracy, good repeatability, high precision and the like.
Description
technical field [0001] The invention belongs to the technical field of Chinese patent medicine detection, and in particular relates to a fingerprint detection method of Xiaoyao Pills. Background technique [0002] Xiaoyao Pills originated from the book of Chinese medical prescriptions in the Song Dynasty - "Taiping Huimin Heji Bureau Prescription". It is clinically used to treat depression, chest pain, dizziness, loss of appetite and irregular menstruation caused by liver depression and spleen deficiency. "Chinese Pharmacopoeia" 2015 Edition Zhongxiaoyao Pills Take Paeoniflorin Content as Index to Control the Quality [1] In the standard issued by the national ministry, the identification reaction of Angelica and Radix Paeoniae Alba [2] for quality control standards. [0003] Xiaoyao Pills are widely used in TCM clinical practice, so relevant scholars also completed quality control and evaluation by testing some active ingredients of Xiaoyao Pills. Among them, Xu Yu et al....
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