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Application of a kind of human platelet apoptosis microvesicle

A technology of microvesicles and platelets, which is applied to blood/immune system cells, bone/connective tissue cells, prostheses, etc., can solve problems such as unsatisfactory effects, avoid ethical problems and immune problems, and the preparation method is simple and broad. The effect of clinical application prospects

Active Publication Date: 2022-05-31
PEKING UNIV SCHOOL OF STOMATOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the problems and unsatisfactory effects of existing bone defect repair treatment methods, the present invention provides a safe and efficient apoptotic microvesicle that promotes bone regeneration, aiming to solve the existing bone defect repair treatment effects and promote its therapeutic application

Method used

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  • Application of a kind of human platelet apoptosis microvesicle
  • Application of a kind of human platelet apoptosis microvesicle
  • Application of a kind of human platelet apoptosis microvesicle

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Embodiment 2

[0072] (2) Analyze by NTA analysis software.

Embodiment 3

[0080] After 10 days of osteogenic induction, the effect of the cells on osteogenic differentiation was examined by alizarin red staining.

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Abstract

The invention discloses the application of human platelet apoptosis microvesicles in the preparation of a preparation for promoting the osteogenic differentiation of mesenchymal stem cells, and provides a bone defect repair preparation, which includes a PLGA scaffold material, and the surface of the PLGA scaffold material carries the aforementioned Human platelet apoptotic microvesicles. The apoptotic microvesicles derived from human platelets provided by the invention can be extracted from the patient's own blood, avoiding ethical and immune problems. The bone defect repair preparation provided by the present invention, which is constructed by carrying human platelet apoptotic microvesicles on the PLGA / pDA scaffold, improves the shortcomings of PLGA's hydrophobicity and low osteogenic activity, especially after loading a layer of pDA film on the surface of the PLGA scaffold Furthermore, it is equipped with human platelet apoptosis microvesicles, so that the human platelet apoptosis microvesicles can be slowly released in vivo, further improving the osteogenesis ability of mesenchymal stem cells in vivo.

Description

Application of a kind of human platelet apoptotic microvesicles technical field [0001] The present invention relates to the technical field of biological tissue engineering, in particular to the application of a human platelet apoptosis microvesicle. Background technique [0002] Apoptosis is a highly regulated cell death process in which specific cells are sacrificed for a larger organism Benefit. This is a normal physiological process in multicellular organisms. Apoptosis confers superiority to multicellular organisms in a coordinated manner potential to maintain steady state and fine-tune the life cycle. Death by apoptosis goes through several stages, starting with nuclear chromatin condensation, followed by membrane vesicle rupture, followed by disintegration of cellular contents into distinct membrane-encapsulated vesicles called apoptotic bodies or apoptotic bodies Apoptotic vesicles (apoVs). Apoptotic microvesicles differ from exosomes (also called exosomal ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775A61K35/19A61P19/08A61L27/34A61L27/18A61L27/54C12N5/078
CPCA61K35/19A61L27/18A61L27/34A61L27/54A61P19/08C12N5/06
Inventor 周永胜张晓江雨荷邵玉子杨坤坤
Owner PEKING UNIV SCHOOL OF STOMATOLOGY