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Lipid metabolism marker composition for detecting acute myocardial infarction, kit and application

A technology for acute myocardial infarction and lipid metabolism, applied in the field of serum biological markers, can solve the problems of hidden acute myocardial infarction and lack of early diagnostic markers, and achieve accurate identification and diagnosis

Pending Publication Date: 2022-05-13
李继承
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The onset of acute myocardial infarction is generally hidden, and the current diagnostic window is mostly within 2-4 hours, and there is still a lack of effective early diagnostic markers

Method used

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  • Lipid metabolism marker composition for detecting acute myocardial infarction, kit and application
  • Lipid metabolism marker composition for detecting acute myocardial infarction, kit and application
  • Lipid metabolism marker composition for detecting acute myocardial infarction, kit and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1 The composition of the early diagnosis kit of acute myocardial infarction based on plasma lipid metabolites

[0073] The tuberculosis curative effect evaluation kit based on plasma lipid metabolism composition, including various reagents used in liquid chromatography, including methanol, acetonitrile, acetic acid, ammonium formate, chloroform and methyl tert-butyl ether, which are of chromatographically pure grade, the All reagents can be purchased from Merc.

[0074] FFA ((20:1)), CarnitineC18:1-OH, and CarnitineC18:2-OH standard products are all chromatographically pure brands and can be purchased from BioBioPha / Sigma-Aldrich. The three standard substances were respectively dissolved in dimethyl sulfoxide (DMSO) or methanol as a solvent and stored at -20°C. Diluted with 70% methanol to different gradient concentrations before mass spectrometry analysis.

Embodiment 2

[0075] Example 2 Using the kit for detecting acute myocardial infarction to perform ROC curve analysis on plasma samples

[0076] The acute myocardial infarction early screening kit was used to detect the expression levels of FFA((20:1)), CarnitineC18:1-OH and CarnitineC18:2-OH in the plasma of healthy controls, patients with stable angina pectoris and patients with acute myocardial infarction. Among them, the plasma samples of healthy controls, patients with stable angina pectoris, and patients with acute myocardial infarction came from the People's Hospital of North Guangdong.

[0077] 1. Sample Collection

[0078] Heparin anticoagulated plasma samples were collected from 60 cases of acute myocardial infarction (untreated at first diagnosis), 60 patients with stable angina pectoris (uncured at first diagnosis), and 30 healthy controls. Patients with acute myocardial infarction and stable angina were diagnosed according to the 2020 version of the guidelines for the diagnosis...

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Abstract

The invention provides a lipid metabolism marker composition for detecting acute myocardial infarction, a kit and application. The lipid metabolism marker composition for detecting acute myocardial infarction consists of free fatty acid and acyl carnitine, the free fatty acid is eicosaenoic acid FFA (20: 1); the acyl carnitine is prepared from 1-hydroxy octadecenoyl carnitine Carnitine C18: 1-OH and 2-hydroxy octadecenoyl carnitine Carnitine C18: 2-OH, and the acylcarnitine is prepared from 1-hydroxy octadecenoyl carnitine Carnitine C18: 1-OH and 2-hydroxy octadecenoyl carnitine Carnitine C18 And the acute myocardial infarction can be accurately and early identified and diagnosed by detecting lipid metabolites of a human plasma sample. Therefore, a new objective method is provided for early screening and diagnosis of acute myocardial infarction.

Description

technical field [0001] The present application relates to the technical field of serum biological markers, in particular to a lipid metabolism marker composition, kit and application for detecting acute myocardial infarction. Background technique [0002] Acute myocardial infarction has a high morbidity and mortality rate worldwide. In 2016, the WHO report showed that the number of deaths due to myocardial infarction accounted for 31% of the global death toll due to the disease. According to incomplete statistics, about 10% of emergency chest pain patients are finally diagnosed with myocardial infarction every year. In the past ten years, the number of patients admitted to hospital due to acute myocardial infarction in my country has increased by more than four times. The mortality rate of acute myocardial infarction in my country also shows a rapid growth trend. From 2002 to 2015, the mortality rate of acute myocardial infarction in rural areas in my country increased from...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86
CPCG01N30/8634G01N30/8675G01N30/8696
Inventor 李继承刘俊
Owner 李继承