Unlock instant, AI-driven research and patent intelligence for your innovation.

Method and device for preparing long-chain dicarboxylic acid through fermentation

A long-chain dibasic acid and fermentation bacteria technology, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of increasing the difficulty of refining long-chain dibasic acids, the incomplete precipitation of dibasic acids, and the reduction of dibasic acids. It can avoid the production of high-salt wastewater, be easy for industrial application, and improve the fermentation level.

Active Publication Date: 2022-05-24
CHINA PETROLEUM & CHEM CORP +1
View PDF13 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this patent does not use sulfuric acid, in order to obtain the solid long-chain dibasic acid sodium salt, a high-energy distillation concentration process is required, and the small molecular weight impurity components in the fermentation broth and the long-chain dibasic acid sodium salt are precipitated at the bottom of the still , thus increasing the difficulty of refining long-chain dibasic acids in the later stage
In addition, organic acids are weak acids, and dibasic acids cannot be completely precipitated during the acid precipitation process. Compared with the sulfuric acid refining scheme, the content of dibasic acids in the crystallization mother liquor is higher, which will lead to the loss of some dibasic acids and reduce the yield of dibasic acids

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and device for preparing long-chain dicarboxylic acid through fermentation
  • Method and device for preparing long-chain dicarboxylic acid through fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Fermentation medium formula: sucrose 20g / L, corn steep liquor 2g / L, yeast extract 1.5g / L, sodium chloride 1.2g / L, potassium dihydrogen phosphate 3.5g / L, magnesium sulfate 1.8g / L, urea 4g / L, ammonium sulfate 1.5g / L.

[0042] Seed liquid preparation: Inoculate 50 mL of fermented bacteria liquid into a 3L shaker flask containing 450 mL of seed medium, at a temperature of 32°C, with a shaker revolution of 120 rpm, and cultured for 48 hours to obtain a fermented bacteria seed liquid. The fermentation control volume is 5L.

[0043] (1) Inoculate 500 mL of fermentation bacteria seed liquid into 3.25 L fermentation medium, and then add 1.25 L dodecane. The fermentation temperature was 32° C., the stirring revolution was 400 rpm, and the aeration was set to 0.8 vvm. From 24h fermentation, 5mol / L sodium acetate solution was added at a rate of 4mL / h, and a total of 480mL was added.

[0044] (2) After the fermentation, the fermentation broth was continuously heated to 90°C and ...

Embodiment 2

[0048] The fermentation medium formula is: sucrose 10g / L, corn steep liquor 2g / L, yeast extract 1.5g / L, sodium chloride 0.8g / L, potassium dihydrogen phosphate 3.5g / L, magnesium sulfate 1.2g / L, urea 4g / L, ammonium sulfate 1.5g / L.

[0049] Seed liquid preparation: inoculate 60 mL of fermented bacteria liquid into a 3L shaker flask containing 540 mL of seed medium, at a temperature of 29° C., with a shaker revolution of 120 rpm, and cultured for 48 hours to obtain a fermented bacteria seed liquid. The fermentation control volume is 6L.

[0050] (1) Inoculate 600 mL of fermented bacteria seed liquid into 4.2 L of fermentation medium, and then add 1.20 L of dodecane. The fermentation temperature was 29°C, the stirring revolution was 400 rpm, and the aeration was set to 1.0 vvm. Starting from the fermentation for 24 h, 4 mol / L sodium acetate solution was added at a rate of 5 mL / h, for a total of 600 mL.

[0051] (2) After the fermentation, the fermentation broth was continuously...

Embodiment 3

[0055] Fermentation medium formula: sucrose 20g / L, corn steep liquor 2g / L, yeast extract 1.5g / L, sodium chloride 1.2g / L, potassium dihydrogen phosphate 3.5g / L, magnesium sulfate 1.8g / L, urea 4g / L, ammonium sulfate 1.5g / L.

[0056] Seed liquid preparation: Inoculate 50 mL of fermented bacteria liquid into a 3L shaker flask containing 450 mL of seed medium, at a temperature of 32°C, with a shaker revolution of 120 rpm, and cultured for 48 hours to obtain a fermented bacteria seed liquid. The fermentation control volume is 5L.

[0057] (1) Inoculate 500 mL of fermentation bacteria seed liquid into 3.25 L fermentation medium, and then add 1.25 L dodecane. The fermentation temperature was 32° C., the stirring revolution was 400 rpm, and the aeration was set to 0.8 vvm. From 24h fermentation, 5mol / L sodium acetate solution was added at a rate of 4mL / h, and a total of 480mL was added.

[0058] (2) After the fermentation, the fermentation broth was continuously heated to 90°C and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The method comprises the following steps: (1) inoculating a zymophyte seed solution into a fermentation culture medium, adding alkane for fermentation, and feeding an organic acid salt solution in an acid production period until the fermentation is finished; (2) demulsifying and filtering the fermentation liquid to obtain a water-phase clear liquid, carrying out acidification treatment by using the organic acid corresponding to the organic acid salt in the step (1), and filtering to obtain a filter cake, namely a long-chain dicarboxylic acid crude product; and (3) dehydrating and concentrating the filtrate after filtering to obtain organic acid salt which is reused in the step (1). The method provided by the invention obviously reduces the dosage of acid and alkali reagents, avoids the generation of high-salinity wastewater, improves the alkane conversion rate, and is beneficial to industrial application.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a method and a device for preparing long-chain dibasic acid by fermentation. Background technique [0002] Long chain dicarboxylic acids (Long chain dicarboxylic acids) refer to aliphatic dicarboxylic acids (DCn) containing more than 10 carbon atoms in the carbon chain, including saturated and unsaturated dicarboxylic acids. Use of fine chemical products. At the same time, it is also an important raw material in the chemical industry to synthesize high-grade fragrances, high-performance nylon engineering plastics, high-grade nylon hot-melt adhesives, high-temperature dielectrics, high-grade paints and coatings, high-grade lubricants, cold-resistant plasticizers, resins, medicines and pesticides. [0003] The production of long-chain dibasic acid by fermentation method utilizes the unique oxidative ability of microorganisms and the action of intracellular enzymes. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/6409C07C51/50C07C55/21C12R1/74
CPCC12P7/6409C07C51/50C07C55/21
Inventor 张霖樊亚超师文静
Owner CHINA PETROLEUM & CHEM CORP