Nursing culture method for immature seeds of torreya grandis
A cultivation method and immature technology, applied in the field of care and cultivation of immature seeds of Torreya dapanshan, can solve the problems affecting the normal development of embryos, browning, etc., and achieve the effect of low pollution rate and strong operability
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[0025] Example 1:
[0026] Material: This example uses the immature seeds of Dapanshan beech as the starting material for cultivation.
[0027] Step (1): Material extraction
[0028]From late July to late August, the immature seeds of Dapanshan were collected in the Dapanshan National Nature Reserve in Pan'an County, Zhejiang Province. Figure 1 )。 Immature seeds are green and ovoid in shape. Can be stored in a sealed bag and stored at a low temperature of 4 °C.
[0029] Step (2): Seed surface sterilization
[0030] Wipe the seed surface with 75% ethanol and remove the wrapped turquoise pseudofond with forceps. On the ultra-clean bench, soak with 70% ethanol aqueous solution and shake for 30s, rinse with sterile water 3 times for 1 min each time. After soaking and shaking with an aqueous solution of mercuric chloride with a mass fraction of 0.1%, rinse with sterile water 5 times for 1 min each time. Finally, use sterile filter paper to absorb excess water from the surface.
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