Detection method for hemagglutination titer of influenza virus antigen sample
An influenza virus and detection method technology, applied in the biological field, can solve problems such as inability to control and evaluate the quality, and achieve the effects of improving detection accuracy, improving stability, and eliminating influence.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example 1
[0086] Preparation Example 1 provides a H1N1 influenza virus hemagglutination titer reference product, the preparation steps of which are:
[0087] S1. Add 30 mL of water to the container, heat to boil, add 10 g of maltose weighed, stir until completely dissolved, add water and phosphate to prepare a solution of 0.1 g / mL and pH 7.4 to obtain a lyoprotectant;
[0088] S2. Take the monovalent stock solution of H1N1 influenza virus split vaccine (taken from the company's production workshop), and dilute it with 0.02mol / LPBS buffer solution to a dilution with a hemagglutinin content of 42 μg / mL and a hemagglutination titer of 1:768 solution, to obtain dilution A;
[0089] S3. Take 200 mL of the diluent A obtained in the step S2 and add the lyoprotectant obtained in the step S1 to obtain a reference solution with a hemagglutinin content of 28 μg / mL and a hemagglutination titer of 1:512;
[0090] S4. Divide the reference product solution obtained in step S3 into freeze-dried bottle...
preparation example 2
[0101] Preparation Example 2 provides a H3N2 influenza virus hemagglutination titer reference product. The difference between the preparation steps and Preparation Example 1 is that in step S2, the H1N1 influenza virus split vaccine monovalent stock solution is replaced by the H3N2 influenza virus split vaccine Monovalent stock solution.
[0102] Preparation Example 2 provides a H3N2 influenza virus hemagglutination titer reference product. The difference between the preparation steps and Preparation Example 1 is that the H1N1 influenza virus split vaccine monovalent stock solution is replaced by the H3N2 influenza virus split vaccine monovalent stock solution; T of H3N2 influenza virus hemagglutination titer reference product 0 1:503.
preparation example 3
[0103] Preparation Example 3 provides a BV-type influenza virus hemagglutination titer reference product, and its preparation steps are different from Preparation Example 1 in that:
[0104] S2. Take the monovalent stock solution of the BV-type influenza virus split vaccine (taken from the production workshop of our company), and dilute it with 0.02mol / L PBS buffer solution to a hemagglutinin content of 39 μg / mL and a hemagglutination titer of 1:2048. Diluent, get diluent A;
[0105] S3. Take 200 mL of the diluent A obtained in the step S2 and add the lyoprotectant obtained in the step S1 to obtain a reference solution with a hemagglutinin content of 26 μg / mL and a hemagglutination titer of 1:1536;
[0106] T of the BV influenza virus hemagglutination titer reference product 0 for 1:1492.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More