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Method for preparing controllable high-concentration silk fibroin solution by using tangential flow ultrafiltration technology

A technology of tangential flow ultrafiltration and silk fibroin, which is applied in the field of preparation of controllable high-concentration silk fibroin solution by tangential flow ultrafiltration technology, can solve the problems of complicated steps, poor mechanical properties, high cost, etc., and achieve improved Effects of transparency, efficiency improvement, and performance improvement

Pending Publication Date: 2022-03-22
FAVORSUN MEDICAL TECH (SUZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the actual work of these years, this patented method has many procedures or complicated steps, and its production raw materials cannot be reused or the cost of recycling is high
At the same time, some researches have invented a low-temperature freeze-drying method to prepare silk fibroin powder with a molecular weight lower than 80kDa. However, due to the small molecular weight of the powder, its mechanical properties are relatively poor, and it cannot bear the characteristics of biological materials as high-strength materials.

Method used

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  • Method for preparing controllable high-concentration silk fibroin solution by using tangential flow ultrafiltration technology
  • Method for preparing controllable high-concentration silk fibroin solution by using tangential flow ultrafiltration technology
  • Method for preparing controllable high-concentration silk fibroin solution by using tangential flow ultrafiltration technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] (1) Preparation of silk fibroin salt solution: dissolving silk fibroin after degumming, washing and drying in 9.3M lithium bromide (LiBr) solution to form a salt solution with a concentration of 10g / 100ml.

[0105] (2) Clarification experiment:

[0106] a) The initial turbidity of the test sample stock solution is 244NTU.

[0107] b) Assemble the filter device according to the schematic diagram, and select a filter with a polypropylene membrane material of 0.45um, such as image 3 shown.

[0108] c) Rinse the soaking test pipeline with 1M NaOH solution, and then rinse it with pure water.

[0109] d) Put the sampling tube into the sample bottle, adjust the pump speed to about 20-30mL / min, start filtering, and record the filtering time, filtrate volume, pressure gauge reading, etc. in real time, such as Figure 5 shown.

[0110] e) When the feed liquid in the sample bottle is filtered, stop the filtration test.

[0111] f) Collect the filtrate, and detect that the tu...

Embodiment 2

[0122] (1) Preparation of silk fibroin salt solution: dissolving silk fibroin after degumming, washing and drying in 9.3M lithium bromide (LiBr) solution to form a salt solution with a concentration of 10g / 100ml.

[0123] (2) Clarification experiment:

[0124] a) Assemble the filter device according to the schematic diagram, and select a filter with a polypropylene membrane material of 0.45um, such as image 3 shown.

[0125] b) Rinse the pipeline for immersion test with 1M NaOH solution, and then rinse it with pure water.

[0126] c) Put the sampling tube into the sample bottle, adjust the pump speed to about 20-30mL / min, and start filtering.

[0127] d) When the feed liquid in the sample bottle is filtered, stop the filtration test.

[0128] e) Collect the filtrate.

[0129] (3) Desalting the silk fibroin salt solution:

[0130] a) Install a tangential flow filtration system such as Figure 4 shown.

[0131] b) Rinse the system pipeline with 1M NaOH solution and pure ...

Embodiment 3

[0139] (1) Preparation of silk fibroin salt solution: the silk fibroin after degumming, washing and drying of silk was dissolved in 9.3M lithium bromide (LiBr) solution to form a salt solution with a concentration of 5g / 100ml.

[0140] (2) Clarification experiment:

[0141] a) Assemble the filter device according to the schematic diagram, and select a filter with a polypropylene membrane material of 0.45um, such as image 3 shown.

[0142] b) Rinse the pipeline for immersion test with 1M NaOH solution, and then rinse it with pure water.

[0143] c) Put the sampling tube into the sample bottle, adjust the pump speed to about 20-30mL / min, and start filtering.

[0144] d) When the feed liquid in the sample bottle is filtered, stop the filtration test.

[0145] e) Collect the filtrate.

[0146] (3) Desalting the silk fibroin salt solution:

[0147] a) Install a tangential flow filtration system such as Figure 4 shown.

[0148] b) Rinse the system pipeline with 1M NaOH solu...

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Abstract

The invention relates to a method for preparing a controllable high-concentration silk fibroin solution by utilizing a tangential flow ultrafiltration technology. The method comprises the following steps: obtaining an inorganic salt solution of silk fibroin; carrying out membrane clarification treatment on the inorganic salt solution of the silk fibroin; desalting treatment of the inorganic salt solution of the silk fibroin: filtering the clear liquid subjected to membrane clarification treatment through a tangential flow filtering system; and concentrating the silk fibroin solution to obtain a high-concentration high-molecular-weight silk fibroin aqueous solution. Compared with the prior art, desalination and concentration of the silk fibroin salt solution are achieved through the tangential flow technology, and therefore the silk fibroin aqueous solution with the number-average molecular weight ranging from 80 kDa to 200 kDa is directly obtained. The performance of the obtained material in all aspects is improved. Meanwhile, the efficiency is greatly improved, the cost is saved, and more convenience is provided for development of silk fibroin materials.

Description

technical field [0001] The invention belongs to the technical field of silk protein treatment, and in particular relates to a method for preparing a controllable high-concentration silk fibroin solution by using tangential flow ultrafiltration technology. Background technique [0002] Silk is one of the natural fibers with the best strength. Silk is composed of 70-80% silk fibroin and 20-30% sericin. In addition to being used as traditional textile materials, silk protein can also be used as advanced biopolymer materials. Since sericin can cause inflammation in the body, silk must be degummed before being used as a biological material. A large number of studies have shown that silk fibroin obtained after silk degumming and other steps has good biocompatibility, and its inflammatory response in vivo is much lower than that of commonly used biological materials such as collagen and polylactic acid. As a natural material with excellent physical and chemical properties, silk f...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/34
CPCC07K14/43586B01D2315/10B01D61/145B01D2315/16B01D61/16B01D2311/04B01D2311/2649
Inventor 刘也卓杨文华
Owner FAVORSUN MEDICAL TECH (SUZHOU) CO LTD