Multifunctional nano-enzyme as well as preparation method and application thereof
A nano-enzyme and multi-functional technology, which is applied in the direction of nanotechnology, nanotechnology, chemical instruments and methods, etc., to achieve the effect of efficiently killing bacteria
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Embodiment 1
[0030] Example 1 Preparation of Cu-G Nanozyme
[0031] (1) guanine and copper nitrate were mixed and stirred in an aqueous solution with a mass ratio of 10:1 for 2 hours, and the water was removed by drying to obtain a mixed powder;
[0032] (2) The obtained mixed powder is ground and placed in a tube furnace, heated to 550°C at a heating rate of 2.2°C / min, and kept for another 4 hours;
[0033] (3) After returning to room temperature, grinding again, removing unreacted copper nitrate with pure water, and drying to obtain nano-enzyme material, the metal ions are encapsulated by guanine, and denoted as Cu-G nano-enzyme.
[0034] Transmission electron microscopy images and elemental distributions of Cu-G nanozymes are shown in figure 1 ,Depend on figure 1 It can be seen that the size of the sample is in the nanometer scale, and elements such as Cu, C, N, and O are uniformly dispersed on the surface of the material.
[0035] Test the peroxidase properties of Cu-G nanozymes:
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Embodiment 2
[0044] Example 2 Preparation of Fe-G Nanozyme
[0045] (1) guanine and ferrous sulfate were mixed and stirred in an aqueous solution with a mass ratio of 10:1 for 2 hours, and the water was removed by drying to obtain a mixed powder;
[0046] (2) The obtained mixed powder is ground and placed in a tube furnace, heated to 550°C at a heating rate of 2.2°C / min, and kept for another 4 hours;
[0047] (3) after recovering to room temperature, grind again, and remove unreacted copper nitrate with pure water, and dry to obtain nano-enzyme material, which is denoted as Fe-G nano-enzyme;
[0048](4) Similar to Cu-G, Fe-G has nanometer size and can generate hydroxyl radicals as peroxidase and glucose oxidase.
Embodiment 3
[0049] Example 3 Preparation of Cr-G Nanozyme
[0050] (1) guanine and chromium nitrate were mixed and stirred in an aqueous solution with a mass ratio of 10:1 for 2 hours, and the water was removed by drying to obtain a mixed powder;
[0051] (2) The obtained mixed powder is ground and placed in a tube furnace, heated to 550° C. at a heating rate of 2.2° C. / min, and kept for another 4 hours;
[0052] (3) After returning to room temperature, grind again, and remove unreacted copper nitrate with pure water, and dry to obtain nano-enzyme material, which is denoted as Cr-G nano-enzyme;
[0053] (4) Similar to Cu-G, Cr-G has nanometer size and can generate hydroxyl radicals as peroxidase and glucose oxidase.
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