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Method for determining risk of patient developing hygiene-related infection

A technology for health care and patients, applied in biochemical equipment and methods, microbiological determination/inspection, informatics, etc.

Pending Publication Date: 2022-06-21
BIOMERIEUX SA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the literature, it has been demonstrated that decreased expression of CX3CR1 is associated with increased mortality, especially in patients with a septic state, and more particularly in patients with septic shock (Pachot et al. (2008), J Immunol 180:6421-6429), but the utility of measuring CX3CR1 expression for predicting the occurrence of healthcare-associated infection has never been demonstrated or suggested

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  • Method for determining risk of patient developing hygiene-related infection
  • Method for determining risk of patient developing hygiene-related infection
  • Method for determining risk of patient developing hygiene-related infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Measurement of CX3CR1 expression can predict a patient's risk of developing health care-associated infections

[0041] Materials and methods

[0042] A prospective, longitudinal, and single-center observational clinical study was conducted at the Edouard Herriot Hospital (Lyon, France). The design of this clinical study has been published in Rol et al. (2017), BMJ Open 7(6):e015734. The clinical study was approved by the National Agency for the Safety of Medicines and Health Products (ANSM) in November 2015 and by the Comité de Protection des Personnes SUD-EST II in December 2015. The agreement was amended in July 2016 and January 2017. Briefly, a total of 377 patients and 175 healthy volunteers were included between December 2015 and March 2018, who were in a septic state (n=35) or septic shock (n=72), Suffered severe burns (n=24), severe trauma (n=137) or were admitted to a resuscitation or intensive care unit (n=109) after major surgery.

[0043] - ...

Embodiment 2

[0067] Example 2: Measuring the expression of CX3CR1 and another gene can increase the risk of developing health care-associated infections The predictive performance of

[0068] Materials and methods

[0069] Materials and methods are the same as in Example 1, except that 1) the cDNA pre-amplification step has used another PreAmp master mix reference for specific genes (BTLA and IL15, and reference genes that have been measured with 2 references) PN100-5875C1, Fluidigm) followed by an additional step of exonuclease I treatment (cf. PN100-5875C1, Fluidigm), and in addition to these genes, the amplification step also used another type of Fluidic Integrated Circuits 192.24 (with reference to PN100-7222 C1), and 2) here, a multiple logistic regression (a combination of measures of expression of CX3CR1 and another gene selected from the list consisting of: BTLA) has been performed , CD3D, CD74, CD274, CTLA4, HP, ICOS, IFNG, IL1RN, IL7R, IL10, IL15, PDCD1 and S100A9).

[0070...

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Abstract

The present invention relates to an in vitro or ex vivo method for determining the risk of a patient to develop a hygiene-related infection, comprising the step of measuring the expression of CX3CR1 in a biological sample of said patient.

Description

technical field [0001] The present invention relates to an in vitro or ex vivo method for determining a patient's risk of developing a healthcare-associated infection comprising the step of measuring CX3CR1 expression in a biological sample of said patient. Background technique [0002] The development of healthcare-associated infections is a major complication associated with healthcare, especially in healthcare settings such as hospitals (where we discuss nosocomial infections in more detail). Nosocomial infections in the intensive care unit (occurring in 20% to 40% of patients) have been shown to be associated with increased morbidity and mortality, longer duration of supportive care for organ failure, longer hospital stays, higher health care costs, and antibiotics associated with its high use (and thus lead to antimicrobial resistance). The emergence of health care-associated infections has been particularly severe in recent years due to the rise of multidrug-resistant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/158C12Q2600/16G16H50/30
Inventor 劳伦斯·瓦绍弗朗索瓦·马莱纪尧姆·莫内雷维尔日妮·穆卡德尔亚历山大·帕绍埃斯特尔·佩龙内特托马斯·里梅莱朱利安·特克托里斯法比耶纳·维奈特
Owner BIOMERIEUX SA