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Zizania latifolia pollen germination method

A technology of pollen germination and water jasmine, which is applied in the fields of botanical equipment and methods, chemicals for biological control, plant growth regulators, etc., can solve problems such as difficult germination, and achieve the effect of improving germination rate

Active Publication Date: 2022-04-22
JINHUA ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a method for germination of wild rice pollen, aiming to solve the problem that wild rice pollen is not easy to germinate in vitro culture in the prior art

Method used

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  • Zizania latifolia pollen germination method
  • Zizania latifolia pollen germination method
  • Zizania latifolia pollen germination method

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Embodiment 1

[0024] The present embodiment provides a method for germination of Zizania zizania pollen, comprising the following steps:

[0025] Step 1: Pick the male and female flowers of Zizania zizania and put them in a petri dish, cover the petri dish with the lid of the petri dish and put it in an incubator at 30-35°C for 50-70 minutes; the best temperature of the incubator is 32 °C, the standing time can be 60 minutes.

[0026] The glume of the female flower can be removed and the stigma can be kept, and then placed in a petri dish; when selecting the female flower, it is preferred to come from a different plant, and the picked female flower is the female flower in the pollination stage. The best picking time of the Zizania male flowers is before 7 o'clock in the morning of the powder loosening day.

[0027] Step 2: Collect the pollen from the male flowers of Zizania zizania, and sprinkle it in the germination agent of Zizania zizania pollen; the germination agent of Zizania zizania...

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Abstract

The invention provides a Zizania aquatica pollen germination method, and aims to solve the problem that Zizania aquatica pollen is not easy to germinate in vitro culture in the prior art. The zizania latifolia pollen germination method comprises the following steps: picking zizania latifolia male flowers and zizania latifolia female flowers, putting the picked zizania latifolia male flowers and zizania latifolia female flowers into a culture dish, covering the culture dish with a culture dish cover, putting the culture dish into a 30-35 DEG C incubator, collecting pollen in the staminate flowers of zizania aquatica, and scattering the pollen in the pollen germinating agent of zizania aquatica; the zizania latifolia pollen germinating agent is prepared from 5.5 parts by mass of potato starch, 18 parts by mass of cane sugar, 0.008 part by mass of boric acid, 10 parts by mass of PEG4000, 0.05 part by mass of Ca (NO3) 2 and 66.442 parts by mass of distilled water. And putting the zizania aquatica pollen germinating agent with scattered pollen into an incubator at 28 DEG C, and culturing for 30-35 minutes. By adopting the zizania latifolia pollen germinating agent and the germination method, the zizania latifolia pollen can germinate.

Description

technical field [0001] The invention belongs to the technical field of Zizania hybridization, and in particular relates to a method for germination of Zizania pollen. Background technique [0002] Zizania is a vegetatively propagated crop and does not flower under normal circumstances. Breeding of Zizania zizania varieties usually adopts the method of systematic breeding, selecting excellent single plants from natural mutants for breeding. Due to the low rate of natural variation and the small number of excellent variations, the selection and breeding of Zizania zizazaza have high randomness and low efficiency. At present, we have developed a method of inducing Zizania to flower, and further application of cross-directed breeding technology may be the key to breaking this bottleneck. Pollen activity is an important factor affecting hybrid breeding. Zizania pollen itself has low activity, and its activity will further decrease after low-temperature storage. Therefore, confi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01N59/14A01N59/06A01N43/16A01N25/00A01P21/00
CPCA01H1/02A01N59/14A01N59/06A01N43/16A01N25/00
Inventor 杨梦飞李怡鹏王凌云石林王涛张尚法郑寨生
Owner JINHUA ACAD OF AGRI SCI