Method for improving anaerobic fermentation performance of blue-green algae by using dielectric barrier discharge technology
A technology of dielectric barrier discharge and anaerobic fermentation, which is applied in the direction of microorganism-based methods, treatment of microorganisms with electricity/wave energy, fermentation, etc., to achieve the effect of increasing acid production
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Embodiment 1
[0080] The effect of dielectric barrier discharge process parameters on the dissolution of cyanobacteria.
[0081] Cyanobacterial cells are covered by a double-layered cell wall composed of peptidoglycan and cellulose, and there is often a gelatinous sheath composed of viscous polysaccharides on the outside. Its characteristic rigid structure results in less dissolved organic matter that can be converted during anaerobic digestion. , thus limiting the process of anaerobic fermentation. This study firstly investigated the effect of DBD's discharge voltage, discharge frequency and treatment time on cyanobacteria cracking. attached by figure 2 It can be seen that DBD treatment has a strong cracking effect on cyanobacteria, and the discharge voltage, discharge frequency and treatment time all have an impact on the dissolution effect of cyanobacteria. Overall, with the increase of discharge voltage, frequency and treatment time, the concentration of SCOD in the sludge supernatan...
Embodiment 2
[0084] Effects of Dielectric Barrier Discharge Pretreatment on Acid Production by Anaerobic Fermentation of Cyanobacteria
[0085] In this experiment, the effect of DBD treatment on the anaerobic fermentation performance of cyanobacteria was investigated. image 3 It can be seen that on the 8th day of anaerobic fermentation, the SCFAs production in the control group reached a maximum of 490 mg COD / L, and the DBD group reached a maximum of 1609 mg COD / L on the 8th day of anaerobic fermentation. On the sixth day of anaerobic fermentation, it was 3.3 times that of the control group. DBD pretreatment can promote the release of organic matter in cyanobacterial cells, increase the chance of anaerobic fermentation microorganisms contacting organic matter, and contribute to the rapid start of cyanobacterial anaerobic fermentation. Therefore, it can be considered that DBD pretreatment can not only improve the yield of SCFAs, but also shorten the time for anaerobic fermentation to reac...
Embodiment 3
[0087] Destructive effect of dielectric barrier discharge pretreatment on cyanobacterial cell structure;
[0088] To understand the degree of lysis of DBD-treated algal cells, the concentrations of DNA and LDH, which are thought to be closely related to cyanobacterial cell destruction, were determined as shown in the appendix. Figure 5 As shown, the detection concentration of DNA in the blank group was 3.3 mg / L, while in the DBD group when the treatment time was 10 min, 20 min and 30 min, the detection concentration of DNA increased to 17.7 mg / L, 28.1 mg / L and 36.2 mg / L . The detection concentration of LDH in the blank group was 2.2 mg / L, while the detection concentration of LDH increased to 22.1 mg / L, 26.3 mg / L and 42.8 mg / L when the treatment time of DBD group was 10 min, 20 min and 30 min. Compared with the control group, DBD treatment significantly increased the release of DNA and LDH (p2 O 2 ) plays a key role in disrupting algal structures. Discharge plasma oxidation...
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