Polyphenol and antibacterial peptide combined fibrous membrane as well as preparation method and application thereof
A fibrous membrane and antibacterial peptide technology, applied in the field of fibrous membrane, can solve the problem of inability to achieve H-type vascularized bone tissue regeneration, and achieve the effects of reducing cytotoxicity, facilitating cell proliferation, and promoting bone tissue regeneration.
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Embodiment 1
[0045] A method for preparing a fibrous membrane combined with polyphenols and antibacterial peptides. The raw materials include polylactic acid-glycolic acid from Shandong Jinan Daigang Biotechnology Co., Ltd., fish collagen from Sangon Biotechnology Co., Ltd., tannic acid from Aladdin Company and Hexafluoroisopropanol, LL-37 peptide from Nanjing Jietai Biotechnology Co., Ltd., the specific steps are as follows:
[0046] Step 1: Weigh 0.04g of fish collagen and disperse it in 1mL of hexafluoroisopropanol, weigh 0.2g of polylactic acid-glycolic acid and add it to the obtained dispersion, shake it with a constant temperature oscillator for 60min to obtain a spinning solution, pass Fish collagen / polylactic acid-glycolic acid fiber membrane was prepared by electrospinning. The spinning voltage was 8kV, the injection speed was 0.5mL / h, and the receiving distance was 15cm. The electrospun fiber membrane was dried in a vacuum drying oven for one week;
[0047] Step 2: Soak the fiber...
Embodiment 2
[0051] A method for preparing a fiber membrane combined with polyphenols and antibacterial peptides. The raw materials include polylactic acid-glycolic acid from Shandong Jinan Daigang Biotechnology Co., Ltd., polycaprolactone from Shenzhen Guanghua Weiye Co., Ltd., and Sangon Biological Co., Ltd. Fish collagen, tannic acid, baicalin, hexafluoroisopropanol and trifluoroethanol from Aladdin Company, LL-37 peptide from Nanjing Jietai Biotechnology Co., Ltd., the specific steps are as follows:
[0052] Step 1: Weigh 0.015g baicalin and disperse it in 1mL trifluoroethanol, weigh 0.12g polycaprolactone and add it to the obtained dispersion, shake it with a constant temperature oscillator for 45min to obtain spinning solution A, weigh Disperse 0.08g of fish collagen in 2mL of hexafluoroisopropanol, weigh 0.4g of polylactic acid-glycolic acid and add it to the obtained dispersion, and shake it with a constant temperature oscillator for 60min to obtain spinning solution B;
[0053] St...
Embodiment 3
[0058] A method for preparing a fiber membrane combined with polyphenols and antibacterial peptides. The raw materials include polylactic acid-glycolic acid from Shandong Jinan Daigang Biotechnology Co., Ltd., polycaprolactone from Shenzhen Guanghua Weiye Co., Ltd., and Sangon Biological Co., Ltd. Fish collagen, tannic acid, baicalin, hexafluoroisopropanol and trifluoroethanol from Aladdin Company, LL-37 peptide from Nanjing Jietai Biotechnology Co., Ltd., the specific steps are as follows:
[0059] Step 1: Weigh 0.015g baicalin and disperse it in 1mL trifluoroethanol, weigh 0.12g polycaprolactone and add it to the obtained dispersion, shake it with a constant temperature oscillator for 45min to obtain spinning solution A, weigh Disperse 0.08g of fish collagen in 2mL of hexafluoroisopropanol, weigh 0.4g of polylactic acid-glycolic acid and add it to the obtained dispersion, and shake it with a constant temperature oscillator for 60min to obtain spinning solution B;
[0060] St...
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