IBD egg yolk antibody microsphere and preparation method thereof
A technology of egg yolk antibody and microspheres, which is applied in the direction of antibodies, antibody medical components, anti-inflammatory agents, etc., can solve the problems of easy stress response and large injection volume, so as to improve drug stability, reduce toxic side effects, and enhance immunity effect of effect
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Embodiment 1
[0029] A preparation method for IBD egg yolk antibody microspheres, comprising the following steps:
[0030] 1) Mix 5g Polygonatum polysaccharide and 5ml IBD egg yolk antibody and stir evenly;
[0031] 2) Add the solution material obtained in step 1) into 10ml Bupleurum essential oil, homogenize and emulsify with a homogenizer, and use it as the inner phase of the microspheres;
[0032] 3) Dissolve 1g of polylactic-co-glycolic acid (PLGA) in 75ml of dichloromethane to obtain a solution as the external phase of microspheres;
[0033] 4) Mix the solution of step 2) with the solution of step 3) to obtain a mixed solution (the volume ratio of internal and external phases is 1:5), add 9ml of surfactant PEG400 to the mixed solution, and perform high-shear emulsification at a rate of 10000 r / min, increasing The affinity between the inner phase and the outer phase makes the active ingredients of the inner phase encapsulated in the outer phase of PLGA;
[0034] 5) The mixture in step...
Embodiment 2
[0036] A preparation method for IBD egg yolk antibody microspheres, comprising the following steps:
[0037] 1) Mix 7g Polygonatum polysaccharide and 5ml IBD egg yolk antibody and stir evenly;
[0038] 2) Add the solution material obtained in step 1) into 10ml Bupleurum essential oil, homogenize and emulsify with a homogenizer, and use it as the inner phase of the microspheres;
[0039] 3) Dissolve 1g of polylactic-co-glycolic acid (PLGA) in 60ml of dichloromethane to obtain a solution as the external phase of microspheres;
[0040] 4) Mix the solution of step 2) with the solution of step 3) to obtain a mixed solution (volume ratio of internal and external phases: 1:4), add 7ml of surfactant PEG400 to the mixed solution for homogenization, and perform high-shear emulsification at a rate of 10000 r / min , increase the affinity between the inner phase and the outer phase, so that the active ingredients of the inner phase are encapsulated in the outer phase of PLGA;
[0041] 5) ...
Embodiment 3
[0043] A preparation method for IBD egg yolk antibody microspheres, comprising the following steps:
[0044] 1) Mix 3g Polygonatum polysaccharide and 5ml IBD egg yolk antibody and stir evenly;
[0045] 2) Add the solution material obtained in step 1) into 10ml Bupleurum essential oil, homogenize and emulsify with a homogenizer, and use it as the inner phase of the microspheres;
[0046] 3) Dissolve 1g of polylactic-co-glycolic acid (PLGA) in 120ml of dichloromethane to obtain a solution as the external phase of microspheres;
[0047] 4) Mix the solution of step 2) with the solution of step 3) to obtain a mixed solution (volume ratio of internal and external phases: 1:8), add 13ml of surfactant PEG400 to the mixed solution for homogenization, and perform high-shear emulsification at a rate of 10000 r / min , increase the affinity between the inner phase and the outer phase, so that the active ingredients of the inner phase are encapsulated in the outer phase of PLGA;
[0048] 5...
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