Production method of selenium-enriched yeast with high biomass

A selenium-enriched yeast, high-biomass technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, yeast-containing food ingredients, etc. The problem of single type of selenium yeast can achieve the effect of increasing the conversion rate, enriching nutrients and reducing the processing cost.

Pending Publication Date: 2022-04-29
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Description
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  • Application Information

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Problems solved by technology

[0004] In recent years, the research on selenium-enriched yeast has progressed rapidly, which has the advantages of fast reproduction and high material utilization rate. At present, most of the literature reports on selenium-enriched yeast are Saccharomyces cerevisiae; Sodium selenite is used to cultivate selenium-enriched yeast under certain culture conditions, and is

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  • Production method of selenium-enriched yeast with high biomass
  • Production method of selenium-enriched yeast with high biomass
  • Production method of selenium-enriched yeast with high biomass

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0069] Example 1

[0070] The preparation method of the selenium-enriched yeast of this example is as follows:

[0071] (1) Screening and identification of selenium-enriched yeast:

[0072] The selenium-enriched soil was taken from a pear orchard in Liujun Town, Huaian City, at a distance of 3-5 cm from the surface; an appropriate amount of selenium-enriched soil was placed in the enriched medium, and cultured at 30°C and 200 rpm / min for 1-2 days to obtain a pre-medium culture. Under sterile conditions, take 1 mL of the above-mentioned enriched mother liquor respectively, and use sterile normal saline to dilute to 10 by 10-fold gradient dilution method. -1 -10 -6 Gradient, get sample homogeneous solution; choose 10 -3 ~10 -6 Dilute gradient sample homogenate, draw 200 μL of each dilution gradient and spread it evenly on the sterilized purification plate, then place it in a constant temperature incubator for 48-72 hours, observe and record the colony characteristics; The c...

Example Embodiment

[0090] Example 2

[0091] The preparation method of the selenium-enriched yeast of the present embodiment is as follows:

[0092] (1) Screening and identification of selenium-enriched yeast

[0093] The screening and identification of selenium-enriched yeast are the same as in Example 1.

[0094] (2) Mutagenesis and breeding of selenium-enriched yeast

[0095] The mutagenesis breeding of selenium-enriched yeast is the same as in Example 1.

[0096] (3), Shake flask medium optimization

[0097] Shake flask medium optimization is the same as Example 1.

[0098] (4), fermentation process control:

[0099] According to the obtained medium composition and selenium-enriched conditions, a continuous culture method is adopted, and the production process includes the following steps in turn: slanted bacteria, primary seed culture, secondary seed culture, fermentation culture, collection of bacterial cells and spray drying, packaging, packaging , the production processes are as fo...

Example Embodiment

[0107] Example 3

[0108] The preparation method of the selenium-enriched yeast of the present embodiment is as follows:

[0109] (1) Screening and identification of selenium-enriched yeast

[0110] The screening and identification of selenium-enriched yeast are the same as in Example 1.

[0111] (2) Mutagenesis and breeding of selenium-enriched yeast

[0112] The mutagenesis breeding of selenium-enriched yeast is the same as in Example 1.

[0113] (3), Shake flask medium optimization

[0114] Shake flask medium optimization is the same as Example 1.

[0115] (4), fermentation process control:

[0116] According to the obtained medium composition and selenium-enriched conditions, a continuous culture method is adopted, and the production process includes the following steps in turn: slanted bacteria, primary seed culture, secondary seed culture, fermentation culture, collection of bacterial cells and spray drying, packaging, packaging , the production processes are as fo...

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Abstract

The invention discloses selenium-enriched yeast with high biomass and a preparation method of the selenium-enriched yeast. Belongs to the field of microbial breeding and fermentation engineering. The method comprises the following specific steps: taking soil which is 3-5cm away from a surface layer, performing enrichment culture, and performing gradient dilution and purification to obtain relatively pure saccharomycetes; the preparation method comprises the following steps: breeding a selenium-rich yeast strain with high biomass and high selenium content by virtue of a plasma mutation breeding technology, optimizing optimal fermentation medium composition and fermentation conditions, performing primary seed culture, secondary seed culture and fed-batch fermentation culture, feeding sodium selenite within about 10 hours, collecting fermentation liquor, and performing centrifugal spray drying, so as to prepare faint yellow dry powder; and finally, the yeast with high selenium and high biomass is prepared. The selenium-enriched yeast produced by the invention has high protein content and high nucleic acid content, and is easy to absorb and convert by organisms; the high-biomass selenium-enriched yeast prepared by the invention has the characteristics of low cost, safety, no toxicity, simplicity in operation, large biomass and the like.

Description

technical field [0001] The invention belongs to the field of microbial breeding and fermentation engineering, and relates to the screening of a high-biomass, high-selenium-enriched yeast strain and the optimization of the condition process. Background technique [0002] Selenium (Se) is one of the important trace elements to maintain the normal physiological activities of humans or animals. Selenium is also a multifunctional life nutrient, which is often used for Keshan disease, Kashin-Beck disease, cardiovascular disease, diabetes, liver disease, and prostate disease. , heart disease, cancer and more than 40 kinds of diseases, widely used in cancer, surgery, radiotherapy and chemotherapy, etc. Higher animals can use selenium to participate in their own growth metabolism and immune regulation, but they cannot convert inorganic selenium into organic selenium. [0003] How to convert unusable inorganic selenium into organic selenium that can be absorbed by the human body gener...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/02C12N1/38C12N13/00C12N15/01C12N1/04A23L31/10A23L33/135A23K10/18C12R1/84
CPCC12N1/16C12N1/02C12N1/38C12N13/00C12N15/01C12N1/04A23L31/10A23L33/135A23K10/18A23V2002/00A23V2250/76A23V2200/30
Inventor 李相前李明振丁浩聂新玲杨宇翔李青飞信姗姗李婧袁稚雯
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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