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Production method of feed-grade high-yield aminobutyric acid

A technology of aminobutyric acid and production method, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of increasing feed amount, high conversion rate, and improving cell permeability

Pending Publication Date: 2022-04-15
MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] One of the objectives of the present invention is to propose a feed-grade high-yield GABA production method, which solves the technical problems in the prior art of using chemical synthesis, plant enrichment, and microbial fermentation methods to prepare GABA

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for producing feed-grade high-yield aminobutyric acid, comprising the steps of:

[0040] Step 1, strain activation: take out the preserved strain Escherichia coli BL21, and carry out activation culture on LB plate medium at 37° C. for 24 hours.

[0041] Step 2, strain preparation: the strain was inoculated into the TB shake flask culture medium, and cultured for 12 hours at 37° C., pH value 7.0-8.0, and shake flask rotation speed 220 rpm.

[0042] Step 3, seed culture: inoculate the strains into the seed tank according to the inoculum amount of 0.4%, at 37°C, the tank pressure is 0.05MPa, the air ratio is 1:0.5 (V / V), the dissolved oxygen is 30-40%, and the pH value is 6.5- 7.0, under the condition that the stirring speed is 120-150 rpm, cultivate for 4-6 hours.

[0043] Step 4, bacterial cell culture: the initial ventilation volume is 1:0.5 (V / V), the stirring speed is 80rpm, the tank pressure is 0.05MPa, the temperature is controlled at 36.5-37.5°C, the pH i...

Embodiment 2

[0052] A method for producing feed-grade high-yield aminobutyric acid, comprising the steps of:

[0053] Step 1, strain activation: take out the preserved strain Escherichia coli BL21, and carry out activation culture on LB plate medium at 37° C. for 24 hours.

[0054] Step 2, strain preparation: the strain was inoculated into the TB shake flask culture medium, and cultured for 12 hours at 37° C., pH value 7.0-8.0, and shake flask rotation speed 220 rpm.

[0055] Step 3, seed culture: inoculate the strains into the seed tank according to the inoculum amount of 0.4%, at 37°C, the tank pressure is 0.05MPa, the air ratio is 1:0.5 (V / V), the dissolved oxygen is 30-40%, and the pH value is 6.5- 7.0, under the condition that the stirring speed is 120-150 rpm, cultivate for 4-6 hours.

[0056] Step 4, bacterial cell culture: the initial ventilation volume is 1:0.75 (V / V), the stirring speed is 100rpm, the tank pressure is 0.05MPa, the temperature is controlled at 36.5-37.5°C, the pH...

Embodiment 3

[0065] A method for producing feed-grade high-yield aminobutyric acid, comprising the steps of:

[0066] Step 1, strain activation: take out the preserved strain Escherichia coli BL21, and carry out activation culture on LB plate medium at 37° C. for 24 hours.

[0067] Step 2, strain preparation: the strain was inoculated into the TB shake flask culture medium, and cultured for 12 hours at 37° C., pH value 7.0-8.0, and shake flask rotation speed 220 rpm.

[0068] Step 3, seed culture: inoculate the strains into the seed tank according to the inoculum amount of 0.4%, at 37°C, the tank pressure is 0.05MPa, the air ratio is 1:0.5 (V / V), the dissolved oxygen is 30-40%, and the pH value is 6.5- 7.0, under the condition that the stirring speed is 120-150 rpm, cultivate for 4-6 hours.

[0069] Step 4, bacterial cell culture: the initial gas volume is 1:1 (V / V), the stirring speed is 120rpm, the tank pressure is 0.05MPa, the temperature is controlled at 36.5-37.5°C, the pH is control...

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Abstract

The invention discloses a production method of feed-grade high-yield aminobutyric acid, relates to the technical field of aminobutyric acid production, and solves the technical problems existing in preparation of the aminobutyric acid by adopting a chemical synthesis method, a plant enrichment method and a microbial fermentation method in the prior art. The production method of the feed-grade high-yield aminobutyric acid comprises the following steps of strain activation, strain preparation, seed culture, thallus culture, induction culture, thallus collection, freezing, enzymatic conversion, filtration decoloration and concentration crystallization. According to the production method of the feed-grade high-yield aminobutyric acid, the seed culture method is simple, and the fermentation process is simplified; the cost can be greatly reduced, the enzyme generation and cell permeability are improved through collection and freezing treatment, the feeding amount is greatly increased, the L-glutamic acid is converted to be 2-3 times of the fermentation volume (v / w), and the converted aminobutyric acid can be up to 400-500g / L; enzymatic conversion is thorough, and the conversion rate reaches 99%; the product is simple to extract and the purity is more than or equal to 99%.

Description

technical field [0001] The invention relates to the technical field of aminobutyric acid production, in particular to a method for producing feed-grade high-yield aminobutyric acid. Background technique [0002] γ-Aminobutyric acid (GABA) is a naturally occurring functional non-protein amino acid, which has physiological functions such as improving brain activity, lowering blood pressure, anti-anxiety, regulating hormone secretion, treating epilepsy and protecting liver and kidney. Health care, beverage processing, cosmetics, feed and other fields have broad application prospects and market demands. The preparation methods of γ-aminobutyric acid mainly include chemical synthesis, plant enrichment and microbial fermentation. The chemical synthesis method has harsh conditions, high energy consumption, high cost, low yield and poor safety, so it cannot be used in food, medicine and other fields. The GABA content obtained by the plant enrichment method is low, which is not sui...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C12R1/19
Inventor 杨毅杨发祥尚海丽赵崇钧郭绍青张海鹍
Owner MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE