Preparation method and application of fluorescence-colorimetric dual-mode sensor for detecting Hg < 2 + > in water
A dual-mode, sensor technology, applied in fluorescence/phosphorescence, instruments, measurement devices, etc., can solve the problems of high background signal, limited application of sensing equipment, limited colorimetric inspiration, etc., to simplify the experimental process and avoid data. Unreliable, highly accurate effects
Active Publication Date: 2022-03-18
JIANGSU UNIV
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Problems solved by technology
While the latter avoids the problem of non-specific aggregation of nanoparticles, the sensitivity of colorimetric methods is relatively limited or poor
The fluorescence method can achieve sensitive detection by changing the color and intensity of the fluorescent material, but the background signal is high
Each technology has its shortcomings, which greatly limit the application of these sensing devices
Method used
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Embodiment 1
[0040] (1) Synthesis of P-CeO 2 NR;
[0041] In the first step of hydrothermal synthesis, 25 mL of 0.4M Ce(NO 3 ) 3 ·6H 2 The O solution was mixed with 175 mL of 6.8M NaOH solution and stirred vigorously at room temperature for 30 min. Then, in a high borosilicate heat-resistant glass bottle, the mixture was hydrothermally treated at 100 °C for 17 h; the product was centrifuged and washed three times with ethanol and water, and dried at 60 °C for 12 h to obtain a non-porous nanorod precursor. In the second step of hydrothermal synthesis, 20 mg of the nonporous nanorod precursor was dispersed in 10 mL of ultrapure water, and hydrothermally treated at 160 °C for 12 h in a polytetrafluoroethylene-lined autoclave. The pale yellow product was collected by centrifugation at 8000rpm for 10min, washed with ethanol and water, centrifuged, and dried at 60°C for 12h to obtain P-CeO 2 Nr.
[0042] (2) Mixed P-CeO 2 NR solution and FAM-apt solution;
[0043] The FAM-apt purchase...
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Abstract
The invention belongs to the technical field of nano sensing detection, and relates to a preparation method and application of a fluorescence-colorimetric dual-mode sensor for detecting Hg < 2 + > in water. Colorless TMB (tetramethylbenzidine) is oxidized into a blue oxidation-state product to generate a colorimetric signal by utilizing the peroxidase-like catalytic characteristic of P-CeO2NR, a fluorescence signal is generated by utilizing FAM-apt, and the fluorescence-colorimetric dual-mode sensor is obtained through the fluorescence quenching effect between the P-CeO2NR and the FAM-apt, the peroxidase-like activity enhancing characteristic and the characteristic that an aptamer is specifically combined with Hg < 2 + >; the fluorescence linear response range of the dual-mode sensor constructed by the invention to Hg < 2 + > is 0.08-12.5 nM, and the detection limit is 0.079 nM; the colorimetric linear response range is 0.5-100 nM, the detection limit is 0.31 nM, accurate detection of Hg < 2 + > is achieved, and an effective way is provided for visual detection.
Description
technical field [0001] The invention belongs to the technical field of nano-sensing detection, and relates to a method for preparing a fluorescence-colorimetric dual-mode sensor based on porous ceria nanorods, which is used to detect Hg 2+ Accurate and visual detection. Background technique [0002] Mercury ion (Hg 2+ ) is the most stable form of mercury in our daily life and is considered to be one of the most toxic heavy metal ion pollutants. Hg 2+ It can directly or indirectly enter animals and humans through food chains, etc., and can produce bioaccumulative effects in humans. Even at low concentrations, Hg 2+ It will also pose a huge threat to human health, such as destroying human body functions, causing skin ulcers, brain damage and other irreversible consequences. Therefore, the detection of Hg in the environment 2+ is very necessary. [0003] Currently, colorimetric and fluorometric methods are widely used to detect Hg 2+ . Colorimetry is attractive because ...
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IPC IPC(8): G01N21/64G01N21/31G01N21/78
CPCG01N21/643G01N21/31G01N21/78G01N2021/6432Y02A20/20
Inventor 李丽波李佳敏由天艳毕晓雅
Owner JIANGSU UNIV