Unlock instant, AI-driven research and patent intelligence for your innovation.

Detection method and kit for diagnosing staphylococcus aureus blood flow infection

A staphylococcus and detection method technology, applied in the field of nucleic acid detection, can solve the problems such as the circular RNA kit for detecting Staphylococcus aureus bloodstream infection, and achieve the effects of shortening detection time, saving detection cost and being expensive.

Pending Publication Date: 2022-04-29
GENERAL HOSPITAL OF NUCLEAR IND
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no circular RNA kit for the detection of Staphylococcus aureus bloodstream infection using PCR technology

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method and kit for diagnosing staphylococcus aureus blood flow infection
  • Detection method and kit for diagnosing staphylococcus aureus blood flow infection
  • Detection method and kit for diagnosing staphylococcus aureus blood flow infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Composition and detection method of a kit for rapid detection of Staphylococcus aureus bloodstream infection-specific circular RNA

[0036] 1. The composition of the kit

[0037] (1) Primer set: for the detection of Staphylococcus aureus bloodstream infection-specific circular RNA, the amplification primer pair of hsa-circ-0000311 includes the addition of the 3' end of the PCR product according to the principle of Poly(A) tailing method An "A", so it can be connected with a primer with a prominent "T" The characteristically designed specific primer hsa-circ-0000311-F and the universal downstream primer.

[0038] The amplification primer pair of hsa-circ-0000311 gene is:

[0039] hsa-circ-0000311-F (SEQ ID NO: 1): 5'-AGCTGAACCTCTATGACCGC-3';

[0040] hsa-circ-0000311-R (SEQ ID NO: 2): 5'-GAATCTGTTGGGCTGTCACC-3'.

[0041] PCR primers were synthesized by Suzhou Gemma Gene Co., Ltd.

[0042] (2) The preparation process of the kit

[0043] Prepare 20 μL PCR reaction syste...

Embodiment 2

[0052] The method for detecting the specific circular RNA of Staphylococcus aureus bloodstream infection using the kit described in Example 1.

[0053] 1. Sample

[0054] Collect normal human serum clinical samples, then use the method described in Example 1 to obtain cDNA, and make it into a negative cDNA sample. The clinical samples used in this example were serum samples from patients with Staphylococcus aureus bloodstream infection.

[0055] 2. Detection of Staphylococcus aureus bloodstream infection-specific circular RNA, hsa-circ-0000311 in serum

[0056] First prepare the PCR reaction solution according to Table 1 in Example 1, set up 3 parallels, and divide into 2mL reaction tubes, and add the template to be tested respectively, cover the tube cap and put it into the Applied Biosystems StepOnePlus96 fluorescence real-time PCR instrument, using The following reaction program was used for PCR reaction: 95°C, 10min, 95°C, 10s, 60°C, 30s, 40 cycles. The real-time fluore...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a detection method for diagnosing staphylococcus aureus bloodstream infection and a kit thereof, the detection method can rapidly, efficiently and accurately detect specific circular RNA of staphylococcus aureus bloodstream infection serum, the detection time is greatly shortened, the detection cost is saved, and the detection efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of nucleic acid detection, more specifically, it relates to a detection method for diagnosing Staphylococcus aureus bloodstream infection and a kit thereof. Background technique [0002] Staphylococcus aureus is a common gram-positive bacterium that causes community and hospital-acquired infections ranging from mild skin and soft tissue infections to severe bloodstream infections including bacteremia, sepsis, pus Toxemia and osteomyelitis, etc., have high morbidity and mortality. The results of the study proved that the occurrence and evolution of Staphylococcus aureus bloodstream infection are closely related to gene mutation and expression level. Therefore, studying the underlying gene molecular expression mechanism of Staphylococcus aureus bloodstream infection and searching for potential biomarkers and therapeutic targets is crucial for the early diagnosis of the disease and the proposal of new treatmen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6883C12Q1/689C12Q1/6851C12N15/11C12N15/113C12R1/445
CPCC12Q1/6883C12Q1/689C12Q1/6851C12N15/113C12Q2600/178C12Q2600/158C12N2310/532C12Q2531/113C12Q2545/101C12Q2563/107C12Q2525/173
Inventor 张海方解晓露陈志豪韩明霄杜鸿王敏谢小芳
Owner GENERAL HOSPITAL OF NUCLEAR IND