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Patch clamp record analysis method and system

A technology of record analysis and patch clamp, applied in the field of patch clamp, can solve the problems of waste of time and manpower, relying on experience, etc., achieve high precision and accuracy, avoid experience accumulation, and reduce time and labor costs

Pending Publication Date: 2022-04-05
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Based on this, it is necessary to provide a patch clamp recording analysis method and system for the problems of relying heavily on experience and wasting time and manpower in the manual operation of the above-mentioned existing patch clamp technology

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  • Patch clamp record analysis method and system
  • Patch clamp record analysis method and system

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Embodiment Construction

[0047] In order to make the purpose, technical solutions and advantages of the present application more clearly understood, the present application will be described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application, but not to limit the present application.

[0048] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which this application belongs. The terms used herein in the specification of the application are for the purpose of describing specific embodiments only, and are not intended to limit the application.

[0049] It will be understood that the terms "first", "second", etc. used in this application may be used herein to describe various elements, but these elements are not limited by these terms. T...

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Abstract

The invention relates to a patch clamp recording and analyzing method and system. The patch clamp recording and analyzing method comprises the following steps: acquiring a tip target position of a recording electrode and acquiring a cell target position of a detected cell; wherein the cell target position and the tip target position are obtained according to view image data of the microscope and a preset target classification model; performing conversion according to the cell target position and the tip target position to obtain plane movement displacement; outputting a movement instruction to the micro-operation device according to the plane movement displacement to control the recording electrode to move to be in contact with the detected cell; and outputting a negative pressure control instruction to the pressure device, so that the pressure device provides preset negative pressure until the recording electrode finishes sealing and / or membrane rupture with the detected cell. According to the method, the visual field image data of the microscope is collected for image recognition to obtain the positions of the recording electrode and the detected cell, and the recording electrode is controlled to complete contact, sealing and membrane rupture with the detected cell, so that not only is the precision and accuracy higher, but also the process is prevented from depending on experience accumulation, and the time cost and the labor cost are also reduced.

Description

technical field [0001] The present application relates to the field of patch clamp technology, and in particular, to a method and system for patch clamp recording and analysis. Background technique [0002] The patch clamp technique is to use glass electrodes to seal the cell membrane with only 1-3 ion channels on the cell with an area of ​​several square microns through negative pressure suction for high resistance sealing. Microelectronics technology that records electrophysiological properties, especially single-channel currents. It is widely used to study the electrical properties of cells and the effects of small molecule drugs on the properties of ion channels on the cell membrane. At present, with the development and progress of technology, it can also be used for ion pumps, exchangers, and the endocytosis and exocytosis mechanisms of excitable cells. research etc. [0003] In the patch clamp technique, it is necessary to find the glass electrode and the measured ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/483G01N15/10
Inventor 校世杰陈放怡李昌日
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA