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Method for extracting diatom shells

A technology of diatom shells and algae liquid, applied in the directions of silicon oxide and silicon dioxide, can solve the problems of the danger of concentrated sulfuric acid, adhesion of diatom shells, poor safety, etc., and achieve high safety, clear pores, and low pollution. Effect

Pending Publication Date: 2022-03-25
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of this patent are: the purity of the diatom shells is not high, a large amount of impurity ions remain, and the diatom shells adhere to each other
The disadvantage of this patent is that the use of concentrated sulfuric acid is dangerous, and for some diatoms, hydrogen peroxide is not enough to remove all organic matter
The main disadvantages of this patent are: consumption of a large amount of concentrated acid, poor safety, easy to cause pollution

Method used

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  • Method for extracting diatom shells
  • Method for extracting diatom shells
  • Method for extracting diatom shells

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Example 1: Navicula sp. is selected, treated with hydrochloric acid, hydrogen peroxide, and absolute ethanol, and calcined in an air atmosphere to obtain diatom shells

[0045] (1) Pour out the supernatant in the culture container, scrape the Navicula attached to the container wall, and obtain the Navicula enriched algae liquid;

[0046] (2) adding hydrochloric acid with a concentration of 1M to the navicula enriched algae liquid in (1), ultrasonically cleaning for 2 hours to fully react hydrochloric acid and carbonate to obtain a hydrochloric acid mixture;

[0047] Dosage: Add 400mL dilute hydrochloric acid to 100mL enriched algae liquid

[0048] (3) centrifuging the hydrochloric acid mixture in (2) at a rotating speed of 4000rpm for 10min, removing the supernatant, and obtaining the Navicula precipitate after hydrochloric acid treatment;

[0049](4) Add 10% hydrogen peroxide to the Navicula precipitate after hydrochloric acid treatment in (3), ultrasonicate in a wate...

Embodiment 2

[0060] Embodiment 2: Select Navicula sp., use hydrochloric acid, hydrogen peroxide, absolute ethanol to process, and calcine under nitrogen atmosphere to obtain Navicula shell

[0061] (1) Pour out the supernatant in the culture container, scrape the Navicula attached to the container wall, and obtain the Navicula enriched algae liquid;

[0062] (2) adding hydrochloric acid with a concentration of 1M to the navicula enriched algae liquid in (1), ultrasonically cleaning for 3 hours to fully react hydrochloric acid and carbonate to obtain a hydrochloric acid mixture;

[0063] Dosage: Add 400mL dilute hydrochloric acid to 100mL enriched algae liquid

[0064] (3) centrifuging the hydrochloric acid mixture in (2) at a rotating speed of 4000rpm for 10min, removing the supernatant, and obtaining the Navicula precipitate after hydrochloric acid treatment;

[0065] (4) Add 30% hydrogen peroxide to the Navicula precipitate after hydrochloric acid treatment in (3), ultrasonicate in a wa...

Embodiment 3

[0074] Example 3: Cyclotella sp. is selected, treated with nitric acid, hydrogen peroxide, and acetone, and calcined in an oxygen atmosphere to obtain diatom shells

[0075] (1) Filtrating the Cyclotella in the culture container to obtain a Chlorella-enriched algae liquid;

[0076] (2) Adding nitric acid with a concentration of 2M to (1) Cyclotella mesocellae-enriched algae liquid, ultrasonic cleaning for 1 hour to fully react nitric acid and carbonate to obtain a nitric acid mixture;

[0077] Dosage: Add 300mL dilute nitric acid to 100mL enriched algae liquid

[0078] (3) Centrifuge the nitric acid mixture in (2) at a rotating speed of 5000rpm for 5min, remove the supernatant, and obtain the Cyclotella precipitate after nitric acid treatment;

[0079] (4) Adding concentration of 20% hydrogen peroxide to the Cyclotella precipitate treated with hydrochloric acid in (3), 50 DEG C of water bath ultrasonic 2h, carries out oxidative digestion, destroys the Cyclotella cell membrane...

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Abstract

The invention discloses a method for extracting diatom shells, which comprises the following steps of: removing carbonate in diatom by using dilute acid, carrying out simple oxidative digestion on the diatom by using hydrogen peroxide, destroying cell membranes, removing pigments and impurity ions by using ethanol / methanol / acetone, and removing organic matters attached to the diatom shells by using calcination. The high-purity extraction of the diatom shells is completed. According to the present invention, the method has characteristics of high safety and no environmental pollution, the morphology and the microstructure of the original species diatom of the diatom shell can be retained, the energy disperse spectroscopy test result shows that the impurity ions and the organic matter can be effectively removed, and the obtained diatom shell has the high purity.

Description

technical field [0001] The invention belongs to the technical field of extracting diatom shells, in particular to a method for obtaining complete and pure diatom shells from marine diatoms. Background technique [0002] Diatoms, unicellular eukaryotic algae, have been found to have more than 100,000 species, with various forms, and have a manufacturing component of amorphous hydrated silicon dioxide (SiO 2 ·nH 2 O) The function of the shell. Under the action of biosiliconization, diatom shells form a repeatable fine, complex, regular and symmetrical hierarchical porous structure at the micro / nano level. [0003] Diatom shell is a natural three-dimensional biological material with large specific surface area, high adsorption, high compressive strength, chemical stability and good biocompatibility, and has been widely used in chemical and environmental fields. , in recent years, it has also received some research and development in the fields of drug delivery and energy sto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B33/18
CPCC01B33/18C01P2006/80
Inventor 刘红昌陈宇新夏金兰聂珍媛申泽
Owner CENT SOUTH UNIV