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Microbial agent formula for soil improvement and preparation method thereof

A microbial inoculum and soil improvement technology, applied in the directions of microorganism-based methods, methods of using microorganisms, biochemical equipment and methods, etc., can solve the problems of insufficient stability of bacteriostatic performance, achieve stable and reliable bacteriostatic ability, reduce The effect of metabolism, avoiding waste of resources and environmental pollution

Pending Publication Date: 2022-07-01
山东合创环保科技有限公司 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a microbial agent formulation for soil improvement and a preparation method thereof, which solves the problem of insufficient stability of the antibacterial performance of the microbial agent in the process of soil improvement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] An embodiment of the present invention provides a microbial inoculum formulation for soil improvement, comprising the following raw materials by weight: 7 parts of domesticated Bacillus subtilis bacterial solution, 27 parts of soybean meal, 0.6 parts of neutral protease, 20 parts of cornmeal, and 3.5 parts of glucose , 6.5 parts of dipotassium hydrogen phosphate, 5 parts of potassium dihydrogen phosphate, 1 part of coarse salt, 0.4 part of magnesium sulfate, 0.2 part of manganese sulfate, 0.0175 part of defoamer, soybean meal provides protein and Amino acids, cornmeal and glucose provide nitrogen and carbon sources for Bacillus subtilis, dipotassium hydrogen phosphate and potassium dihydrogen phosphate provide phosphorus and potassium sources for Bacillus subtilis, and coarse salt, magnesium sulfate and manganese sulfate provide basic growth factors for Bacillus subtilis. Nutrition.

[0027] Neutral protease is endoprotease with 50000U / g enzyme activity, and defoamer is...

Embodiment 2

[0041] An embodiment of the present invention provides a microbial inoculum formulation for soil improvement, comprising the following raw materials by weight: 6 parts of domesticated Bacillus subtilis bacterial solution, 26 parts of soybean meal, 0.7 parts of neutral protease, 18 parts of cornmeal, and 4 parts of glucose , 7 parts of dipotassium hydrogen phosphate, 4.5 parts of potassium dihydrogen phosphate, 1.2 parts of coarse salt, 0.35 parts of magnesium sulfate, 0.25 parts of manganese sulfate, 0.017 parts of defoamer, soybean meal provides Bacillus subtilis with protein and Amino acids, cornmeal and glucose provide nitrogen and carbon sources for Bacillus subtilis, dipotassium hydrogen phosphate and potassium dihydrogen phosphate provide phosphorus and potassium sources for Bacillus subtilis, and coarse salt, magnesium sulfate and manganese sulfate provide basic growth factors for Bacillus subtilis. Nutrition.

[0042] Neutral protease is endoprotease with 50000U / g enzy...

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Abstract

The invention provides a microbial agent formula for soil improvement and a preparation method thereof, and relates to the technical field of microbial agents. The microbial agent formula for soil improvement is prepared from the following raw materials in parts by weight: 6 to 8 parts of domesticated bacillus subtilis bacterial liquid, 26 to 28 parts of soybean meal, 0.5 to 0.7 part of neutral protease, 18 to 22 parts of corn flour, 3 to 4 parts of glucose, 6 to 7 parts of dipotassium phosphate, 4.5 to 5.5 parts of monopotassium phosphate, 0.8 to 1.2 parts of crude salt, 0.35 to 0.45 part of magnesium sulfate, 0.15 to 0.25 part of manganese sulfate and 0.017 to 0.018 part of a de-foaming agent. Neutral protease with high enzyme activity is used for carrying out enzymolysis on soybean meal, bacillus subtilis is gradually subjected to variable-temperature shaking culture and high-salinity constant-temperature shaking culture, domesticated bacillus subtilis liquid with high temperature change resistance and salt tolerance is selected, and it is guaranteed that bacillus subtilis can stably and preferentially grow in soil; the effectiveness of the fungicide on soil improvement is ensured.

Description

technical field [0001] The invention relates to the technical field of microbial inoculants, in particular to a microbial inoculum formulation for soil improvement and a preparation method thereof. Background technique [0002] With the rapid development of science and technology, people's understanding of soil problems has gradually deepened. It has been found that the lack of rationality of fertilizer application plans and the abuse of bactericidal substances have led to a large number of beneficial microorganisms. The beneficial microorganisms in the soil cooperate with each other and form an antagonistic relationship with the harmful microorganisms to improve the soil. [0003] The strains of the existing microbial inoculants have insufficient tolerance to temperature changes, and the adaptability to saline-alkali soils is not high. The stability of the bacteriostatic effect is insufficient, and the soil aggregate structure cannot be effectively improved. SUMMARY OF T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36C09K17/40C12R1/125
CPCC12N1/20C12N1/36C09K17/40
Inventor 赵银岭袁新邢福国孔彦王德贤马世卓刘继州郭亮王晓倩
Owner 山东合创环保科技有限公司