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Method for detecting contents of retinol and six retinol derivatives in cosmetics

A detection method and technology for retinol, which can be applied to measurement devices, color/spectral property measurement, instruments, etc., can solve the problems of lack of retinol and 6 kinds of retinol derivatives content determination methods, and achieve reliable sample separation. , Improve detection efficiency and high sensitivity

Active Publication Date: 2022-04-12
星智豫美(上海)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a detection method for the content of retinol and 6 retinol derivatives in cosmetics, which is used to solve the lack of detection of retinol in cosmetics and raw materials in the prior art. And the problem of the content determination method of 6 kinds of retinol derivatives

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  • Method for detecting contents of retinol and six retinol derivatives in cosmetics
  • Method for detecting contents of retinol and six retinol derivatives in cosmetics
  • Method for detecting contents of retinol and six retinol derivatives in cosmetics

Examples

Experimental program
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Effect test

Embodiment 1

[0074] 1. Standard stock solution

[0075] Take retinol and 6 kinds of retinol derivatives (retinol, retinal, retinyl acetate, retinyl propionate, retinyl palmitate, hydroxy pinacolone retinoate and retinyl retinoic acid ester) standard amount, accurate to 0.0001g, placed in the same 25mL volumetric flask, dissolved with tetrahydrofuran-containing methanol solution, then dilute to the mark, shake well, and make a mixed standard stock solution.

[0076]At the same time, take an appropriate amount of standard products of retinol and 6 kinds of retinol derivatives, accurate to 0.0001g, put them in seven 25mL volumetric flasks respectively, dissolve them with methanol solution containing tetrahydrofuran, adjust the volume to the mark, shake well, Dubbed 7 single standard stock solutions.

[0077] In the methanol solution containing tetrahydrofuran, the volume ratio of tetrahydrofuran to methanol is 2:23. Store the mixed standard stock solution and the 7 single standard stock sol...

Embodiment 2

[0085] 1. Preparation of the test solution

[0086] Put 1g of cosmetic sample (accurate to 0.1mg) in a 25mL stoppered colorimetric tube, add 5mL of tetrahydrofuran, vortex for 1min to disperse, add methanol to the volume, ultrasonically extract for 15min, and take part of the liquid to filter through a 0.22μm filter membrane , to obtain the test solution 1#.

[0087] 2. UV measurement

[0088] Get 7 kinds of single reference substance solutions in Example 1, adopt ultraviolet absorption spectrometry to measure in full scan in the wavelength range of 200-800nm. Detect and obtain the maximum absorption wavelength λmax=325nm of retinol; the maximum absorption wavelength λmax=380nm of retinal; the maximum absorption wavelength λmax=325nm of retinyl acetate; the maximum absorption wavelength λmax= of retinol propionate 325nm; maximum absorption wavelength λmax of retinyl retinoate = 340nm; maximum absorption wavelength λmax = 325nm of retinyl palmitate; maximum absorption wavelen...

Embodiment 3

[0100] 1. Preparation of the test solution

[0101] Put 1g of cosmetic sample (accurate to 0.1mg) in a 25mL stoppered colorimetric tube, add 4mL of tetrahydrofuran, vortex for 1.5min to disperse, add methanol to the volume, ultrasonically extract for 12min, and take part of the liquid through a 0.22μm filter membrane Filter to obtain the test solution 2#.

[0102] 2. UV measurement

[0103] The determination of the maximum absorption wavelengths of retinol and 6 kinds of retinol derivatives is the same as Step 2 of Example 2.

[0104] 3. HPLC determination

[0105] Adopt high performance liquid chromatography (HPLC) to detect respectively the mixed reference substance solution of a series of different concentrations in need testing solution 2# and embodiment 1, be detection wavelength with the maximum absorption wavelength that UV measures, compare retention time and carry out qualitative, adopt Quantification by external standard method. That is, a series of mixed referen...

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Abstract

The invention provides a method for detecting the content of retinol and six retinol derivatives in cosmetics, which comprises the following steps: adding a cosmetic sample into a first solvent for dispersion, then adding a second solvent for ultrasonic extraction, filtering, taking filtrate to obtain a test solution, and determining the detection wavelength by adopting an ultraviolet absorption spectrometry, and carrying out qualitative and quantitative detection by adopting a high performance liquid chromatography to determine the contents of the retinol and the six retinol derivatives in the test solution. The method for detecting the content of the retinol and the six retinol derivatives in the cosmetics has the characteristics of simplicity and convenience in pretreatment, reliability in sample separation, accuracy in quantification and qualitation and the like, and is good in detection recovery rate, high in accuracy, high in sensitivity and low in detection limit.

Description

technical field [0001] The invention belongs to the technical field of chemical component detection in cosmetics, and relates to a detection method for the content of retinol and six retinol derivatives in cosmetics. Background technique [0002] Skin is an important organ to maintain human life and health. However, with age and the influence of external factors, skin will gradually begin to age. With the research and development of cosmetic science and the increasing demand of consumers for the efficacy of cosmetics, anti-aging skin care has become one of the most important consumer demands. [0003] Retinol (vitamin A) is an indispensable and important substance in life activities and a kind of micronutrients necessary for the human body. play an important role. Retinol is a fat-soluble vitamin that is unstable to light, heat, acid, and alkali, and is easily oxidized. It can be oxidized and destroyed under ultraviolet light. Retinol esters can be produced by chemical re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N30/02G01N30/74
Inventor 闫秀芳鲍熹珺陈燕韩小杰严晓娟韩兴艳
Owner 星智豫美(上海)生物科技有限公司