Method for preparing oligosaccharide from pseudo-ginseng

Abstract

The invention discloses a method for preparing oligosaccharide from pseudo-ginseng, which comprises the following steps: (1) drying pseudo-ginseng, crushing or slicing, extracting with ethanol, filtering and concentrating to obtain extract; (2) diluting the extract with water, adsorbing and eluting through non-polar macroporous resin, collecting upper column effluent and eluent, combining and concentrating to obtain concentrated water; (3) adding ethanol into the concentrated water solution, refrigerating and standing, and passing through an organic ultrafiltration membrane after precipitate appears, so as to obtain an organic ultrafiltration solution; (4) enabling the organic ultrafiltrate to pass through an ion exchange column, collecting effluent, and recovering ethanol to obtain an oligosaccharide aqueous solution; (5) enabling the oligosaccharide aqueous solution to pass through cation exchange resin, and collecting effluent; and (6) decolorizing the effluent, and freeze-drying to obtain the oligosaccharide. Compared with the prior art, the method has the advantages that the cost is low, the operation is simple, the yield and the purity are higher, and the prepared oligosaccharide does not contain substances such as galacturonic acid, polygalacturonic acid methyl ester, tannin, tannin and the like, and is more suitable for patients suffering from hyperacidity to take.

Description

technical field [0001] The invention relates to the technical field of extraction of plant active ingredients, in particular to a method for preparing oligosaccharides from Panax notoginseng. Background technique [0002] Oligosaccharides, also known as oligosaccharides, are a general term for low-polymerized sugars with straight or branched chains that contain 2-10 monosaccharides that are dehydrated and condensed by glycosidic bonds. The sugars in between are called oligosaccharides, which can be divided into functional oligosaccharides and ordinary oligosaccharides. Functional oligosaccharides are widely distributed in nature and there are many kinds. Because of their characteristics of low calorie, intestinal probiotics, anti-constipation, lowering serum cholesterol, enhancing immunity, and anti-tumor, they have become important basic raw materials in the production of functional foods And food additives, has been used in industrial production in many countries in the w...

AI Technical Summary

Problems solved by technology

The mixed product of notoginseng oligosaccharides and notoginseng amino acids has a good improvement effect on patients with anemia, hypotension, insomnia, and immunocompromised patients. Oral gavage of the mixed product to mice with gastric loss, or to patients with old stomach disease, the daily oral administration of 10ml will aggravate the patient's stomach pain. Tannin, tanning and other substances, these substances are acidic, sour and heavy, overpowering the sweetness, which can improve intestinal metabolism for normal people, but taking it for patients with hyperacidity may aggravate stomach pain

However, after the conventional water extraction method, the ethanol fractionation precipitation process will more or less coat the notoginseng oligosaccharides and precipitate them. The higher the concentration of ethanol alcohol precipitation, the higher the concentration of oligosaccharides, and the greater the loss of oligosaccharides when precipitating polysaccharides , resulting in a lower yield

Images