Chitosan and silk peptide protein compounded biomimetic material as well as preparation method and application thereof

A technology of silk peptide protein and bionic materials, applied in medical science, prosthesis, etc., can solve the problems of poor mechanical properties and water resistance, achieve good antibacterial properties, excellent mechanical properties, and promote the effect of β-folding

Pending Publication Date: 2022-04-12
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Natural biopolymers such as polysaccharides (such as chitosan, cellulose, starch, and alginate) and pr

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0028] The present invention provides a method of preparing a bionic material of chitosan and thorns protein composite, including the following steps:

[0029] The chitosan, formic acid and thoricin protein were mixed with polymer solution to obtain a complex product;

[0030] The complex product is extruded to obtain a membrane material;

[0031] The membrane material was sequentially recrystallized and acid-base, and the bionic material of the chitosan and thorns protein composite was obtained.

[0032] In the present invention, if there is no special instruction, the raw materials used are commercially available goods in the art.

[0033] The invention is mixed with chitosan, formic acid and thoricin protein to obtain a complexation product. In the present invention, the caric acid can proton chitosan, so that the chitosan protonated tape is positively charged, and the positively charged chitosan and a negatively charged thorns, and the formic acid is increased. The action of t...

Example Embodiment

[0062] Example 1

[0063] 25 wt.% Of 150,000 molecules were mixed with 50 wt.% Formic acid to obtain protonated chitosan cucumic substances, then mixed with 25 wt.% Thorns protein, sealing 2 days. The resulting mixture was placed in an extruder. The extruder is divided by the six-stage temperature of the screw started in the addition of the extruded opening in order 40 ° C, 60 ° C, 95 ° C, 80 ° C, 90 ° C and 90 ° C, the die temperature of 90 ° C. The head pressure is 20 MPa. After the extrusion is completed, the film is removed, cooled to room temperature. The film was soaked in a methanol aqueous solution of 2 days, followed by another day in 4% (w / v) sodium hydroxide solution (pH = 13.60 ± 0.04), then removed the residual formic acid, and then thoroughly washed with distilled water to remove residual Chemical material. Observation shows that if there is no sodium hydroxide solution treatment, materials with high integrity and strong performance cannot be obtained. Finally, the...

Example Embodiment

[0065] Example 2

[0066] 10 wt.% Of 200,000 molecules were mixed with 30 wt.% Formic acid to give a protonated chitosan cucumic substance, then mixed with 60 wt.% Thorns protein, sealing 2 days. The resulting mixture was placed in an extruder. The extruder is divided by the six-stage temperature of the screw started in the addition of the extruded opening in order 40 ° C, 60 ° C, 95 ° C, 80 ° C, 90 ° C and 90 ° C, the die temperature of 90 ° C. The head pressure is 26 MPa. After the extrusion is completed, the film is removed, cooled to room temperature. The film was soaked in a methanol aqueous solution of 2 days, followed by another day in 4% (w / v) sodium hydroxide solution (pH = 13.60 ± 0.04), then removed the residual formic acid, and then thoroughly washed with distilled water to remove residual Chemical material. Finally, the sample was dried in an oven at a 40 ° C oven for 1 day, and when dried. The sample of the dry end was placed in a temperature of 25 ° C, and the hum...

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Abstract

The invention provides a chitosan and silk peptide protein compounded biomimetic material as well as a preparation method and application thereof, and belongs to the field of natural high polymer materials. Chitosan, formic acid and silk peptide protein are mixed for polyelectrolyte complexation, and a complexation product is obtained; carrying out extrusion molding on the complexing product to obtain a film-shaped material; and sequentially carrying out recrystallization and acid-base neutralization on the membranous material to obtain the chitosan and silk peptide protein compounded biomimetic material. According to the method, silk peptide protein and chitosan are plasticized through formic acid, the formic acid can enable the chitosan to be protonized and positively charged so as to be combined with the silk peptide protein with negative electricity, polyelectrolyte complexation (PEC) is utilized, the silk peptide protein is negatively charged, and the silk peptide protein and the positively charged chitosan are subjected to anion and cation exchange reaction, so that the silk peptide protein can be combined with the silk peptide protein with negative electricity. The micromolecular silk peptide protein can be firmly fixed on the chitosan through electrostatic interaction, so that the composite material with excellent mechanical property and good water resistance is obtained, and the conversion control of rigidity and elasticity of the composite material can be realized by controlling the moisture content.

Description

technical field [0001] The invention relates to the technical field of natural polymer materials, in particular to a biomimetic material compounded with chitosan and silk peptide protein and its preparation method and application. Background technique [0002] In recent years, there has been a great deal of interest in developing materials from natural biopolymers. Natural biopolymers have shown great potential in the fields of tissue engineering, environment, biomedicine, electronic materials, etc. Because of the unique properties of natural biopolymers (low cost, universality, renewability, non-toxicity, biocompatibility, and biodegradability, etc.), they are more advantageous than traditional synthetic polymers. Traditional synthetic polymers such as polyethylene, polypropylene and other petroleum-based compounds are almost ubiquitous in daily life, but synthetic polymers face many challenges, including environmental issues, cross-contamination, and toxicity risks to con...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/22A61L27/50
Inventor 袁小凡胡董霞张林华孟令晗高成成汤晓智
Owner NANJING UNIV OF FINANCE & ECONOMICS
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