Application of magnolol in relieving cytotoxicity induced by fumonisins B1
A fumonisin and cell-inducing technology, applied in the field of biomedicine, can solve problems affecting cell signal transduction, cell membrane structure damage, redox state imbalance, etc., and achieve broad application prospects and the effect of cytotoxicity mitigation
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Embodiment 1
[0025] Example 1: Determination of Cell Viability
[0026] After the rat C6 glioma cells (C6 cells) in the logarithmic growth phase were passaged, the cell density was adjusted to 7×10 4 cells / mL were inoculated into 96-well plates, and after the cells grew for 24 hours, different exposure treatments were performed:
[0027] Blank control group (Control);
[0028] FB1 exposure group: 15μM FB1;
[0029] 40μM MAG treatment group: 15μM FB1+40μM MAG;
[0030] 80μM MAG treatment group: 15μM FB1+80μM MAG;
[0031] 160μM MAG treatment group: 15μM FB1+160μM MAG;
[0032] After 24 hours of treatment, 20 μL of 5 mg / mL MTT solution was added to each well under dark conditions, and placed in a cell culture incubator (conditions were 37 ° C, 5% CO 2 ) for a further 4 h. Add dimethyl sulfoxide solution, measure its absorbance value at 570nm and obtain the cell viability of each group. Taking the absorbance of cells in the blank control group as 100%, the cell viability of each group ...
Embodiment 2
[0034] Example 2: The effect of FB1 exposure on cell morphology and the repairing effect of different concentrations of MAG
[0035] The C6 cells were treated according to the exposure treatment method in Example 1; the morphology of the C6 cells in different groups was observed with a scanning electron microscope.
[0036] Such as figure 2 As shown, under the scanning electron microscope, the morphology of the cells in the control group was normal, the cells were spindle-shaped, the cell membrane was smooth, and the boundaries were clear. After being exposed to 15μM FB1 for 24 hours, the cell synapses retracted or even disappeared, the cell volume became smaller, the surface of the cell membrane was rough, and there were irregular protrusions, showing the morphology of apoptosis. After treatment with different concentrations of MAG, cell synapses re-extended and cell membrane surfaces tended to be smooth. Among them, the volume and morphology of cells treated with 80μMMAG ...
Embodiment 3
[0038] Example 3: The effect of FB1 exposure on apoptosis and the repairing effect of different concentrations of MAG
[0039] The C6 cells were treated according to the exposure treatment method in Example 1; the cells were stained with DAPI, and the morphological changes of the nuclei were observed under a fluorescence microscope, so as to judge the cell apoptosis.
[0040] Such as image 3 As shown, in the control group, the nuclei were round, the chromatin was evenly distributed, and no obvious morphological changes related to apoptosis were seen. In the FB1 exposure group, the cell nuclei were closely arranged, accompanied by typical apoptotic pathological changes, and accompanied by the appearance of apoptotic bodies. After treatment with different concentrations of MAG, the number of nucleus pyknosis and apoptotic bodies decreased significantly, and most of the chromatin showed a uniform blue color.
[0041] DAPI staining results showed that MAG could inhibit the apop...
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