Extraction device for full-automatic nucleic acid extraction, amplification and detection
An extraction device and fully automatic technology, which can be used in biochemical cleaning devices, enzymology/microbiology devices, bioreactors/fermenters for specific purposes, etc. Problems such as small sample size and high cost can increase the detection throughput, simplify the structure, and reduce the volume.
Pending Publication Date: 2022-05-27
杭州天微基因科技有限公司
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Problems solved by technology
The integrated technology of nucleic acid extraction, amplification and detection includes multiple steps such as lysis, cleaning and purification, and amplification detection. Promotion of integrated technology of amplification and detection
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[0031] The technical embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings of the present invention.
[0032] like figure 1 , 4. The present invention relates to an extraction device for automatic nucleic acid extraction, amplification and detection, comprising: a driving wheel (2), a hollow gear (3), a ball (4), and a magnet (10).
[0033] like Figure 9 The hollow gear (3) shown is provided with a hollow gear through hole (301) in the center for placing the test tube (1), and the side wall of the hollow gear (3) is provided with a magnet fixing hole (303) for fixing the magnet (10). ), the rotation of the hollow gear (3) can drive the magnet (10) to move synchronously, and the rotation of the magnet (10) can drive the nano-magnetic beads of the test tube (1) to pass through different functional areas in the test tube. A ball groove (302) is provided on the side, and the ball groove (302) is a hemisph...
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The invention discloses an extraction device for full-automatic nucleic acid extraction, amplification and detection. The extraction device comprises a driving wheel, a magnet, a hollow gear and a ball, balls are arranged between the hollow gear and the fixing cover and between the hollow gear and the fixing seat respectively, a magnet is arranged on the side face of the hollow gear, and ball guide grooves are formed in the fixing cover and the fixing seat respectively. The driving wheel drives the hollow gears to rotate, each hollow gear drives the magnet to rotate around the test tube, the magnet drives the nano magnetic beads in the test tube to rotate, and nucleic acid is adsorbed by the nano magnetic beads and penetrates through different functional areas in the test tube, so that the nucleic acid extraction process is realized. The structure of the nucleic acid extraction, amplification and detection integrated equipment is greatly simplified, the size of the equipment is reduced, the cost of the equipment is reduced, and the detection flux of the nucleic acid extraction, amplification and detection integrated equipment can be greatly improved.
Description
technical field [0001] The invention belongs to the field of molecular diagnosis, and relates to an extraction device for automatic nucleic acid extraction, amplification and detection. Background technique [0002] The principle of nucleic acid extraction by magnetic bead method is based on the same principle as that of silica gel membrane spin column, using nanotechnology to improve and modify the surface of superparamagnetic nanoparticles, and then prepare superparamagnetic silica nanomagnetic beads. The magnetic beads can specifically recognize and efficiently bind to nucleic acid molecules on a microscopic interface. Using the superparamagnetic properties of silica-coated nanomagnetic microspheres, under the action of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate, etc.) and an external magnetic field, samples from blood, animal tissues, food, pathogenic microorganisms, etc. The isolated DNA and RNA can be used in clinical disease diagnosis, blood ...
Claims
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IPC IPC(8): C12M1/34C12M1/00C12Q1/6806C12Q1/6844C12N15/10
CPCC12Q1/6806C12Q1/6844C12N15/1013C12Q2523/308C12Q2563/155C12Q2563/149
Inventor 邵光业谢波郭登五徐志珍尹居鑫其他发明人请求不公开姓名
Owner 杭州天微基因科技有限公司